Molecular characterization of Mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping

Feyisa, Seifu Gizaw; Haeili, Mehri; Zahednamazi, Fatemeh; Mosavari, Nader; Taheri, Mohammad Mohammad; Hamzehloo, Gholamreza; Zamani, Samin; Feizabadi, Mohammad Mehdi

doi:10.1590/0037-8682-0405-2015

Cited by 20 publications

(7 citation statements)

References 19 publications

(14 reference statements)

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“…In addition, indirect Spoligotyping was performed by our group on 172 M.tb isolates, and in none of the strains, genotype MANU2 was observed (Unpublished data). Although two studies have suggested the presence of MANU2 in M.tb cultures in Iranian patients, but the percentage of this genotype in these two articles is very small in compare to our study (4.2 and 7.9% versus 35.7%) [ 31 , 32 ].…”

Section: Main Textcontrasting

confidence: 93%

Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

et al. 2018

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ObjectiveBased on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere with the results or not.ResultsBased on the Spoligotyping pattern, among the fourteen patients, 57.1% had different genotypes in clinical samples and their respective cultures. These discrepant patterns were suggestive of polyclonal infections in clinical samples with possible overlapping Spoligotype patterns. We propose that in societies with high mixed infections (e.g. Iran), direct Spoligotyping on clinical samples can be controversial.

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Section: Main Textcontrasting

confidence: 93%

Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

et al. 2018

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“…It is possible that Central Asian Strain (CAS) family of TB, especially the SIT26 strain, might have been transported from Pakistan to the Sistan-Baluchestan province of Iran as this province shares geographic borders with Afghanistan and Pakistan where the CAS family is predominant. It has also been reported that due to the large numbers of immigrants living in Iran from Afghanistan, a country enlisted as one of the 22 high burden TB countries in the world, it is believed that there are considerable numbers of cases of active, latent, and multiple drug-resistant TB infection among Afghani refugees, and legal or illegal immigrants in Iran 6,7,33 . Therefore, case detection efforts should be focused on migrant populations whose origin are from MDR-TB strain dominant countries, such as Afghanistan and Pakistan, in order to curb the continued MDR-TB transmission.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of and risk factors for multidrug-resistant tuberculosis in Iran and its neighboring countries: systematic review and meta-analysis

Jimma

Ghazisaeedi

Shahmoradi

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Tuberculosis, in particular drug-resistant tuberculosis, is of global concern due to the high mortality and morbidity associated with it annually. The aim of this study was to determine the prevalence of and the risk factors for multidrug-resistant tuberculosis in Iran and its neighboring countries. Four databases (PubMed, BioMed Central, EMBASE, and Web of Science) were searched using key terms. Nineteen eligible articles were identified, of which 12 and seven were used for quantitative and qualitative analysis, respectively. The overall pooled estimate of the prevalence of multidrug-resistant tuberculosis, including both new and previously treated tuberculosis cases, in Iran, Iraq, Turkey and Pakistan was 16% (95% confidence interval [CI] 11-20). The patients with a previous tuberculosis treatment history (odds ratio [OR] = 6.45; 95% CI 5.12-7.79), those aged <45 years (OR = 1.57; 95% CI 1.12-2.03), and those who were males (OR = 1.83; 95% CI 1.19-2.48) had an increased pool risk of developing multidrug-resistant tuberculosis. The forest plot revealed that the pooled odds for the development of multidrug- resistant tuberculosis were 2.01 (95% CI 1.65-2.36). Poor adherence to treatment was one of the predictors of unsuccessful treatment outcomes. Multidrug-resistant tuberculosis is a great concern for public health programs in many countries globally, including those included in this review. The risk factors for the development of multidrug-resistant tuberculosis, specifically a previous tuberculosis treatment history, should be targeted through the implementation of specialized interventions.

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“…MTB molecular markers promoted the development of reproducible genotyping methods [4], including insertion sequence 6110 (IS6110), restriction fragment length polymorphism (RFLP) typing [5], spacer oligonucleotide typing (spoligotyping) [6], single nucleotide polymorphism analysis [7],mycobacterial interspersed repetitive unit variable number tandem repeats (MIRU-VNTRs) assessment [8], large sequence polymorphism (LSP) typing [9], and genome [10] sequence analysis. IS6110-RFLP has been the gold standard for genotyping MTB since 1993, but this procedure is time consuming, technically demanding and labour intensive.…”

Section: Introductionmentioning

confidence: 99%

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

et al. 2018

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BackgroundTuberculosis remains a serious threat to human health as an infectious disease in China. Henan, a most populated province in China, has a high incidence of tuberculosis (TB). Though the genetic diversity of Mycobacterium tuberculosis (MTB) has been investigated in many regions, there have been only a few studies on the molecular characteristics and drug resistance phenotypes in Henan. This is the first study on the genetic profile of MTB from Henan.MethodsA total of 668 MTB isolates from various areas were genotyped with spoligotyping and 26-locus MIRU-VNTR (classical 24-locus MIRU-VNTR and 2 other loci). The association between TB spoligotype signatures and drug-resistant profiles was analysed.ResultsOur data revealed that MTB isolates circulating in Henan had a high degree of genetic variation. The Beijing family was the most predominant genotype (83.53%,n = 558), and the typical Beijing type(ST1) was the major sublineage (81.73%,n = 546). In total,668 isolates were divided into 567 different types, forming 38 clusters (2–15 isolates per cluster), and 529 unique types by 26-locus MIRU-VNTR analysis. There was no correlation between the Beijing family and gender, age at diagnosis or treatment history, whereas the Beijing family was significantly associated with all four first-line drug resistance and multidrug-resistant phenotypes. For these samples, 15 of 26 MIRU-VNTR loci had high or moderate discriminatory power according to the Hunter-Gaston discriminatory index. A combination of the 10 most polymorphic loci had similar discriminatory power as the 26-locus set.ConclusionThe Beijing genotype is the most prevalent family. Ten-locus MIRU-VNTR in combination with spoligotyping can efficiently classify the molecular type of MTB in Henan Province.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3351-y) contains supplementary material, which is available to authorized users.

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Molecular characterization of Mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping

Cited by 20 publications

References 19 publications

Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

Prevalence of and risk factors for multidrug-resistant tuberculosis in Iran and its neighboring countries: systematic review and meta-analysis

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

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