Seropositivity for Rickettsia spp. and Ehrlichia spp. in the human population of Mato Grosso, Central-Western Brazil

Bezerra, Maria Cristina Fuzari; Melo, Andréia Lima Tomé; Taques, Ísis Indaiara Gonçalves Granjeiro; Aguiar, Daniel Moura de; Pacheco, Richard C.; Slhessarenko, Renata Dezengrini

doi:10.1590/0037-8682-0318-2016

Cited by 8 publications

(3 citation statements)

References 16 publications

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“…Further, they require specific treatment (doxycycline) that is not used for empiric treatment of acute febrile illness.As advanced diagnostic methods are employed to identify etiologic agents of undifferentiated fever, rickettsial infections are increasingly recognized causes of febrile illness in the tropics with substantive morbidity and mortality [ 3 ]. Despite the increasing recognition of infections by Rickettsia and Orientia species in the Rickettsiaceae family, only limited study of human infection by Anaplasmataceae has been conducted in tropical regions [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. This sustained lack of progress occurs despite clear evidence of the presence of Anaplasma , Ehrlichia , and Neorickettsia species in vectors and as agents of disease in wild and domestic animals.…”

Section: Introductionmentioning

confidence: 99%

Development and Clinical Validation of a Multiplex Real-Time Quantitative PCR Assay for Human Infection by Anaplasma phagocytophilum and Ehrlichia chaffeensis

Reller

Dumler

2018

TropicalMed

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Background: Human granulocytic anaplasmosis (HGA), caused by Anaplasma phagocytophilum, and human monocytic ehrlichiosis (HME), caused by Ehrlichia chaffeensis, often present as undifferentiated fever but are not treated by typical empiric regimens for acute febrile illness. Their role as agents of vector-borne febrile disease in tropical regions is more poorly studied than for other rickettsial infections. Limitations in diagnosis have impaired epidemiologic and clinical research and needless morbidity and mortality occur due to untreated illness. Methods: We designed and clinically validated a multiplex real-time quantitative PCR assay for Anaplasma phagocytophilum and Ehrlichia chaffeensis using samples confirmed by multiple gold-standard methods. Results: Clinical sensitivity and specificity for A. phagocytophilum were 100% (39/39) and 100% (143/143), respectively, and for E. chaffeensis 95% (20/21) and 99% (159/161), respectively. Conclusions: These assays could support early diagnosis and treatment as well as the high-throughput testing required for large epidemiologic studies.

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Section: Introductionmentioning

confidence: 99%

Development and Clinical Validation of a Multiplex Real-Time Quantitative PCR Assay for Human Infection by Anaplasma phagocytophilum and Ehrlichia chaffeensis

Reller

Dumler

2018

TropicalMed

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“…These animals may be important in the cycle of agents transmitted by ticks (Guimarães et al, 2018;Melo et al, 2016). Serological tests on human biological samples detected seroreactivity for Ehrlichia in Brazil (Bezerra et al, 2017).…”

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confidence: 99%

Molecular diagnostic for a screening investigation method of tick-borne pathogens in Didelphis albiventris road-killed in north of Paraná, Brazil

et al. 2023

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The synanthropization of wild animals puts public health at risk by promoting the circulation of zoonotic agents, found naturally in the wild, in the anthropic environment. The objective of this work was to carry out screening by molecular detection of pathogens of the Anaplasmatacea family in Didelphis albiventris, a specie characterized as having a synanthropic habit. Opossums that were dead (n = 25) after being road-killed were collected in the North of Paraná state, southern Brazil during the 2016 and 2018 years, through active search. A questionnaire was filled out with information about the animal and collected place. Biological samples of spleen and liver were collected. The genetic material extracted from the spleen and liver was submitted to molecular diagnosis through PCR for amplification of dsb of Ehrlichia and 16S genes for the other agents of the Anaplasmataceae family. One animal was positive for the genus Ehrlichia in semi-nested PCR for amplification of the 349 bp fragment of the dsb gene in extracted from the liver samples. In PCR for the 16S target no animal was positive. These are preliminary results that reinforce the circulation of Ehrlichia in opossums. To improve the knowledge of these agents in opossums more studies are necessary.

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“…Em relação a doenças de humanos, três espécies de Ehrlichia são reconhecidas: E. chaffeensis, causando erliquiose monocítica humana (HME), E. ewingii causando erliquiose granulocítica humana (HGE) (OLANO e WALKER., 2002), e recentemente Candidatus Neoehrlichia mikurensis também foi associada como agente de doenças em humanos na Europa e Ásia (PORTILLO et al, 2018). No Brasil, há relatos de seres humanos positivos em sorodiagnóstico (CALIC et al, 2004;SPOLIDORIO et al, 2010;BEZERRA et al, 2017), porém não existem evidências moleculares.…”

Section: Família Anaplasmataceaeunclassified

>b<Pesquisa de hemoparasitas transmitidos por carrapatos em pequenos mamíferos nos estados de São Paulo e Mato Grosso do Sul>/b<

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Este trabalho é dedicado a Danilo Gonçalves Saraiva (in memorian), jovem pesquisador e idealizador inicial desse projeto, que teve sua partida precoce, mas de alguma maneira esteve presente durante cada etapa dessa jornada. AGRADECIMENTOS Esse trabalho só pode ser realizado pelo esforço de uma equipe muito bem estruturada, então o agradecimento vai a todos os participantes do projeto temático, desde pesquisadores, alunos e demais funcionários. Agradeço com muita honra a todos os animais que de alguma maneira se doaram na participação desse projeto. Agradeço a todos os funcionários do VPS, por serem sempre tão solícitos em nos apoiar nos mais diversos desafios. Em especial ao Renato e Márcio, por todo apoio laboratorial, Pedrinho por sua gentileza e carinho com os animais do biotério, e ao Danival por ser sempre tão generoso e não medir esforços em nos ajudar nas burocracias. Agradeço a todos os colegas e amigos, essa jornada durou bons anos e contar com o apoio de todos foi imprescindível. Agradeço especialmente ao apoio de Amália, Felipe K, Herbert e Piauí no inicio do processo. A Carolina que acompanhou o início e o fim, pela parceria, pela ajuda desde o primero até o último dia, risadas, campos, perrengues e desabafos. Enfim, por ser a referência que ela é, rs.

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Seropositivity for Rickettsia spp. and Ehrlichia spp. in the human population of Mato Grosso, Central-Western Brazil

Cited by 8 publications

References 16 publications

Development and Clinical Validation of a Multiplex Real-Time Quantitative PCR Assay for Human Infection by Anaplasma phagocytophilum and Ehrlichia chaffeensis

Development and Clinical Validation of a Multiplex Real-Time Quantitative PCR Assay for Human Infection by Anaplasma phagocytophilum and Ehrlichia chaffeensis

Molecular diagnostic for a screening investigation method of tick-borne pathogens in Didelphis albiventris road-killed in north of Paraná, Brazil

>b<Pesquisa de hemoparasitas transmitidos por carrapatos em pequenos mamíferos nos estados de São Paulo e Mato Grosso do Sul>/b<

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