Comparative analysis of the tissue inflammatory response in human cutaneous and disseminated leishmaniasis

Dantas, Marina Loyola; Oliveira, Juliana Menezes Gomes Cabral de; Carvalho, Lucas P.; Passos, Sara; Queiroz, Adriano; Guimarães, Luiz Henrique; Machado, Paulo Roberto Lima; Carvalho, Edgar M.; Arruda, Sérgio

doi:10.1590/0074-0276130312

Cited by 26 publications

(32 citation statements)

References 37 publications

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“…Initial studies showed that DL patients have more negative leishmania skin delayed type hypersensitivity test (LST) and an impairment in lymphocyte proliferation and in the production of interferon‐γ and TNF in supernatants of mononuclear cells stimulated with soluble leishmania antigen (SLA) as compared to CL patients . However, more recently, we document that cell‐mediated immune response at the lesion site of DL patients is similar to what is observed in CL ulcers . We believe that there is no impairment in T‐cell response in DL patients and raised the hypothesis that the poor T‐cell response observed in cells from peripheral blood was due to the migration of the majority of to the antigen reactive cells to the great number of lesions observed in DL patients .…”

Section: Introductionmentioning

confidence: 81%

“…8 However, more recently, we document that cell-mediated immune response at the lesion site of DL patients is similar to what is observed in CL ulcers. 6,9 We believe that there is no impairment in T-cell response in DL patients and raised the hypothesis that the poor T-cell response observed in cells from peripheral blood was due to the migration of the majority of to the antigen reactive cells to the great number of lesions observed in DL patients. 6 Moreover, these data suggest that parasite, more than host factors, may be involved in the pathogenesis of DL.…”

Section: Introductionmentioning

confidence: 98%

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Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function

Cardoso

Bezerra

Medina

et al. 2019

Parasite Immunology

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Summary Aims The polymorphism observed in Leishmania braziliensis is associated with different clinical forms of leishmaniasis. Neutrophils ( PMN s) participate in the pathogenesis of leishmania infection, and here, we evaluate neutrophil function after infection with isolates of L. braziliensis from cutaneous leishmaniasis ( CL ) or disseminated leishmaniasis ( DL ) patients. Methods and results Neutrophils from 30 healthy subjects ( HS ) were infected with isolates of L . ( V .) braziliensis obtained from three CL and three DL patients. They were infected at the ratio of 3:1 parasites per neutrophil, and leishmania uptake was evaluated by microscopy. The neutrophil activation markers and oxidative burst by expression of dihidrorhodamine ( DHR ) were evaluated by flow cytometry and cytokine production by ELISA . The frequency of infected cells and the number of amastigotes were higher in neutrophils infected with CL isolates compared to DL isolates ( P < 0.05). The DHR and CD 66b expression after infection with DL isolate was lower than with CL isolates. There was no difference regarding chemokine production. Conclusion The L. (V.) braziliensis isolates of DL induced lower respiratory burst and neutrophils activation markers compared with CL isolates which may contribute to parasite survival and dissemination in DL patients.

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Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 98%

Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function

Cardoso

Bezerra

Medina

et al. 2019

Parasite Immunology

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“…Firstly, sand fly- or needle-delivered promastigotes are converted into amastigotes within one to two days, when neutrophils are prevalent in the lesions. Secondly, neutrophils are ready detected in chronic forms of cutaneous leishmaniasis in patients infected with L. amazonensis, L. mexicana , or L. braziliensis [23,24] [25] [9]. At present, there is limited information on how neutrophils interact with these amastigotes.…”

Section: Discussionmentioning

confidence: 99%

Interactions between Neutrophils and <b><i>Leishmania braziliensis</i></b> Amastigotes Facilitate Cell Activation and Parasite Clearance

Carlsen¹,

Jie

Liang

et al. 2015

J Innate Immun

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Leishmania braziliensis and Leishmania amazonensis are both causative agents of cutaneous leishmaniasis in South America. However, patient prognosis and the host immune response differ considerably depending on the infecting parasite species. The mechanisms underlying these differences appear to be multifactorial, with both host and parasite components contributing to disease outcome. As neutrophils are a prominent component of the inflammatory infiltrate in chronic cutaneous, diffuse cutaneous and mucocutaneous lesions, we examined neutrophil activation and microbicidal activity against amastigotes of L. amazonensis and L. braziliensis. We found that murine neutrophils internalized L. braziliensis amastigotes with greater efficiency than did L. amazonensis amastigotes. Additionally, L. braziliensis infection was a potent trigger for neutrophil activation, oxidative burst, degranulation and the production of interleukin (IL)-22 and IL-10, while L. amazonensis amastigotes poorly induced these responses. Finally, neutrophils were able to kill L. braziliensis amastigotes, especially when cells were activated with phorbol myristate acetate. L. amazonensis amastigotes, however, were highly resistant to neutrophil microbicidal mechanisms. This study reveals, for the first time, differential neutrophil responsiveness to distinct species of Leishmania amastigotes and highlights the complexity of neutrophil-amastigote interactions during chronic leishmaniasis.

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“…Activation of macrophages by interferon (IFN)-γ + produced by CD4 + T cells contribute to control parasite growth,3,4 whereas CD8 + T cells have been associated with pathology 5–7. Histopathological studies in ulcers of L-CL patients show an increase in inflammatory response, with the participation of T cells, B cells, plasma cells, macrophages, and the development of a granuloma 8–11. Although an intense lymphocyte proliferation and production of IFN-γ and tumor necrosis factor is induced on Leishmania antigen stimulation of peripheral blood mononuclear cells from patients with L-CL,12 in the preulcerative phase of the disease, lymphocyte proliferation, and cytokine production is lower than in patients with L-CL 13.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis

Saldanha¹,

Queiroz²,

Machado³

et al. 2017

Am J Trop Med Hyg

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Cutaneous leishmaniasis (CL), characterized by an ulcerated lesion, is the most common clinical form of human leishmaniasis. Before the ulcer develops, patients infected with Leishmania (Viannia) braziliensis present a small papule at the site of the sandfly bite, referred to as early cutaneous leishmaniasis (E-CL). Two to four weeks later the typical ulcer develops, which is considered here as late CL (L-CL). Although there is a great deal known about T-cell responses in patients with L-CL, there is little information about the in situ inflammatory response in E-CL. Histological sections of skin biopsies from 15 E-CL and 28 L-CL patients were stained by hematoxilin and eosin to measure the area infiltrated by cells, as well as tissue necrosis. Leishmania braziliensis amastigotes, CD4+, CD8+, CD20+, and CD68+ cells were identified and quantified by immunohistochemistry. The number of amastigotes in E-CL was higher than in L-CL, and the inflammation area was larger in classical ulcers than in E-CL. There was no relationship between the number of parasites and magnitude of the inflammation area, or with the lesion size. However, there was a direct correlation between the number of macrophages and the lesion size in E-CL, and between the number of macrophages and necrotic area throughout the course of the disease. These positive correlations suggest that macrophages are directly involved in the pathology of L. braziliensis–induced lesions.

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Comparative analysis of the tissue inflammatory response in human cutaneous and disseminated leishmaniasis

Cited by 26 publications

References 37 publications

Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function

Leishmania braziliensis isolated from disseminated leishmaniasis patients downmodulate neutrophil function

Interactions between Neutrophils and <b><i>Leishmania braziliensis</i></b> Amastigotes Facilitate Cell Activation and Parasite Clearance

Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis

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