Coexistence of virulence genes in methicillin-resistant Staphylococcus aureus clinical isolates

Rossato, Adriana Medianeira; Reiter, Keli Cristine; d’Azevedo, Pedro Alves

doi:10.1590/0037-8682-0339-2017

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…Our result was lower than the proportion of PVL-positive S. aureus reported in Maiduguri, North East Nigeria (52.1%) [13]; the 61.4% in the Gambia [25], the 58% in Sudan [26], the 73.91% in Colombia [27], the 61.5% in India [28], the 56.2% in Iran [29], the 46.3% in Kazakhstan [30] and the 62.5% in Trinidad and Tobago [31]. In contrast, our result was higher than the prevalence of PVL-positive strains reported from several other studies in Abuja, Nigeria that reported a prevalence of 10.7% among isolates obtained from Maitama District Hospital [14], the 12.7% prevalence reported in Kuwait [8], the 23% reported in Iran [32] and the 18.6% prevalence reported in Brazil [33]. These studies suggest geographical differences in the prevalence of PVL-positive S. aureus.…”

Section: Discussioncontrasting

confidence: 89%

Prevalence and genotypic characteristics of Panton-Valentine Leukocidin-producing Staphylococcus aureus isolates obtained in Jos, North Central Nigeria

Essien¹,

Boswihi²,

Agbakoba³

et al. 2021

World J. Adv. Res. Rev.

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Background: Staphylococcus aureus is an opportunistic pathogen that colonizes and causes infections in humans. Panton Valentine Leukocidin (PVL) is a cytolytic toxin produced by some strains of S. aureus and are mostly associated with skin and soft tissue infections and necrotizing pneumonia. Aim: To investigate the prevalence and genotypic characteristics of PVL-positive S. aureus strains cultured from patients in three tertiary hospitals in Jos, Nigeria. Methods: Two hundred and fourteen clinical S. aureus isolates were obtained from three tertiary hospitals in Jos. Polymerase chain reaction was used to detect lukSF-PV gene that encodes PVL, and sensitivity to antimicrobial agents was performed on PVL-positive S. aureus. Genotypic characteristics of the PVL-positive S. aureus was determined by spa typing and multilocus sequence typing (MLST). Results: The genes for PVL were detected in 67/214 (31.3%) of S. aureus isolates. Majority of the PVL-positive isolates were obtained from wound (n=37; 55.2%), blood (n=11; 16.4%) and urine (n=10; 14.9). Most of PVL-positive isolates (n=58; 34.7%) were methicillin sensitive S. aureus (MSSA) while nine isolates (19.1%) were methicillin resistant S. aureus (MRSA). Spa typing identified 14 different spa types, dominated by t355 (n=33; 49.3%), followed by t174 (n=7; 10.4%), t019 and t159 (n=5; 7.5%). MLST revealed six sequence types (ST) namely, ST152 (n=35), ST121 (n=9), ST1 (n=8), ST30 (n=8), ST772 (n=6) and ST15 (N=1). Conclusion: This study revealed that 31.3% of S. aureus isolated in Jos hospitals carried genes for PVL, belonged to six sequence types and 14 spa types with t355-ST152-MSSA as the dominant genotype.

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Section: Discussioncontrasting

confidence: 89%

Prevalence and genotypic characteristics of Panton-Valentine Leukocidin-producing Staphylococcus aureus isolates obtained in Jos, North Central Nigeria

Essien¹,

Boswihi²,

Agbakoba³

et al. 2021

World J. Adv. Res. Rev.

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“…All isolates were subject to PCR in order to detect one (or more) of the 18 genes coding for staphylococcal enterotoxins ( sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser and seu) [ 63 ]. We also screened the isolates to ascertain the presence of genes encoding for any exfoliative toxins ( siet, eta, etb ), hemolysins ( hla, hlb, hld, hlg and hlgv ), the tst gene encoding for the TSST, the luk S– luk F genes encoding for PVL leukocidin, the luk E– luk D genes encoding the bicomponent leukotoxin LukE–LukD and the luk M gene encoding leukocidin M. Adhesin factors, including clumping factors ( clf A, clf B) and fibronectin-binding protein ( fnb A, fnb B); binding proteins, including fibrinogen-binding protein ( fib ), collagen-binding protein ( cbp ), bone sialoprotein-binding protein ( bsp ), laminin-binding protein ( lamin ), elastin-binding protein ( ebp ); and genes encoding capsules ( cap 5, cap 8) were also tested ( Supplementary Table S1 ) [ 64 , 65 ].…”

Section: Methodsmentioning

confidence: 99%

First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia

Chehida

Gharsa

Tombari

et al. 2021

Animals

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A total of 318 nasal and rectal swabs were collected from 159 apparently healthy camels (Camelus dromedarius) randomly selected from five regions in southern and central Tunisia and screened for Staphylococcus aureus carriage. Staphylococcus spp. were recovered from 152 of 159 camels studied (95.6%) and in total 258 swabs (81%) were positive. Among these isolates, 16 were coagulase positive Staphylococcus (CoPS) (6.2%) and were characterized by biochemical and molecular tests as S. aureus. These were isolated from 14 camels (8.8%) with co-carriage in nasal and rectal mucosa by two camels. All S. aureus isolates recovered were methicillin-susceptible Staphylococcus aureus (MSSA) and were characterized by spa typing and PFGE. Three different spa types were recovered: t729, t4013 and a spa type newly registered as t19687, which was the most common. PFGE analysis revealed seven different patterns and these were characterized by MLST, which revealed five different sequence types (ST6, ST88, ST3583 and two new sequences, ST6504 and ST6506). All isolates harbored different virulence genes, including hld, encoding delta hemolysin; lukE–lukD, encoding bicomponent leukotoxin LukE–LukD; the clfB gene, encoding clumping factor B; the laminin gene, encoding laminin-binding protein; and cap8, encoding capsule type 8. Fifteen isolates harbored hemolysin beta (hlb) and fourteen encoded hemolysin alpha (hla) and hemolysin G2 (hlgv). Adhesin factors, including clfA and fnbB, were detected in five and four isolates respectively. Binding proteins, including collagen (cbp) and elastin-binding protein (ebp), were detected in two S. aureus isolates while fibrinogen-binding protein (fib) was identified in four isolates. This study provides the first set of genotyping data on the population structure and presence of toxin genes of S. aureus strains in Tunisian camels.

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“…3). Abdalrahmanet al (2015) found the incidence of hla gene was (75.6%) in the 168 Staph.aureus isolates from poultry, also, Rossato et al (2018) detected hla gene in 87.6% from 177 nosocomial MRSA strains isolated from patients.…”

Section: Discussionmentioning

confidence: 94%

Molecular detection of antibiotic resistance and virulence genes in staphylococcus species isolated from human and poultry

Ahmed

Al-azeem

2020

SVU-International Journal of Veterinary Sciences

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Staphylococcus species are the most precarious micro-organisms in poultry meat, because they cause multiple health problems, risks to consumers and the economy of the country. Since they act as antibiotic resistant bacteria that carry virulence genes and antibiotic resistance genes which can be transmitted from food producing animals as poultry to humans through various modes the major one being food chain and cause health hazards for the consumers. Therefore, the current study was aimed to investigate the incidence rate of Staphylococcus species isolated from broiler chicken and hospitalized patient, detection the antimicrobial susceptibility of these isolates by using Vitek2 system and confirmation the presence of genes encoding for pathogenicity and antimicrobial resistance in detected isolates by PCR. To achieve these 200 samples were collected from poultry farms and hospitalized patient (100 for each) in dissimilar districts in Assiut province, to be subjected to bacteriological examination. The results showed that the incidence of Staphylococcus species was 35% and 45% in poultry and human samples respectively on mannitol agar. Vitek2 system divided poultry isolates to 11 isolates as coagulasepositive Staphylococci (CoPS) and 24 isolates as coagulase-negative staphylococci (CoNS). Whereas 20 human isolates alienated as CoPS and 25 isolates assigned as CoNS. Antibiogram cleared that 45.7% and 53.3% of Staphylococcus isolates from poultry and human were identified as methicillin-resistant Staphylococci respectively, also Staphylococcal spp. cleared a resistance to different types of antimicrobials such as penicillin, tetracycline clindamycin. Vitek2 system showed a significant ability to differentiate among Staphylococcus species and distinguish its antimicrobial susceptibility which was complicated by conventional method. PCR results showed that the Staphylococcal isolates of poultry and human were harboring genes encoding for pathogenicity (coa, hld and pvl) and antimicrobial resistance (mecA, vanA, cfr and BlaZ) while none of the isolates harbored sei and seh genes. So the obtained results emphasized the importance of reducing the unwarranted use of antimicrobial agents and application of sanitary hygienic measures in poultry production.

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Coexistence of virulence genes in methicillin-resistant Staphylococcus aureus clinical isolates

Abstract: Horizontal transfer of virulence genes is very common in S. aureus, as suggested by the frequent coexistence of several virulence genes.

Cited by 8 publications

References 13 publications

Prevalence and genotypic characteristics of Panton-Valentine Leukocidin-producing Staphylococcus aureus isolates obtained in Jos, North Central Nigeria

Prevalence and genotypic characteristics of Panton-Valentine Leukocidin-producing Staphylococcus aureus isolates obtained in Jos, North Central Nigeria

First Report of Antimicrobial Susceptibility and Virulence Gene Characterization Associated with Staphylococcus aureus Carriage in Healthy Camels from Tunisia

Molecular detection of antibiotic resistance and virulence genes in staphylococcus species isolated from human and poultry

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