Dengue serotype-specific immune response in Aedes aegypti and Aedes albopictus

Smartt, Chelsea T.; Shin, Dong Ho; Alto, Barry W.

doi:10.1590/0074-02760170182

Cited by 26 publications

(28 citation statements)

References 29 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disconnect between kinetics in intermediate tissues and those of the salivary glands suggests that there are either stochastic processes driving kinetics in early tissues that do not predict how many viruses make it to the salivary glands, or that factors affecting replication rate in the salivary glands are somewhat independent [23]. For example, previous research has demonstrated that the strength and efficacy of immunity varies across tissues [23] and in response to different serotypes [41]. It is also possible that DENV is modulating the vector immune response in a strain- or tissue-dependent manner [42].…”

Section: Discussionmentioning

confidence: 99%

Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti

et al. 2019

View full text Add to dashboard Cite

Dengue virus (DENV) transmission by mosquitoes is a time-dependent process that begins with the consumption of an infectious blood-meal. DENV infection then proceeds stepwise through the mosquito from the midgut to the carcass, and ultimately to the salivary glands, where it is secreted into saliva and then transmitted anew on a subsequent bite. We examined viral kinetics in tissues of the Aedes aegypti mosquito over a finely graded time course, and as per previous studies, found that initial viral dose and serotype strain diversity control infectivity. We also found that a threshold level of virus is required to establish body-wide infections and that replication kinetics in the early and intermediate tissues do not predict those of the salivary glands. Our findings have implications for mosquito GMO design, modeling the contribution of transmission to vector competence and the role of mosquito kinetics in the overall DENV epidemiological landscape.

show abstract

Section: Discussionmentioning

confidence: 99%

Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The related gene expression level was normalized to the expression of the Ae. aegypti ribosomal protein S7 gene (GenBank Accession # AY380336) [ 45 ]. The levels of gene expression were compared to non-infectious blood-fed mosquitoes.…”

Section: Methodsmentioning

confidence: 99%

Effect of Oral Infection of Mayaro Virus on Fitness Correlates and Expression of Immune Related Genes in Aedes aegypti

Alto

Civana

Wiggins

et al. 2020

Viruses

Self Cite

View full text Add to dashboard Cite

Mayaro virus is a mosquito-borne Alphavirus endemic to forests of tropical South America with a sylvatic cycle involving non-human primates and Haemagogus mosquitoes. Human infection with Mayaro virus causes a febrile illness and long-lasting arthralgia and cases are often associated with exposure to tropical forest habitats. Human movement between tropical forest habitats and urban settings may allow for imported cases and subsequent local transmission by domestic mosquito Aedes aegypti. The relative importance of Ae. aegypti as a vector of Mayaro virus may depend on the pathogenic effects of the virus on fitness correlates, especially those entomological parameters that relate to vectorial capacity. We performed mosquito infection studies and compared adult survival and fecundity of females from Brazilian and Floridian populations of Ae. aegypti following oral ingestion of uninfectious (control) and Mayaro virus infectious blood. Mayaro virus infected and refractory mosquitoes had similar or 30–50% lower fecundity than control (unexposed) mosquitoes, suggesting a reproductive cost to mounting an immune response or phenotypic expression of refractoriness. Survival of adult female mosquitoes and targeted gene expression in the Toll and IMD pathways were not altered by Mayaro virus infection. Adult lifespan and fecundity estimates were independent of measured viral titer in the bodies of mosquitoes. The lack of adverse effects of infection status on female survival suggests that Mayaro virus will not alter vectorial capacity mediated by changes in this parameter.

show abstract

“…The factor which leads to the drastic increase in the prevalence of the disease is rapid urbanization with poor water and drainage management. 4 Urban poverty in India tops 25 percent; about 81 million people stay below the poverty line in metropolitan areas. In India, the urban poor are counted out by rural ones.…”

Section: Introductionmentioning

confidence: 99%

Effect of community-based intervention on awareness of dengue and its prevention among urban poor community in India: a systematic review approach

Lachyan

Khan

Zaki

et al. 2020

Int J Community Med Public Health

View full text Add to dashboard Cite

Owing to increased epidemic activity and difficulties in controlling the insect vector, dengue has become a major public health problem globally. The prevention and control of dengue rely mainly on vector control methods. A systematic review was conducted using four databases (PubMed, Cochrane, Google Scholar and Scopus) and a manual search of the reference lists of the identified studies. Data from included studies were extracted, analyzed. Applying all inclusion and exclusion criteria 23 articles was included. Further relevant articles using this keywords Aedes dengue breeding habits housing and community intervention were selected. Eight studies combined community participation programme with dengue control tools. Findings of the published literature indicate that at baseline, almost half the respondents did not know that dengue is serious but preventable, or that it is transmitted by mosquitoes. The analysis showed that dengue vector control is carried out by vertically structured programmes of national, state, and local administrative bodies through fogging and larval control, without any involvement of community-based organizations, and that vector control efforts were conducted in an isolated and irregular way. The most productive container types for Aedes pupae were cement tanks, drums, and discarded containers. Evidence that community-based dengue control programmes alone and in combination with other control activities can enhance the effectiveness of dengue control programmes is weak. This review demonstrates a paucity of reliable evidence for the effectiveness of any dengue vector control method. Standardized studies of higher quality to evaluate and compare methods must be prioritized to optimize cost-effective dengue prevention. Clear best practice guidelines for the methodology of entomological studies should be developed.

show abstract

Dengue serotype-specific immune response in Aedes aegypti and Aedes albopictus

Cited by 26 publications

References 29 publications

Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti

Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti

Effect of Oral Infection of Mayaro Virus on Fitness Correlates and Expression of Immune Related Genes in Aedes aegypti

Effect of community-based intervention on awareness of dengue and its prevention among urban poor community in India: a systematic review approach

Contact Info

Product

Resources

About