2019
DOI: 10.1590/0074-02760180350
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Abstract: BACKGROUND The prompt diagnosis of plasmodial species for effective treatment prevents worsening of individual health and avoids transmission maintenance or even malaria reintroduction in areas where Plasmodium does not exist. Polymerase chain reaction (PCR) allows for the detection of parasites below the threshold of microscopic examination.OBJECTIVE Our aim was to develop a real-time PCR test to reduce diagnostic errors and increase efficacy.METHODS The lower limit of quantification and the linearity/analyti… Show more

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Cited by 4 publications
(2 citation statements)
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References 27 publications
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“…P. simium/P. vivax was diagnosed by conventional (18) and real-time (19) PCRs, and no P. falciparum or P. malariae infection was detected; (20,21) the same was true for microscopic examination.…”
mentioning
confidence: 89%
“…P. simium/P. vivax was diagnosed by conventional (18) and real-time (19) PCRs, and no P. falciparum or P. malariae infection was detected; (20,21) the same was true for microscopic examination.…”
mentioning
confidence: 89%
“…Yine Almeida-de-Oliveira ve ark. (27) tarafından yapılan bir çalışmada, SYBR TM ve TaqMan TM prob sistemleri kullanılarak uygulanan GZ-PZR yönteminde P. vivax için limit saptama performansı 0.01 parazit/µl olarak bulunmuştur.…”
Section: Bulgularunclassified