Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis

Conter, Carolina Cella; Neitzke-Abreu, Herintha Coeto; Pedroso, Raíssa Bocchi; Lonardoni, Maria Valdrinez Campana; Silveira, Thaís Gomes Verzignassi; Aristides, Sandra Mara Alessi

doi:10.1590/0037-8682-0052-2015

Cited by 9 publications

(5 citation statements)

References 12 publications

(12 reference statements)

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“…Polymerase chain reaction (PCR) is being increasingly used for leishmaniasis diagnosis, characterization, and identification of Leishmania species, due to the high sensitivity for the detection and identification of Leishmania species (reviewed in [47]). Quantitative PCR is a commonly used method for determining the quantity of Leishmania DNA in various tissues of laboratory animals experimentally infected with Leishmania parasites, asymptomatic humans from endemic areas, and also for testing putative reservoir hosts [18,[48][49][50]. Flow cytometry-based on the use of fluorescent probes is a relatively quick and cheap method that has been introduced to detect various protozoan parasites in the host cells [51][52][53][54].…”

Section: Discussionmentioning

confidence: 99%

Central Asian Rodents as Model Animals for Leishmania major and Leishmania donovani Research

et al. 2020

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The clinical manifestation of leishmaniases depends on parasite species, host genetic background, and immune response. Manifestations of human leishmaniases are highly variable, ranging from self-healing skin lesions to fatal visceral disease. The scope of standard model hosts is insufficient to mimic well the wide disease spectrum, which compels the introduction of new model animals for leishmaniasis research. In this article, we study the susceptibility of three Asian rodent species (Cricetulus griseus, Lagurus lagurus, and Phodopus sungorus) to Leishmania major and L. donovani. The external manifestation of the disease, distribution, as well as load of parasites and infectiousness to natural sand fly vectors, were compared with standard models, BALB/c mice and Mesocricetus auratus. No significant differences were found in disease outcomes in animals inoculated with sand fly- or culture-derived parasites. All Asian rodent species were highly susceptible to L. major. Phodopus sungorus showed the non-healing phenotype with the progressive growth of ulcerative lesions and massive parasite loads. Lagurus lagurus and C. griseus represented the healing phenotype, the latter with high infectiousness to vectors, mimicking best the character of natural reservoir hosts. Both, L. lagurus and C. griseus were also highly susceptible to L. donovani, having wider parasite distribution and higher parasite loads and infectiousness than standard model animals.

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Section: Discussionmentioning

confidence: 99%

Central Asian Rodents as Model Animals for Leishmania major and Leishmania donovani Research

et al. 2020

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“…The qPCR is considered a fast, specific, and sensitive technique for parasite detection 24,25,26,27 . It is widely used for parasite DNA detection in diverse biological samples, including peripheral blood samples 28 . Previous studies 29,30 have shown the possibility of detecting the presence of the parasite through this type of sample (eye and saliva swabs) using molecular techniques.…”

Section: Discussionmentioning

confidence: 99%

Leishmania V. braziliensis infection in asymptomatic domestic animals within an endemic region in the Northeast of Brazil

Silva

Lima

et al. 2022

Rev. Soc. Bras. Med. Trop.

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Background: American cutaneous leishmaniasis is a commonly neglected, vector-borne tropical parasitic disease that is a major public health concern in Brazil. Leishmania (Viannia) braziliensis is the main species associated with the disease. Accurate diagnosis is based on epidemiological surveillance, clinical assessment, and laboratory testing. Leishmania (V.) braziliensis has been detected in several wild and synanthropic mammals. Their epidemiological role has not been entirely elucidated. This study aimed to assess potential L. braziliensis infections in asymptomatic domestic animals, by molecular and serological testing in endemic areas, in the metropolitan region of Recife.Methods: Blood samples and conjunctival fluids were collected from 232 animals (canids, felids, equines, and caprines) for the detection of L. braziliensis using molecular tests (conventional and real-time polymerase chain reaction [PCR and qPCR]). For immunological detection, blood samples from 115 dogs were assessed using enzyme-linked immunosorbent assay.Results: Real-time quantitative PCR showed positive results for blood and conjunctival samples in all investigated species. The results of the blood and conjunctival samples were 68.2% and 26.9% in Canis familiaris, 100% and 41.7% in Felis catus, 77.3% and 30.8% in Equus caballus/Equus asinus, and 50% and 33.3% in Capra hircus samples, respectively. Conclusions:Results from this study adds valuable information to our understanding of the role of asymptomatic domestic animals, L. braziliensis life cycle, and American cutaneous leishmaniasis in Northeast Brazil.

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“…19,20 The identification of Leishmania DNA in blood sample indicates parasite dissemination. 21 Diagnostic tests for the visceral leishmaniasis form must be highly sensitive and exact because of the high risk of mortality associated with the disease. 22 Leishmania parasites circulate in the peripheral blood; in 53% of the HIV-infected patients, the parasite was detected by its appearance in the buffy coat.…”

Section: Discussionmentioning

confidence: 99%

“…DNA identification in blood of patients with cutaneous leishmaniasis has been described in some cases. 21,24 The clinical form of the disease is cutaneous leishmaniasis, but some atypical aspects of the disease that may be related to L. infantum chagasi as the causal agents of the disease and the possibility that parasite may have disseminated to other sites such as liver, spleen, and bowel despite of parasites have not been found in bone narrow.…”

Section: Discussionmentioning

confidence: 99%

Case Report: Cutaneous Leishmaniasis in a Rheumatoid Arthritis Patient Receiving Methotrexate

Amato

Souza

Franco

et al. 2022

The American Journal of Tropical Medicine and Hygiene

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The immunosuppressive effect of methotrexate has rarely been associated with reactivation of cutaneous leishmaniasis. Here we present a case of a cutaneous leishmaniasis patient with atypical clinical symptoms without splenomegaly but with cutaneous manifestations after treatment of rheumatoid arthritis with methotrexate and blood recovery of the parasite. Next-generation sequencing was used to identify Leishmania infantum chagasi in the patient’s blood sample.

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Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis

Cited by 9 publications

References 12 publications

Central Asian Rodents as Model Animals for Leishmania major and Leishmania donovani Research

Central Asian Rodents as Model Animals for Leishmania major and Leishmania donovani Research

Leishmania V. braziliensis infection in asymptomatic domestic animals within an endemic region in the Northeast of Brazil

Case Report: Cutaneous Leishmaniasis in a Rheumatoid Arthritis Patient Receiving Methotrexate

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