Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis

Primon-Barros, Muriel; Rigo, Graziela Vargas; Frasson, Amanda Piccoli; Santos, Odelta dos; Smiderle, Lisiane; Almeida, Silvana; Macêdo, Alexandre José; Tasca, Tiana

doi:10.1590/0074-02760150076

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…The ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability was also evaluated. We found a reduction in activity and an augment in ADA gene expression after iron restriction by 2,2-bipyridyl and ferrozine chelators [119] . These data support the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signaling and it is implicated in establishing infection and parasite survival.…”

Section: Involvement Of the Purinergic Signaling In Trichomoniasis Immentioning

confidence: 68%

Trichomoniasis immunity and the involvement of the purinergic signaling

Menezes

Tasca

2016

Biomedical Journal

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Innate and adaptive immunity play a significant role in trichomoniasis, the most common non-viral sexually transmitted disease worldwide. In the urogenital tract, innate immunity is accomplished by a defense physical barrier constituted by epithelial cells, mucus, and acidic pH. During infection, immune cells, antimicrobial peptides, cytokines, chemokines, and adaptive immunity evolve in the reproductive tract, and a proinflammatory response is generated to eliminate the invading extracellular pathogen Trichomonas vaginalis. However, the parasite has developed complex evolutionary mechanisms to evade the host immune response through cysteine proteases, phenotypic variation, and molecular mimicry. The purinergic system constitutes a signaling cellular net where nucleotides and nucleosides, enzymes, purinoceptors and transporters are involved in almost all cells and tissues signaling pathways, especially in central and autonomic nervous systems, endocrine, respiratory, cardiac, reproductive, and immune systems, during physiological as well as pathological processes. The involvement of the purinergic system in T. vaginalis biology and infection has been demonstrated and this review highlights the participation of this signaling pathway in the parasite immune evasion strategies.

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Section: Involvement Of the Purinergic Signaling In Trichomoniasis Immentioning

confidence: 68%

Trichomoniasis immunity and the involvement of the purinergic signaling

Menezes

Tasca

2016

Biomedical Journal

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“…Under serum restriction conditions, adenosine deaminase enzymatic activity does not change, while E-5'N had an increased gene expression; it acts as a compensatory pathway, increasing adenosine levels. Under low iron availability con-ditions, adenosine deaminase decreases its activity, which returns to normal conditions when there is exposure to iron sources [96,97]. Nucleotides also influence virulence factors.…”

Section: Immunomodulatory Weapons: T Vaginalis and Purinergic Signali...mentioning

confidence: 99%

Infinity war: Trichomonas vaginalis and interactions with host immune response

Galego¹,

Tasca²

2023

Microb Cell

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Trichomonas vaginalis is the pathological agent of human trichomoniasis. The incidence is 156 million cases worldwide. Due to the increasing resistance of isolates to approved drugs and clinical complications that include increased risk in the acquisition and transmission of HIV, cervical and prostate cancer, and adverse outcomes during pregnancy, increasing our understanding of the pathogen’s interaction with the host immune response is essential. Production of cytokines and cells of innate immunity: Neutrophils and macrophages are the main cells involved in the fight against the parasite, while IL-8, IL-6 and TNF-α are the most produced cytokines in response to this infection. Clinical complications: T. vaginalis increases the acquisition of HIV, stimulates the invasiveness and growth of prostate cells, and generates an inflammatory environment that may lead to preterm birth. Endosymbiosis: Mycoplasma hominis increased cytotoxicity, growth, and survival rate of the parasite. Purinergic signaling: NTPD-ases and ecto-5’-nucleotidase helps in parasite survival by modulating the nucleotides levels in the microenvironment. Antibodies: IgG was detected in serum samples of rodents infected with isolates from symptomatic patients as well as patients with symptoms. However, antibody production does not protect against a reinfection. Vaccine candidate targets: The transient receptor potential- like channel of T. vaginalis (TvTRPV), cysteine peptidase, and α-actinin are currently cited as candidate targets for vaccine development. In this context, the understanding of mechanisms involved in the host-T. vaginalis interaction that elicit the immune response may contribute to the development of new targets to combat trichomoniasis.

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“…A ausência de mitocôndrias é acompanhada de uma necessidade de ferro maior que a de outros protistas (Weinberg 1974;Schneider et al 2011;Beltrán et al 2013). Assim como outros microrganismos, o T. vaginalis depende do ferro para uma série de ações em seu metabolismo (Gorrell 1985;De Jesus et al 2006;Kühn 2009;Vieira et al 2015), inclusive ao regular a expressão gênica de algumas proteínas de superfície (Lehker et al 1991;Lehker & Alderete 1992;Primon-Barros et al 2015), que atuam como mecanismo de resistência à lise pelo complemento (Alderete et al 1995;Arroyo et al 2015). A importância do ferro para o parasito pode explicar, em parte, a maior prevalência da infecção entre as mulheres (Secor et al 2014), uma vez que elas têm em seu trato genital uma disponibilidade mais elevada desse íon, o que é relacionado ao fluxo menstrual.…”

Section: Morfologia E Metabolismo Do Trichomonas Vaginalisunclassified

Prevalência de Trichomonas vaginalis em mulheres na idade fértil atendidas em equipe de saúde da família do Distrito Federal

Glehn¹

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Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis

Cited by 6 publications

References 42 publications

Trichomoniasis immunity and the involvement of the purinergic signaling

Trichomoniasis immunity and the involvement of the purinergic signaling

Infinity war: Trichomonas vaginalis and interactions with host immune response

Prevalência de Trichomonas vaginalis em mulheres na idade fértil atendidas em equipe de saúde da família do Distrito Federal

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