Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

Requena-Castro, Rocío; Reyes‐López, Miguel Ángel; Rodríguez-Reyna, Rosa Eminé; Palma-Nicolás, José Prisco; Bocanegra-García, Virgilio

doi:10.1590/0074-02760160468

Cited by 6 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DENV panel confirmed the serotypes of 12 RT-PCR-serotyped patient samples or clinical isolates (Fig 3F, Fig. S25), and identified 2 clinical isolates with mixed infection, a commonly observed phenomenon(18). SHERLOCK can therefore be extended to differentiate between related viruses or serotypes using a single amplification reaction.…”

mentioning

confidence: 72%

Field-deployable viral diagnostics using CRISPR-Cas13

Myhrvold

Freije

Gootenberg

et al. 2018

Science

1,008

1,017

View full text Add to dashboard Cite

Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field-deployable. Here, we demonstrate that the Cas13-based SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations down to 1 copy/μl. We develop HUDSON (Heating Unextracted Diagnostic Samples to Obliterate Nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENV detection directly from patient samples in < 2 hours. We further demonstrate that SHERLOCK can distinguish the 4 DENV serotypes as well as region-specific strains of ZIKV from the 2015–2016 pandemic. Finally, we report the rapid design and testing (<1 week) of instrument-free assays to detect clinically relevant viral single nucleotide polymorphisms.

show abstract

mentioning

confidence: 72%

Field-deployable viral diagnostics using CRISPR-Cas13

Myhrvold

Freije

Gootenberg

et al. 2018

Science

1,008

1,017

View full text Add to dashboard Cite

show abstract

“…Two earlier studies from India, including ours, and one from Mexico used this PCR for identifying viremia among serologically diagnosed patients with dengue. DENV RNA positivity was found to be 48.6% (53/109)and 52% (71/136) among NS1 ELISA positives or 59.5% among 117 NS1 and 4 IgM positives (immunochromatographic rapid test) . Overall, detection of DENV RNA among NS1‐positive patients with dengue was approximately 50% when CprM region–based PCR was used for screening.…”

Section: Discussionmentioning

confidence: 99%

Risk of transfusion‐associated dengue: screening of blood donors from Pune, western India

et al. 2018

View full text Add to dashboard Cite

BACKGROUND: Dengue, a mosquito-borne viral disease, is endemic in >125 countries worldwide. The threat of blood-borne transmission of dengue virus (DENV) has been documented. STUDY DESIGN AND METHODS:This study was conducted to assess the potential magnitude of transfusion-associated dengue, by determination of DENV seromarkers in blood donations from Pune, India, during two dengue seasons (2016 and 2017). These included DENV nonstructural protein 1 (NS1), anti-DENV immunoglobulin (Ig) M, anti-DENV IgG (enzyme-linked immunosorbent assay), and DENV RNA (reverse transcription-polymerase chain reaction).RESULTS: NS1 (IgM) reactivity was 1 of 209, 0.48% (11/209, 5.3%) in 2016 and 2 of 311, 0.64% (20/311, 6.4%) in 2017. Of the 34 NS1/IgM reactives, 1 NS1-reactive donor and 10 IgM-reactive donors exhibited evidence of secondary infection. DENV RNA was not detected in any of the 34 NS1/IgM reactives. Among the NS1/IgM negatives, anti-DENV IgG reactivity was high in 2016 (75%) and further increased in 2017 (87%, p = 0.002). CONCLUSION:Although RNA negative, detection of 34 NS1/IgM-reactive donations, of which 11 had evidence of secondary infection, suggests the need for further evaluation on the basis of potential risk to recipients of either dengue transmission or increased risk of secondary infection. These would include multicenter studies followed by cost-benefit analyses. ABBREVIATIONS: DENV = dengue virus; ELISA = enzyme-linked immunosorbent assay; Ig = immunoglobulin; PMC = Pune Municipal Corporation; RT-PCR = reverse transcription-polymerase chain reaction; TAD = transfusion-associated dengue From the

show abstract

“…We computed sensitivity and specificity of RT-PCR [33,35,[68][69][70][71][72][73][74][75][76], NS1 ELISA [75][76][77][78][79][80], NS1 rapid diagnostic tests [81][82][83][84][85][86], IgM ELISA [87,89,90] and IgM RDT [88,91] 100%, 95% CI against 80.3-99%, 95%CI. These observations concur with the results reported earlier [94].…”

Section: Evaluation Of Sensitivity and Specificitymentioning

confidence: 99%

“…The final version of the manuscript was revised by all authors prior to submission. Tables Table 1: Articles that reported different dengue laboratory diagnoses (n=51)* Diagnostic test Dengue virus isolation [29], [30] RT-PCR [33], [34], [35], [36], [37], [38], [39], [68] [50], [52], [53], [77], [78], [79], [80], [81] a NS1 RDT [83], [84], [85], [86], [87] and [88] IgM ELISA [54], [89], [91],and [ *Source of data: PubMed, Web of Science and Google Scholar databases.…”

Section: Ethical Approval and Consented To Participatementioning

confidence: 99%

Challenges and Opportunities of Laboratory diagnosis of Dengue virus infection: a review

Mwanyika

Misinzo

Rugarabamu

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Globally, dengue is one of the most important mosquito-borne viral diseases. Lack of effective vaccines and specific therapy against the disease threaten global health. Reliance on clinical diagnosis is complex due to clinical manifestations which resemble other diseases. This review examined various challenges of current dengue laboratory diagnoses, emerging technological opportunities and highlights considerations for future dengue diagnoses. Methods: A literature search from PubMed, Web of Science and Google Scholar databases was done from October 2018 to January 2019. Thematic descriptive analysis was done for all qualitative data and quantitative data analysis for computation of sensitivity and specificity of selected diagnostic tests at 95% confidence was done using R software (v3.4.4, mada package). The results: A total of 128 articles was reviewed. The current dengue laboratory diagnoses include (i) virus isolation (ii) detection of nucleic acid (iii) detection of non-structural protein 1(NS1) antigen and (iv) detection of anti-dengue antibodies. Assessment of diagnostic performance shows that reverse transcription-polymerase chain reaction (RT-PCR) and IgM antibody capture enzyme-linked immunosorbent assay (IgM ELISA) have high and consistent sensitivity (82.6% to 99.2% and 92.8% to 97.8%, respectively) and specificity (78.8% to 100% and 80.3% to 99%, respectively) compared to NS1 ELISA and NS1 commercial rapid tests with sensitivity (53.7% to 96.2% and 49.7% to 99.5%, respectively) and specificity (34.5% to 93.8% and 63.8% to 98.6%, respectively). Major challenges of dengue laboratory diagnosis include lack of reliable tests for routine purposes. Routine dengue tests are mainly serological, which are not suitable for discriminating dengue virus from other infecting flaviviruses due to cross-reactivity, narrow window of diagnosis due to short virus life cycle and inconsistent performance of commercial rapid tests. New technologies such as biosensors and nanobodies are being developed to improve sensitivity, specificity, detection time and reduce the cost. However, the performance of these new tools under field condition is unknown. Conclusion: Currently, RT-PCR and IgM ELISA are the most sensitive and specific dengue diagnostic tests, despite their limitations. Future research should explore emerging technologies to improve the sensitivity and specificity of dengue diagnostics.

show abstract

Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

Cited by 6 publications

References 9 publications

Field-deployable viral diagnostics using CRISPR-Cas13

Field-deployable viral diagnostics using CRISPR-Cas13

Risk of transfusion‐associated dengue: screening of blood donors from Pune, western India

Challenges and Opportunities of Laboratory diagnosis of Dengue virus infection: a review

Contact Info

Product

Resources

About