Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase

Arantes, Thales Domingos; Theodoro, Raquel Cordeiro; Teixeira, Marcus de Melo; Bagagli, Eduardo

doi:10.1590/0074-02760160374

Cited by 10 publications

(8 citation statements)

References 32 publications

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“…lutzii parasitic forms in the biological material of infected patients cannot distinguish between the two species. Therefore, the identification of the involved species requires culture isolation and application of molecular techniques 56,57 . …”

Section: Laboratory Examinations For Specific Diagnosismentioning

confidence: 99%

Brazilian guidelines for the clinical management of paracoccidioidomycosis

Shikanai-Yasuda

Mendes

Colombo

et al. 2017

Rev. Soc. Bras. Med. Trop.

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289

514

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Paracoccidioidomycosis is a systemic fungal disease occurring in Latin America that is associated with rural environments and agricultural activities. However, the incidence and prevalence of paracoccidiodomycosis is underestimated because of the lack of compulsory notification. If paracoccidiodomycosis is not diagnosed and treated early and adequately, the endemic fungal infection could result in serious sequelae. While the Paracoccidioides brasiliensis (P. brasiliensis) complex has been known to be the causal agent of paracoccidiodomycosis, a new species, Paracoccidioides lutzii (P. lutzii), has been reported in Rondônia, where the disease has reached epidemic levels, and in the Central West and Pará. Accurate diagnoses and availability of antigens that are reactive with the patients' sera remain significant challenges. Therefore, the present guidelines aims to update the first Brazilian consensus on paracoccidioidomycosis by providing evidence-based recommendations for bedside patient management. This consensus summarizes etiological, ecoepidemiological, molecular epidemiological, and immunopathological data, with emphasis on clinical, microbiological, and serological diagnosis and management of clinical forms and sequelae, as well as in patients with comorbidities and immunosuppression. The consensus also includes discussion of outpatient treatments, severe disease forms, disease prevalence among special populations and resource-poor settings, a brief review of prevention and control measures, current challenges and recommendations.

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Section: Laboratory Examinations For Specific Diagnosismentioning

confidence: 99%

Brazilian guidelines for the clinical management of paracoccidioidomycosis

Shikanai-Yasuda

Mendes

Colombo

et al. 2017

Rev. Soc. Bras. Med. Trop.

Self Cite

289

514

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“…directly in the environmental samples [ 139 ]. Furthermore, Arantes et al [ 140 ] tried to extend the use of FISH for clinical purposes. The main goal was to launch a versatile technique to detect and differentiate Paracoccidioides spp.…”

Section: Molecular Diagnosis: Where Do We Stand?mentioning

confidence: 99%

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

Pinheiro

Hahn

Camargo

et al. 2020

JoF

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Paracoccidioidomycosis (PCM) is a mycotic disease caused by the Paracoccidioides species, a group of thermally dimorphic fungi that grow in mycelial form at 25 °C and as budding yeasts when cultured at 37 °C or when parasitizing the host tissues. PCM occurs in a large area of Latin America, and the most critical regions of endemicity are in Brazil, Colombia, and Venezuela. The clinical diagnosis of PCM needs to be confirmed through laboratory tests. Although classical laboratory techniques provide valuable information due to the presence of pathognomonic forms of Paracoccidioides spp., nucleic acid-based diagnostics gradually are replacing or complementing culture-based, biochemical, and immunological assays in routine microbiology laboratory practice. Recently, taxonomic changes driven by whole-genomic sequencing of Paracoccidioides have highlighted the need to recognize species boundaries, which could better ascertain Paracoccidioides taxonomy. In this scenario, classical laboratory techniques do not have significant discriminatory power over cryptic agents. On the other hand, several PCR-based methods can detect polymorphisms in Paracoccidioides DNA and thus support species identification. This review is focused on the recent achievements in molecular diagnostics of paracoccidioidomycosis, including the main advantages and pitfalls related to each technique. We discuss these breakthroughs in light of taxonomic changes in the Paracoccidioides genus.

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“…On the other hand, it is the definitive way to attempt identification of the involved species in association with molecular techniques. 125,126…”

Section: Mycological Diagnosismentioning

confidence: 99%

New Insights on Pulmonary Paracoccidioidomycosis

Queiroz-Telles

Peçanha-Pietrobom

Júnior

et al. 2020

Semin Respir Crit Care Med

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Paracoccidioidomycosis (PCM) is one of the most relevant systemic endemic mycoses in Latin American countries, especially in South American countries, with endemic and hyperendemic areas. The real burden of PCM may be underestimated because of a lack of compulsory case notification. Recent phylogenetic data revealed that Paracoccidioides brasiliensis comprises several cryptic species including P. brasiliensis and P. lutzii. However, the genetic biodiversity of Paracoccidioides does not affect the clinical manifestations or therapeutic response to therapy. Lung involvement is a common finding, especially in patients experiencing the chronic form of the disease, and, because of its similarities with tuberculosis, clinicians must be alert to the possibility of PCM in patients with chronic respiratory manifestations and epidemiological risk factors for this fungal disease.

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Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase

Cited by 10 publications

References 32 publications

Brazilian guidelines for the clinical management of paracoccidioidomycosis

Brazilian guidelines for the clinical management of paracoccidioidomycosis

Molecular Tools for Detection and Identification of Paracoccidioides Species: Current Status and Future Perspectives

New Insights on Pulmonary Paracoccidioidomycosis

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