Antifungal susceptibility and distribution of Candida spp. isolates from the University Hospital in the municipality of Dourados, State of Mato Grosso do Sul, Brazil

Almeida, Adriana Araújo de; Shinobu-Mesquita, Cristiane Suemi; Svidzinski, Terezinha Inez Estivalet; Oliveira, Kelly Mari Pires de

doi:10.1590/0037-8682-0074-2012

Cited by 25 publications

(24 citation statements)

References 30 publications

(44 reference statements)

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“…In addition, C. glabrata is resistant to some antifungal drugs, particularly azoles, thereby making clinical treatment difficult. [7][8][9] Although C.…”

Section: Introductionmentioning

confidence: 99%

Dectin-1 plays an important role in host defense against systemicCandida glabratainfection

et al. 2017

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Candida glabrata is the second most common pathogen of severe candidiasis in immunocompromised hosts, following C. albicans. Although C. glabrata and C. albicans belong to the same genus, they are phylogenetically distinct. C-type lectin receptors (CLRs), acting as patternrecognition receptors (PRRs), play critical roles in host defense against C. albicans infections. However, our understanding of the specific roles of CLRs in host defense against C. glabrata is limited. Here, we explored the potential roles of the C-type lectins Dectin-1 and Dectin-2 in host defense against C. glabrata. We found that both Dectin-1-deficient mice (Dectin-1 ¡/¡ ) and Dectin-2-deficient mice (Dectin-2 ¡/¡ ) are more susceptible to C. glabrata infection. Dectin-1confers host higher sensitivity for sensing C. glabrata infections, while the effect of Dectin-2 in the host defense against C. glabrata is infection dose dependent. Dectin-1 is required for host myeloid cells recognition, killing of C. glabrata, and development of subsequent Th1 and Th17 cell-mediated adaptive immune response. Significantly impaired inflammatory responses such as inflammatory cells recruitment and cytokines release that were induced by C. glabrata were manifested in Dectin-1-deficient mice. Together, our study demonstrates that Dectin-1 plays an important role in host defense against systemic Candida glabrata infections, indicating a previous unknown control mechanism for this particular type of infection in host. Our study, therefore, provides new insights into the host defense against C. glabrata.

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“…In addition, C. glabrata is resistant to some antifungal drugs, particularly azoles, thereby making clinical treatment difficult. [7][8][9] Although C.…”

Section: Introductionmentioning

confidence: 99%

Dectin-1 plays an important role in host defense against systemicCandida glabratainfection

et al. 2017

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“…Several factors increase the incidence rate of systemic candidiasis in colonized patients such as weakened immune system, mucosal and cutaneous barrier disruption, neutrophil dysfunction (quantitative or qualitative), metabolic disorders, and advanced age [2, 7]. …”

Section: Introductionmentioning

confidence: 99%

Susceptibility pattern of Candida albicans isolated from Iranian patients to antifungal agents

et al. 2016

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Background and Purpose:Candidiasis is a major fungal infection, and Candida albicans is the major cause of infections in humans. The Clinical and Laboratory Standards Institute (CLSI) developed new breakpoints for antifungal agents against C. albicans. In this multi-center study, we aimed to determine the drug susceptibility profile of C. albicans, isolated from Iranian population according to new species-specific CLSI.Materials and Methods:Clinical samples were cultured on Sabouraud dextrose agar and were incubated at room temperature for seven days. The isolates were transferred to Professor Alborzi Clinical Microbiology Research Center, Shiraz, Iran. C. albicans were identified by using API 20C AUX system. Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of amphotericin B, caspofungin, voriconazole, fluconazole, posaconazole, itraconazole, and ketoconazole, based on CLSI document M27-S4 and new breakpoints for some azoles and caspofungin.Results:Overall, 397 C. albicans were isolated from patients admitted to ten university hospitals in Iran. The MIC90 of the isolates to amphotericin B, caspofungin, voriconazole, fluconazole, posaconazole, itraconazole, and ketoconazole were 0.125, 0.125, 0.125, 1, 0.064, 0.5, and 0.125 µg/ml, and rates of resistance were 0.5%, 0.3%, 3.8%, 2.8%, and 2.5% for amphotericin B, caspofungin, voriconazole, fluconazole, and itraconazole, respectively.Conclusion:According to our data, fluconazole is the drug of choice for management of patients at risk for systemic candidiasis throughout the region, since it is cost-effective with low side effects.

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“…Decreased susceptibility to FLCZ was mainly seen against C. glabrata isolated from patients with blood stream infections, with a dose-dependent susceptibility rate of 76.5%, while the susceptibility rates of FLCZ were 100% for C. albicans and C. parapsilosis [19]. Similar results were also found for systemic candidiasis [18,20,23]. Decreases in the susceptibility of NCAC, especially of C. glabrata , resulted from resistance to FLCZ, leading to oropharyngeal Candida infections, which are more severe and more difficult to treat in HIV-infected and cancer patients [30].…”

Section: Discussionmentioning

confidence: 77%

Frequency of clinically isolated strains of oral Candida species at Kagoshima University Hospital, Japan, and their susceptibility to antifungal drugs in 2006–2007 and 2012–2013

et al. 2014

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BackgroundThe isolation frequency and susceptibility to antifungal agents of oral Candida isolates from patients with oral candidiasis (OC) were compared between studies conducted in 2006–2007 and 2012–2013.MethodsA total158 strains was isolated from 112 patients who visited Kagoshima University Hospital for the treatment of OC during the 14-month period from February 2012 and March 2013, and evaluated on the isolation frequency of each Candida strain and the susceptibility against antifungal drugs as compared to those evaluated in 2006–2007.ResultsThere was a higher frequency of xerostomia as a chief complaint and of autoimmune disease in the 2012–2013 study than in the 2006–2007 study. More than 95% of Candida isolates were C. albicans and C. glabrata. In addition, the proportion of the latter increased from 12.3% in the 2006–2007 study to 23.4% in the 2012–2013 study, while the proportion of the former decreased from 86.2% to 72.8%, respectively. C. albicans was isolated in almost all patients, while C. glabrata was only isolated concomitantly with C. albicans. Minimal inhibitory concentrations (MICs) were not significantly different between groups with a few exceptions. Candida isolates, of which MICs surpassed break points, apparently increased for miconazole and itraconazole against C. glabrata in the 2012–2013 study, but this was not statistically significant. As a result, more cases of autoimmune disease, a greater number of C. glabrata isolates, and higher resistance to azoles were seen in the 2012–2013 study than in the 2006–2007 study.ConclusionThese data indicate that with recent increases in C. glabrata infection, a causative fungus of OC, and in C. glabrata resistance to azoles, caution is needed in the selection of antifungal drugs for the treatment of OC.

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Antifungal susceptibility and distribution of Candida spp. isolates from the University Hospital in the municipality of Dourados, State of Mato Grosso do Sul, Brazil

Cited by 25 publications

References 30 publications

Dectin-1 plays an important role in host defense against systemicCandida glabratainfection

Dectin-1 plays an important role in host defense against systemicCandida glabratainfection

Susceptibility pattern of Candida albicans isolated from Iranian patients to antifungal agents

Frequency of clinically isolated strains of oral Candida species at Kagoshima University Hospital, Japan, and their susceptibility to antifungal drugs in 2006–2007 and 2012–2013

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