Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children

Soroush, Setareh; Jabalameli, Fereshteh; Taherikalani, Morovat; Eslampour, Mohammad Amin; Beigverdi, Reza; Emaneini, Mohammad

doi:10.1590/0037-8682-0384-2016

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…hominis were biofilm producer and only 2 isolates (11.11%) were non-biofilm producers, and these results similar to those obtained by (Garza-González et al, 2011;Soroush et al, 2017). Of the 16 biofilm producer isolates, 14 isolates (77.77%) were categorized as strong biofilm producers and 2 (11.11%) isolates were identified as moderate biofilm producers (Figure 4) as defined by (Christensen et al, 1985;Mathur et al, 2006) , and these results accordance with those…”

Section: Biofilm Formation In S Hominis Isolatessupporting

confidence: 90%

Detection of Biofilm Producer Methicillin Resistant Staphylococcus hominis Isolated From Different Clinical Sources

Mustafa¹

2018

ZJPAS

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Staphylococcus hominis is an opportunistic gram positive bacteria and is a member of coagulase-negative staphylococci (CoNS) . During the study, 213 clinical specimens were collected from patients admitted to different hospitals in Erbil city, Iraq. Only 18(8.45%) isolates of S. hominis were isolated including 7 isolates (17.07%) from blood, 4 isolates (8.70%) from urine, 3 isolates (7.14%) from ear, 2 isolates (6.67%) from wound, and one isolate from each nasal swab (4.55%) and oral cavity (3.13%), all S. hominis identified based on morphology, cultural, biochemical tests, and further confirmed by Vitek 2 compact system. To determine the most accurate assay for measuring methicillin resistance S. hominis, compared the detection of mecA by PCR with detection by National Committee for Clinical Laboratory Standards methods using oxacillin and Cefoxitin disk diffusion method. The results of oxacillin showed 13 (72.22%) isolates resistant to methicillin and 5 (27.77%) isolates were sensitive to it. While, the results of cefoxitin demonstrated that 16 (88.89%) isolates were resistant to methicillin and only 2 isolates (11.11%) were sensitive to it. However, the same results of the Cefoxitin disk diffusion method was obtained by PCR and by using mecA gene which 16 isolates (88.89%) were carried mecA gene with product size 499bp. The results of microtiter plate method revealed that 16 (88.89%) isolates of S. hominis were biofilm producer and only 2 isolates (11.11%) were non-biofilm producer. Moreover,of 16 biofilm producer isolates, 14 isolates (77.77%) were categorized as strong biofilm producers and 2 (11.11%) isolates were identified as moderate biofilm producers.

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Section: Biofilm Formation In S Hominis Isolatessupporting

confidence: 90%

Detection of Biofilm Producer Methicillin Resistant Staphylococcus hominis Isolated From Different Clinical Sources

Mustafa¹

2018

ZJPAS

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“… 14 In addition, other studies report the formation of biofilm as a characteristic virulent factor for coagulase‐negative bacteria such as S. hominis and S. epidermidis . 11 , 12 The primary mechanism of resistance reported in coagulase‐negative staphylococcus is the possession of the mec A gene. 29 In this study, 72.5% of the 51 cefoxitin‐resistant isolates were positive for the mec A gene.…”

Section: Discussionmentioning

confidence: 99%

“…Recent reports revealed that S. hominis strains harbor many resistant genes and may have several mechanisms for its pathogenicity, including adhesion to epithelial cells and the invasion activity of extracellular toxins, causing damage to the host epithelium 14 . In addition, other studies report the formation of biofilm as a characteristic virulent factor for coagulase‐negative bacteria such as S. hominis and S. epidermidis 11,12 . The primary mechanism of resistance reported in coagulase‐negative staphylococcus is the possession of the mec A gene 29 .…”

Section: Discussionmentioning

confidence: 99%

“…However, some virulent factors such as biofilm formation, that complicate infected wound management have been identified with the coagulase‐negative bacteria. 11 , 12 …”

Section: Introductionmentioning

confidence: 99%

“…However, some virulent factors such as biofilm formation, that complicate infected wound management have been identified with the coagulase-negative bacteria. 11,12 Staphylococcus hominis is one of the major Staphylococcus species found on the human skin and is mainly found in the axillae, perineal and inguinal areas. 13 S. hominis is ranked third among the coagulasenegative bacteria that are of clinical importance.…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study

Kini

Wireko

Osei-Poku

et al. 2023

Health Science Reports

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Background and Aim Filarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. Staphylococcus hominis , a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that S. hominis could assume a different behavior in filarial lymphedema patients. To this end, we investigated the levels of antibiotic resistance and extent of mec A gene carriage in S. hominis among individuals presenting with filarial lymphedema in rural Ghana. Method We recruited 160 individuals with stages I–VII lymphedema, in a cross‐sectional study in the Ahanta West District of the Western Region of Ghana. Swabs from lymphedematous limb ulcers, pus, and cutaneous surfaces were cultured using standard culture‐based techniques. The culture isolates were subjected to Matrix‐Assisted Laser Desorption/Ionization Time of Flight (MALDI‐TOF) mass spectrometry for bacterial identification. Antimicrobial susceptibility testing (AST) was performed using the Kirby–Bauer method. mec A genes were targeted by polymerase chain reaction for strains that were cefoxitin resistant. Results In all, 112 S . hominis were isolated. The AST results showed resistance to chloramphenicol (87.5%), tetracycline (83.3%), penicillin (79.2%), and trimethoprim/sulphamethoxazole (45.8%). Of the 112 strains of S. hominis , 51 (45.5%) were resistant to cefoxitin, and 37 (72.5%) of the cefoxitin‐resistant S. hominis haboured the mec A gene. Conclusion This study indicates a heightened level of methicillin‐resistant S. hominis isolated among filarial lymphedema patients. As a result, opportunistic infections of S. hominis among the already burdened filarial lymphedema patients in rural Ghana may have reduced treatment success with antibiotics.

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Sessein and isosessein with anti-inflammatory, antibacterial and antioxidant activity isolated from Salvia sessei Benth

Gómez-Rivera

González-Cortazar²,

Herrera-Ruíz³

et al. 2018

Journal of Ethnopharmacology

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Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mec analysis of methicillin resistant Staphylococcus hominis from blood cultures of children

Abstract: The results indicate that MRSHo with high antibiotic resistance and unknown SCCmec might become a serious problem in the future for the treatment of patients such as children.

Cited by 7 publications

References 25 publications

Detection of Biofilm Producer Methicillin Resistant Staphylococcus hominis Isolated From Different Clinical Sources

Detection of Biofilm Producer Methicillin Resistant Staphylococcus hominis Isolated From Different Clinical Sources

Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study

Sessein and isosessein with anti-inflammatory, antibacterial and antioxidant activity isolated from Salvia sessei Benth

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