First report of a blaNDM-resistant gene in a Klebsiella aerogenes clinical isolate from Brazil

Soares, Cynthia Regina Pedrosa; Júnior, Jorge Belém Oliveira; Firmo, Elza Ferreira

doi:10.1590/0037-8682-0262-2020

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…Em P. mirabilis e S. marcescens foi descrito a associação de blaNDM-1 e blaKPC-2 em Pernambuco, Brasil (Firmo et al, 2020). O gene blaNDM foi detectado em um isolado de Klebsiella aerogenes pela primeira vez como citado (Soares et al, 2020) e o gene blaSPM. Os genes blaKPC, blaVIM e blaNDM foram os mais comuns relatados no estudo, assim como os genes blaKPC e blaNDM foram os mais comuns na China (Wang et al, 2018).…”

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Epidemiologia molecular de bacilos Gram-negativos multidroga resistente produtores de carbapenemases isoladas de diferentes sítios de infecção

Soares

Silva

Júnior

et al. 2021

RSD

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A resistência a carbapenemases em bacilos gram-negativas (BGN) tornou-se um problema mundial, elevando a taxa de morbidade e mortalidade. O método automatizado foi utilizado para identificação e suscetibilidade dos isolados. E o método molecular foi aplicado na detecção de bacilos gram-negativas (BGN) produtoras de carbapenemase. Um total de 205 BGN foram recuperados a partir de diferentes amostras clínicas, resistentes a carbapenemases. Os genes de resistência blaKPC (57,5%), blaVIM (30,2%), blaGES (17%), blaNDM (15%) e blaSPM (2,4%) foram recuperados a partir de isolados clínicos. O gene blaIMP não foi detectado em nenhum dos isolados. É preocupante que Pseudomonas aeruginosa e Acinetobacter baumannii representem a maioria dos isolados (61,6%) recuperados de infecções de diferentes sítios, com alta resistência a carbapenêmicos, carregando simultaneamente os genes blaKPC, blaGES e blaVIM, assim como encontrados também em Enterobactericeae. A carbapenemase mais prevalente foi blaKPC e blaVIM, seguida de blaNDM dentre os isolados multidroga resistentes. Esses resultados são um ameaça à saúde pública, configurando altas taxas de resistência, limitando as opções terapêuticas.

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Section: Discussionunclassified

Epidemiologia molecular de bacilos Gram-negativos multidroga resistente produtores de carbapenemases isoladas de diferentes sítios de infecção

Soares

Silva

Júnior

et al. 2021

RSD

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In-depth analysis of Klebsiella aerogenes resistome, virulome and plasmidome worldwide

Morgado,

Fonseca,

Freitas

et al. 2024

Sci Rep

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Klebsiella aerogenes is an emergent pathogen associated with outbreaks of carbapenem-resistant strains. To date, studies focusing on K. aerogenes have been small-scale and/or geographically restricted. Here, we analyzed the epidemiology, resistome, virulome, and plasmidome of this species based on 561 genomes, spanning all continents. Furthermore, we sequenced four new strains from Brazil (mostly from the Amazon region). Dozens of STs occur worldwide, but the pandemic clones ST93 and ST4 have prevailed in several countries. Almost all genomes were clinical, however, most of them did not carry ESBL or carbapenemases, instead, they carried chromosomal alterations (omp36, ampD, ampG, ampR) associated with resistance to β-lactams. Integrons were also identified, presenting gene cassettes not yet reported in this species (blaIMP, blaVIM, blaGES). Considering the virulence loci, the yersiniabactin and colibactin operons were found in the ICEKp10 element, which is disseminated in genomes of several STs, as well as an incomplete salmochelin cluster. In contrast, the aerobactin hypervirulence trait was observed only in one ST432 genome. Plasmids were common, mainly from the ColRNAI replicon, with some carrying resistance genes (mcr, blaTEM, blaNDM, blaIMP, blaKPC, blaVIM) and virulence genes (EAST1, senB). Interestingly, 172 genomes of different STs presented putative plasmids containing the colicin gene.

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In-depth analysis ofKlebsiella aerogenesresistome, virulome and mobilome worldwide

Morgado,

Fonseca,

Freitas

et al. 2023

Preprint

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Klebsiella aerogenes is an emergent pathogen associated with outbreaks of carbapenem-resistant strains. To date, studies focusing on K. aerogenes have been small-scale and/or geographically restricted. Here, we analyzed the epidemiology, resistome, virulome, and mobilome of this species based on 561 genomes, spanning all continents. Furthermore, we sequenced four new strains from Brazil (mostly from the Amazon region). Dozens of STs occur worldwide, but the pandemic clones ST93 and ST4 have prevailed in several countries. Almost all genomes were clinical, however, most of them did not carry ESBL or carbapenemases, instead, they carried chromosomal alterations (omp36, ampD, ampG, ampR) associated with resistance to β-lactams. Integrons were also identified, presenting gene cassettes not yet reported in this species (blaIMP, blaVIM, blaGES). Considering the virulence loci, the yersiniabactin and colibactin operons were found in the ICEKp10 element, which is disseminated in genomes of several STs, as well as an incomplete salmochelin cluster. In contrast, the aerobactin hypervirulence trait was observed only in one ST432 genome. Plasmids were common, mainly from the ColRNAI replicon, with some carrying resistance genes (mcr, blaTEM, blaNDM, blaIMP, blaKPC, blaVIM) and virulence genes (EAST1, senB). Interestingly, plasmids containing the colicin gene occurred in hundreds of genomes of different STs.

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First report of a blaNDM-resistant gene in a Klebsiella aerogenes clinical isolate from Brazil

Cited by 5 publications

References 13 publications

Epidemiologia molecular de bacilos Gram-negativos multidroga resistente produtores de carbapenemases isoladas de diferentes sítios de infecção

Epidemiologia molecular de bacilos Gram-negativos multidroga resistente produtores de carbapenemases isoladas de diferentes sítios de infecção

In-depth analysis of Klebsiella aerogenes resistome, virulome and plasmidome worldwide

In-depth analysis ofKlebsiella aerogenesresistome, virulome and mobilome worldwide

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