2014
DOI: 10.1590/0074-0276140006
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Abstract: The Firmicutes bacteria participate extensively in virulence and pathological processes. Enterococcus faecalis is a commensal microorganism; however, it is also a pathogenic bacterium mainly associated with nosocomial infections in immunocompromised patients. Iron-sulfur [Fe-S] clusters are inorganic prosthetic groups involved in diverse biological processes, whose in vivo formation requires several specific protein machineries. Escherichia coli is one of the most frequently studied microorganisms regarding [F… Show more

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Cited by 4 publications
(2 citation statements)
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“…For each gene, the ratio of expression in I-DOMW, compared to that in N-DOMW, was normalized to the expression of the top two ranked constitutive genes in E. coli ( gapA and gyrB ). For E. faecalis , this ratio was normalized to the expression of rpoB and gyrB (Riboldi et al, 2014 ). To assess for reagent and genomic DNA contamination, no-template and no-reverse-transcriptase controls were included.…”
Section: Methodsmentioning
confidence: 99%
“…For each gene, the ratio of expression in I-DOMW, compared to that in N-DOMW, was normalized to the expression of the top two ranked constitutive genes in E. coli ( gapA and gyrB ). For E. faecalis , this ratio was normalized to the expression of rpoB and gyrB (Riboldi et al, 2014 ). To assess for reagent and genomic DNA contamination, no-template and no-reverse-transcriptase controls were included.…”
Section: Methodsmentioning
confidence: 99%
“…Accordingly, among up-DAPs, we found the first two subunits of quinol oxidase caa3-QoxB (B7HWC4) and QoxA (B7HWC3) (Figure 4)-alongside HemC (B7HQM4), which is involved in the heme subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate. The other up-DAPs included: (i) two sulfatases (B7HKE1 and B7HKV9) and one sulfate adenyltransferase Sat (B7HKE6) that regulate and contribute to the sulfate assimilation pathway [34], which is known to be upregulated in response to oxidative stress [35]. (ii) An enzyme involved in molybdopterin co-factor (MoCo) biosynthesis (MoeB, B7HW37) that contributes to maintaining intracellular sulfur and thiol homeostasis [36] and prevents ROS damage [37].…”
Section: Exoproteome Analysismentioning
confidence: 99%