The rare enterovirus c99 and echovirus 29 strains in Brazil: potential risks associated to silent circulation

Luchs, Adriana; Leal, Élcio; Tardy, Kaelan; Milagres, Flavio Augusto de Pádua; Komninakis, Shirley Vasconcelos; Brustulin, Rafael; Teles, Maria da Aparecida Rodrigues; Lobato, Márcia Cristina Alves Brito Sayão; Chagas, Rogério Togisaki das; Abrão, Maria de Fátima Neves dos Santos; Soares, Cassia Vitória de Deus Alves; Deng, Xutao; Delwart, Eric; Sabino, Éster Cerdeira; Costa, Antônio Charlys da

doi:10.1590/0074-02760190160

Cited by 12 publications

(13 citation statements)

References 28 publications

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“…In Brazil, the follow‐up of the CNS infections associated to EV is not mandatory, except for acute flaccid paralysis syndrome (post‐polio surveillance system) 102 . Therefore, the real burden of non‐polio EV infection is underestimated in the country 103,104 . It is imperative to establish an effective non‐polio EV surveillance system in Brazil to allow the EV identification and associate them (or not) with preventable conditions, inappropriate treatment, and containment measures 105…”

Section: Discussionmentioning

confidence: 99%

Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil

Rocha

Estofolete

Milhim

et al. 2020

Journal of Medical Virology

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Enterovirus (EV) is commonly associated with central nervous system (CNS) syndromes. Recently, gastroenteric viruses, including rotavirus (RVA), human astrovirus (HAstV), and norovirus (NoV), have also been associated with CNS neurological disorders. The aim of the present study was to investigate the presence of EV, RVA, HAst, and NoV associated to CNS infections with undiagnosed etiology in Northwest region of São Paulo State, Brazil, and to conduct the molecular characterization of the positive samples detected. A total of 288 cerebrospinal fluid samples collected from July to December 2017 were tested for EV and NoV by quantitative real‐time polymerase chain reaction (RT‐qPCR), HAstV by conventional RT‐PCR, and RVA by enzyme‐linked immunosorbent assay. Positive‐EV samples were inoculated in cells lines, amplified by RT‐PCR and sequenced. RVA, NoV, and HAstV were not detected. EV infection was detected in 5.5% (16/288), and five samples successful genotyped: echovirus 3 (E3) (1/5), coxsackie virus A6 (CVA6) (1/5), and coxsackie virus B4 (CVB4) (3/5). Meningitis was the main syndrome observed (12/16; 75%). CVA6, CVB4, and E3 were identified associated with aseptic meningitis. Reports of CVA6 associated with aseptic meningitis are rare, E3 had not been previously reported in Brazil, and epidemiological data on CVB4 in the country is virtually unknown. The present investigation illustrates the circulation of diverse EV types in a small regional sample set and in a short period of time, highlighting the importance of an active EV surveillance system in CNS infections. Enhanced understanding of undiagnosed CNS infections will assist in public health and health care planning.

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Section: Discussionmentioning

confidence: 99%

Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil

Rocha

Estofolete

Milhim

et al. 2020

Journal of Medical Virology

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“…The samples described above were initially sent to the Public Health Laboratory of Tocantins (LACEN-TO) and stored at −20 °C, and the frozen faecal specimens were then taken to USP's Institute of Tropical Medicine (IMT/USP) to identify common enteric viruses, including RVAs, NoVs, HAstVs [32], and HSaVs [33], as well as rare [34][35][36] or potential new viruses [37][38][39][40][41][42], through NGS investigation.…”

Section: Study Populationmentioning

confidence: 99%

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

Tahmasebi

Luchs

Tardy

et al. 2020

Journal of General Virology

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Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8 % (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9 % (55/251) of samples and mixed infections in 37.4 % (94/251), with RVA/HAdV-F being the most frequent association (21.5 %; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.

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“…In Brazil, a limited number of studies on EV that are associated with AGE have been reported [11,12]. These works were based on description only one specific type, hence, the epidemiological analysis of EV infection in AGE patients is restricted.…”

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confidence: 99%

Detection and identification of enteroviruses circulating in children with acute gastroenteritis in Pará State, Northern Brazil (2010–2011)

Machado

Sousa

Monteiro

et al. 2020

Virol J

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Although acute gastroenteritis (AGE) has been reported as a common infectious disease in children, there is scarce information about enterovirus (EV) circulating associated with AGE cases in Brazil. The purpose of the present study was to identify and characterize the enteroviruses associated with AGE in children in Belém, Brazil. A total of 175 stool samples were obtained from children hospitalized revealing the presence of EV in 26.3% (46/175) of infections. EV type was identified in 78.3% (36/46) and EV-B species (61.1%; 22/36) was the most prevalent EV-detected followed by EV-C (25%; 9/36) and EV-A (13.9%; 5/36). This study has provided important information about the enterovirus circulation in Pará state, Northern Brazil.

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The rare enterovirus c99 and echovirus 29 strains in Brazil: potential risks associated to silent circulation

Cited by 12 publications

References 28 publications

Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil

Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

Detection and identification of enteroviruses circulating in children with acute gastroenteritis in Pará State, Northern Brazil (2010–2011)

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