Monoterpene isolated from the essential oil of Trachyspermum ammi is cytotoxic to multidrug-resistant Pseudomonas aeruginosa and Staphylococcus aureus strains

Hosseinkhani, Farideh; Jabalameli, Fereshteh; Banar, Maryam; Abdellahi, Nafiseh; Taherikalani, Morovat; Leeuwen, Willem B. van; Emaneini, Mohammad

doi:10.1590/0037-8682-0329-2015

Cited by 16 publications

(14 citation statements)

References 18 publications

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“…As mentioned before, the results regarding Chinese cinnamon should be carefully interpreted. To our knowledge, this is the first description of ajowan essential oil activity against the tested MRSA strains, LMG 15975 and 16217, although previous literature already mentioned an antibacterial activity against E. coli LMG 8223 [ 27 , 28 ], P. aeruginosa LMG 6395 [ 28 , 29 , 30 ] and MSSA LMG 8064 [ 31 ], as observed in the present study. For the Chinese cinnamon, who is cytotoxic in both tested cell lines, our results presented a better MIC than the one described by Kaskatepe et al (2016) [ 32 ] against the same strain of P. aeruginosa LMG 6395 and the one described against E. coli LMG 8223 [ 33 , 34 ].…”

Section: Discussionsupporting

confidence: 71%

Antibacterial and Cytotoxic Activities of Ten Commercially Available Essential Oils

et al. 2020

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There is a huge concern in the medical field concerning the emergence of bacterial resistance to antibiotics. Essential oils are a source of antibacterial compounds that can overcome this problem. Ten essential oils that are commercially available were investigated in the present study: ajowan, basil, German chamomile, Chinese cinnamon, coriander, clove, lemongrass, Spanish lavender, oregano and palmarosa. Their direct, synergistic and indirect antibacterial activities were evaluated against different human pathogenic Gram-positive and Gram-negative strains. To evaluate their possible use in clinics, the cytotoxicity of these essential oils was also tested on keratinocyte and epithelial cell lines. Except for the Chinese cinnamon, coriander and lemongrass, all other essential oils presented no cytotoxicity at 32 and 16 μg/mL. The highest indirect antibacterial activities were observed with the palmarosa and Spanish lavender in association with penicillin V. These two associations presented a 64-fold decrease against a resistant strain of Staphylococcus aureus, however, at a cytotoxic concentration. It can also be highlighted that when tested at a non-cytotoxic concentration, the activity of oregano in association with penicillin V presented an eight-fold decrease. These results show the interest to use essential oils in combination with antibiotics to reduce their concentrations inside drugs.

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Section: Discussionsupporting

confidence: 71%

Antibacterial and Cytotoxic Activities of Ten Commercially Available Essential Oils

et al. 2020

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“…This finding is of utmost importance, since it demonstrates a promising potential of those two bioactive substances as antimicrobial agents tested against medically resistant strain. Hosseinkhani et al (2016) [ 37 ] also obtained promising results when testing the antibacterial activity of an essential oil rich in monoterpenes against multidrug resistant strains of S. aureus . According to the authors, the results may indicate the possibility of using monoterpenes incorporated into antimicrobial formulations in order to treat infections caused by multi-resistant bacteria.…”

Section: Discussionmentioning

confidence: 99%

Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus

Vasconcelos

Melo

Cavalcante

et al. 2017

BMC Complement Altern Med

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BackgroundThe emergence of multidrug-resistant bacteria is a worldwide concern and in order to find an alternative to this problem, the occurrence of antimicrobial compounds in Plectranthus amboinicus essential oil was investigated. Thus, this study aims to determine susceptibility of Staphylococcus aureus isolated from food to antibiotics, P. amboinicus essential oil (PAEO) and carvacrol.MethodsLeaves and stem of P. amboinicus were used for extraction of essential oil (PAEO) by hydrodistillation technique and EO chemical analysis was performed by gas chromatography coupled to a mass spectrometer. S. aureus strains (n = 35) isolated from food and S. aureus ATCC 6538 were used to evaluate the antimicrobial and antibiofilm activity of PAEO and carvacrol. All strains (n = 35) were submitted to antimicrobial susceptibility profile by disk diffusion method. Determination of MIC and MBC was performed by microdilution technique and antibiofilm activity was determined by microtiter-plate technique with crystal violet assay and counting viable cells in Colony Forming Units (CFU).ResultsCarvacrol (88.17%) was the major component in the PAEO. Antibiotic resistance was detected in 28 S. aureus strains (80%) and 12 strains (34.3%) were oxacillin and vancomycin-resistant (OVRSA). From the 28 resistant strains, 7 (25%) showed resistance plasmid of 12,000 bp. All strains (n = 35) were sensitive to PAEO and carvacrol, with inhibition zones ranging from 16 to 38 mm and 23 to 42 mm, respectively. The lowest MIC (0.25 mg mL−1) and MBC (0.5 mg mL−1) values were observed when carvacrol was used against OVRSA. When a 0.5 mg mL−1 concentration of PAEO and carvacrol was used, no viable cells were found on S. aureus biofilm.ConclusionThe antibacterial effect of carvacrol and PAEO proves to be a possible alternative against planktonic forms and staphylococcal biofilm.

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“…Five authors tested the essential oil of T. ammi against the reference strain ATCC 29 213 [14,28,48,51,53]. This provides a good example of how results are sometimes not comparable.…”

Section: Disparity In the Results And Importance Of All The Mentionedmentioning

confidence: 99%

“…However, all the strains, including the reference ATCC strain, showed the same MIC (< 0.02 v/v), which is very odd given the differences observed in the resistances. The authors did not comment on this unusual result [28]. Even in the case of a reference strain, it could be interesting to check if its resistance is within the ranges observed for a reported sensitive or resistant S. aureus.…”

Section: Ext/cmmentioning

confidence: 89%

“…Another important control that should always be done is a positive control, with either an antibiotic or a compound known to be active against the selected strain. For example, Hosseinkhani et al [28] used thymol as a positive control, but unfortunately, the results of its MIC against the tested strains are not mentioned. This is also the case of other authors [47,50].…”

Section: Controls: Unheeded and Yet So Importantmentioning

confidence: 99%

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Proposals for Antimicrobial Testing Guidelines Applied on Ajowan and Spanish Lavender Essential Oils

Ribeiro

Fraselle

Baudoux³

et al. 2021

Planta Med

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To fight the rising resistance of microorganisms to antibiotics, a strategy followed by several researchers is to focus on natural compounds, such as essential oils, as a source of potent antibacterial compounds. These last decades, hundreds of original papers have been written about microbiological assays that prove the antibacterial activity of essential oils and their use in the medical field. But can we really compare all the data available in the literature when the raw material, the microbiological assays, and/or the strains are different from one article to another? This review will point out the differences and the inadequate practices found in published articles that tested 2 lesser-studied essential oils–Spanish lavender and the ajowan–by the broth dilution method against Staphylococcus aureus, a human pathogenic bacterium. Many pitfalls were found in the literature, for example, a variable chemical composition rarely underlined by the authors, unidentified strains or clinical strains used without a related antibiogram, a lack of quality controls, and the assertion of questionable positive results. At last, some general guidelines that should be followed by every scientific researcher will be discussed.

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Monoterpene isolated from the essential oil of Trachyspermum ammi is cytotoxic to multidrug-resistant Pseudomonas aeruginosa and Staphylococcus aureus strains

Cited by 16 publications

References 18 publications

Antibacterial and Cytotoxic Activities of Ten Commercially Available Essential Oils

Antibacterial and Cytotoxic Activities of Ten Commercially Available Essential Oils

Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus

Proposals for Antimicrobial Testing Guidelines Applied on Ajowan and Spanish Lavender Essential Oils

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