Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Yamashiro, Sandra; Fiúza, Vagner Ricardo da Silva; Teixeira, Ângela Therezinha Lauand Sampaio; Branco, Nilson; Levy, Carlos Emílio; Castro, Isabel Cristina Vidal Siqueira de; Franco, Regina Maura Bueno

doi:10.1590/0074-02760160435

Cited by 21 publications

(6 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…bieneusi DNA has already been identified in fecal samples in Brazil collected from humans (FENG et al, 2011), cattle (FIUZA et al, 2016b), birds (CUNHA et al, 2016LALLO et al, 2012), pigs (FIUZA et al, 2015 and sheep (FIUZA et al, 2016a). In addition, microsporidium was also detected in a sample of treated effluent water collected in the region of Campinas, São Paulo, demonstrating the significant possibility of dispersion of this agent even after sewage treatment (YAMASHIRO et al, 2017). However, epidemiologic factors and the occurrence of clinical disease in the country are still uncertain and need further clarification.…”

Section: Discussionmentioning

confidence: 92%

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Prado

Ramos

Fiúza

et al. 2019

Rev. Bras. Parasitol. Vet.

Self Cite

View full text Add to dashboard Cite

Enterocytozoon bieneusi is an opportunistic intestinal pathogen that infects humans and a wide variety of animals worldwide. Our aim in this study was to investigate the occurrence of E. bieneusi in a domestic cat population in Campo Grande, Mato Grosso do Sul, Brazil. Sixty fecal samples from diarrheic cats were subjected to polymerase chain reaction (PCR) and the amplicons were sequenced for identification. E. bieneusi was detected in two samples (3.3%), both identified as genotype D. This genotype has already been reported in animals and humans and is considered a zoonotic genotype. Our findings represent the first report of E. bieneusi in domestic cats in Brazil, reinforcing the importance of identifying this agent as a source of infection in animals and humans.

show abstract

Section: Discussionmentioning

confidence: 92%

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Prado

Ramos

Fiúza

et al. 2019

Rev. Bras. Parasitol. Vet.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The prevalence of E. bieneusi in rodents ranged from 1.1% to 100% (Table 4) 12,19,[28][29][30] . As proposed in other studies, factors contributing to the prevalence of these pathogens may include the examination method, age, sex, season, host health status, feeding density, sample size, geo-ecological conditions, and living conditions [31][32][33] .…”

Section: Discussionmentioning

confidence: 98%

Occurrence and genetic characteristics of Cryptosporidium spp. and Enterocytozoon bieneusi in pet red squirrels (Sciurus vulgaris) in China

Deng

Chai

Luo

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Cryptosporidium spp. and Enterocytozoon bieneusi are two well-known protist pathogens which can result in diarrhea in humans and animals. To examine the occurrence and genetic characteristics of Cryptosporidium spp. and E. bieneusi in pet red squirrels (Sciurus vulgaris), 314 fecal specimens were collected from red squirrels from four pet shops and owners in Sichuan province, China. Cryptosporidium spp. and E. bieneusi were examined by nested PCR targeting the partial small subunit rRNA (SSU rRNA) gene and the ribosomal internal transcribed spacer (ITS) gene respectively. The infection rates were 8.6% (27/314) for Cryptosporidium spp. and 19.4% (61/314) for E. bieneusi. Five Cryptosporidium species/genotypes were identified by DNA sequence analysis: Cryptosporidium rat genotype II (n = 8), Cryptosporidium ferret genotype (n = 8), Cryptosporidium chipmunk genotype III (n = 5), Cryptosporidium rat genotype I (n = 4), and Cryptosporidium parvum (n = 2). Additionally, a total of five E. bieneusi genotypes were revealed, including three known genotypes (D, SCC-2, and SCC-3) and two novel genotypes (RS01 and RS02). Phylogenetic analysis revealed that genotype D fell into group 1, whereas the remaining genotypes clustered into group 10. To our knowledge, this is the first study to report Cryptosporidium spp. and E. bieneusi in pet red squirrels in China. Moreover, C. parvum and genotype D of E. bieneusi, previously identified in humans, were also found in red squirrels, suggesting that red squirrels may give rise to cryptosporidiosis and microsporidiosis in humans through zoonotic transmissions. These results provide preliminary reference data for monitoring Cryptosporidium spp. and E. bieneusi infections in pet red squirrels and humans.Cryptosporidium spp. and Enterocytozoon bieneusi, causative agents of cryptosporidiosis and microsporidiosis, are two important opportunistic intestinal pathogens that can infect vertebrate and invertebrate, posing a significant threat to public health 1 . Humans are infected with these pathogens mainly via the fecal-oral route with anthroponotic and zoonotic transmission or via food-borne and water-borne transmission 2,3 . Clinical manifestations of infection with these pathogens are often inconsistent due to the variabilities in the health condition of infected hosts 4,5 . In healthy individuals, these pathogens usually cause asymptomatic infection or self-limiting diarrhea 6 . However, infection may also result in chronic or life-threatening diarrhea in immunocompromised individuals, such as patients with acquired immunodeficiency syndrome and patients who had undergone organ transplantation 7 . In addition to humans, there are a variety of animals that can act as hosts for these two pathogens, including various mammals, reptiles, birds, amphibians and insects 8 . Therefore, Cryptosporidium spp. and

show abstract

“…; Yamashiro et al. ), in fresh produce sold at retail level in Poland (Jedrzejewski et al. ), and in milk from dairy herds in Korea (Lee ).…”

Section: Discussionmentioning

confidence: 99%

“…Enterocytozoon bieneusi has a worldwide distribution and the ability to infect humans as well as a broad range of domestic and wild animals (Sant ın 2015). It has been identified in multiple water sources (Cotte et al 1999;Galv an-D ıaz et al 2014;Guo et al 2014;Yamashiro et al 2017), in fresh produce sold at retail level in Poland (Jedrzejewski et al 2007), and in milk from dairy herds in Korea (Lee 2008). Enterocytozoon bieneusi was responsible of a foodborne outbreak of gastrointestinal illness associated with the consumption of contaminated cucumbers in Sweden (Decraene et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain

Santı́n

Calero‐Bernal

Carmena

et al. 2017

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Microsporidia comprises a diverse group of obligate intracellular parasites that infect a broad range of invertebrates and vertebrates. Among Microsporidia, Enterocytozoon bieneusi is the most frequently detected species in humans and animals worldwide bringing into question the possible role of animal reservoirs in the epidemiology of this pathogen. Although E. bieneusi is an emerging zoonotic pathogen able to infect many domestic and wild mammals that could act as reservoir of infection for humans and other animals, only few studies have documented its occurrence in wild carnivores. To determine the occurrence of E. bieneusi in wild carnivores, we examined 190 wild carnivores collected from different locations in Spain. Twenty-five fecal samples (13.2%) from three host species (European badger, beech marten, and red fox) were E. bieneusi-positive by PCR. Nucleotide sequence analysis of the ITS region revealed a high degree of genetic diversity with a total of eight distinct genotypes including four known (PtEbIX, S5, S9, and WildBoar3) and four novel (EbCar1-EbCar4) genotypes identified. Phylogenetic analysis showed that the four novel genotypes (EbCar1-EbCar4), S5, S9, and WildBoar3 clustered within the previously designated zoonotic Group 1. Our results demonstrate that human-pathogenic genotypes are present in wild carnivores, corroborating their potential role as a source of human infection and environmental contamination.

show abstract

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Cited by 21 publications

References 54 publications

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

Occurrence and genetic characteristics of Cryptosporidium spp. and Enterocytozoon bieneusi in pet red squirrels (Sciurus vulgaris) in China

Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain

Contact Info

Product

Resources

About