2019
DOI: 10.1590/0074-02760180438
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Leishmania braziliensis is the etiological agent of American mucosal leishmaniasis, one of the most severe clinical forms of leishmaniasis. Here, we report the assembly of the L. braziliensis (M2904) genome into 35 continuous chromosomes. Also, the annotation of 8395 genes is provided. The public availability of this information will contribute to a better knowledge of this pathogen and help in the search for vaccines and novel drug targets aimed to control the disease caused by this Leishmania species.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
16
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
6
3
1

Relationship

3
7

Authors

Journals

citations
Cited by 16 publications
(16 citation statements)
references
References 28 publications
(27 reference statements)
0
16
0
Order By: Relevance
“…The fact that the duplication of LPG2 was not initially identified highlights limitations regarding the way Leishmania genomes are traditionally assembled, which usually entails the use of low-coverage whole-genome sequencing using short reads, with subsequent assembly performed using a reference genome obtained from a previously sequenced genome that can even belong to a different Leishmania species (Peacock et al, 2007 ). The presence of two copies of LPG2 in L. infantum limited our ability to perform genetic manipulation via homologous recombination in this parasite species, which emphasizes the need to improve the overall quality of Leishmania genomes, i.e., completeness and contiguity, preferentially through the use of recently employed hybrid sequencing strategies (short and long reads) (Gonzalez-de la Fuente et al, 2017 , 2019 ; Lypaczewski et al, 2018 ). In the end, we overcame this limitation through the use of the CRISPR/Cas9 system for genome editing.…”
Section: Discussionmentioning
confidence: 99%
“…The fact that the duplication of LPG2 was not initially identified highlights limitations regarding the way Leishmania genomes are traditionally assembled, which usually entails the use of low-coverage whole-genome sequencing using short reads, with subsequent assembly performed using a reference genome obtained from a previously sequenced genome that can even belong to a different Leishmania species (Peacock et al, 2007 ). The presence of two copies of LPG2 in L. infantum limited our ability to perform genetic manipulation via homologous recombination in this parasite species, which emphasizes the need to improve the overall quality of Leishmania genomes, i.e., completeness and contiguity, preferentially through the use of recently employed hybrid sequencing strategies (short and long reads) (Gonzalez-de la Fuente et al, 2017 , 2019 ; Lypaczewski et al, 2018 ). In the end, we overcame this limitation through the use of the CRISPR/Cas9 system for genome editing.…”
Section: Discussionmentioning
confidence: 99%
“…It contains transcriptomic and genomic data of several Leishmania species, including L . braziliensis [101]. On the other hand, LeishCyc can be used to study biochemical pathways (metabolomics) of Leishmania species using datasets of L .…”
Section: Useful Online Tools/databasesmentioning
confidence: 99%
“…Two fragments of 6535 and 4257 nucleotides belonging to the Leishmania braziliensis maxicircle were assembled [20], showing that despite the evolutionary divergence of L. braziliensis regarding other pathogenic Leishmania species [21], a remarkable conservation in the maxicircle gene ordering (synteny) was maintained. Now, when NGS technology is widely used for determining the nuclear genomic sequences of an increasing number of Leishmania species and strains [22,23,24,25,26,27,28,29,30,31,32], the time is coming to generate complete sequences of the mitochondrial genomes for the many Leishmania species named to date. In this work, we describe a pipeline designed to assemble the complete maxicircle sequences and the different minicircles by using the genomic NGS-reads generated during Leishmania nuclear genome sequencing.…”
Section: Introductionmentioning
confidence: 99%