Seroepidemiological aspects of human infection by Strongyloides stercoralis in Alfenas, southern Minas Gerais, Brazil

Abstract

Introduction: In most Strongyloides stercoralis infected individuals, nematoidosis occurs asymptomatically, but in immunocompromised patients, it can cause hyperinfection. Serological techniques seem to be a good alternative for detecting this parasite. Methods: The frequency of seropositivity for strongyloidiasis in Alfenas, MG, was estimated using the enzyme linked immunosorbent assay on blood samples, between May and August of 2015. Results: Out of 258 samples tested, 53.9% were positive, and the frequency of seropositive individuals was higher in the peripheral districts of the municipality. Conclusions: The results indicate high seropositivity rates for strongyloidiasis among the residents of Alfenas city.

Evaluation of larval surface antigens from infective larvae of Strongyloides venezuelensis for the serodiagnosis of human strongyloidiasis

“…In order to increase the sensitivity of the diagnosis, different immunoserological tests were developed and applied for the serodiagnosis of Strongyloides infection. Among them, enzyme-linked immunosorbent assay (ELISA) is the most widely used test for detecting IgG antibodies whose main advantages are its high sensitivity and great utility in seroepidemiological studies 5 , 6 . Most of these ELISAs use antigenic extracts from other Strongyloides species, such as S. ratti and S. venezuelensis , due to limitations in obtaining antigens from S. stercoralis 4 , 7 .…”

Diagnostic accuracy of somatic and excretory−secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

“…For these reasons, detection of antibodies by immunoserological methods were developed using antigenic preparations from Strongyloides species. These tests include indirect immunofluorescence assay, gelatin particle agglutination, enzyme-linked immunosorbent assay (ELISA) and western-blot (Requena-Méndez et al, 2013), but the most widely used test is the ELISA whose main advantages are its high sensitivity and its usefulness in seroepidemiological studies (Nunes et al, 2018;Casado et al, 2019). However, due to limitations in obtaining antigens from S. stercoralis, other species such as Strongyloides ratti and S. venezuelensis have been used as a good heterologous source of antigens to develop these mentioned immunoassays (Kalantari et al, 2020).…”