2018
DOI: 10.1590/0074-02760170071
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Abstract: In occult hepatitis B infection (OBI), hepatitis B virus DNA (HBV DNA) can be detected in serum samples; however, oral fluid collection for detection of HBV DNA has not yet been explored, despite the availability of collection devices. Serum and oral fluid samples from 45 hepatitis B core antibody (anti-HBc)-positive patients were collected for the amplification of the HBV polymerase gene. HBV DNA was detected in five serum and four oral fluid samples (the detection limit for oral fluid was 1.656 log IU/mL in … Show more

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Cited by 6 publications
(7 citation statements)
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References 15 publications
(15 reference statements)
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“…Oral fluid samples collected with Salivette were also evaluated for detection of HBV DNA among patients with occult hepatitis B infection (OBI) [26]. In this study, it was possible to detect HBV in 4 saliva from 5 OBI cases and it was not detected in the individual that had low viral load (1.623 log IU / mL) in serum.…”
Section: Oral Fluid Samples For Infectious Disease Diagnosismentioning
confidence: 99%
“…Oral fluid samples collected with Salivette were also evaluated for detection of HBV DNA among patients with occult hepatitis B infection (OBI) [26]. In this study, it was possible to detect HBV in 4 saliva from 5 OBI cases and it was not detected in the individual that had low viral load (1.623 log IU / mL) in serum.…”
Section: Oral Fluid Samples For Infectious Disease Diagnosismentioning
confidence: 99%
“…The qPCR in DBS samples presented good agreement (86.40%), sensitivity (77.63%) and excellent specificity (100%) compared to serum samples. In-house qPCR was used as reference since this method was previously optimized and compared to commercial methods 25 Some positive samples by commercial method were excluded to compare the results obtained in DBS what could impact in the estimation of sensitivity and specificity of the assay in this study and it is considered a limitation of the present work. Some studies carried out in DBS showed higher sensitivity values 26 29 , however most of them were carried out using commercial methods and with a smaller sample size.…”
Section: Discussionmentioning
confidence: 99%
“… 9 However, the sensitivity of the ELISA is too low for screening HBV infection at early stage. 25 In recent years, qPCR has become the standard assay for HBV screening in clinical setting for its wide dynamic range of viral detection (range from 10 to 10 8 IU per reaction), specificity, and pollution control. 6 , 26 , 27 Comparing of enzyme immunoassay assay, qPCR could reduce time for identification of HBV after exposure from an average of approximately 32 days (HBsAg marker) to 15 days (HBV DNA marker).…”
Section: Discussionmentioning
confidence: 99%
“…In the current study, the reaction time of the HBV-MCDA was also optimized. Four reaction times (25,35,45, 55 min) were tested and compared. The HBV-MCDA reaction was carried out as described above, and the amplification products were monitored using LFB.…”
Section: Optimization Of the Amplification Time For Hbv-mcda-lfb Assaymentioning
confidence: 99%
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