2017
DOI: 10.1590/0037-8682-0046-2017
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Abstract: Introduction:In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. Methods: Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, followed by evaluation using blood culture and polymerase chain reaction. T. cruzi isolates were genotyped using … Show more

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Cited by 9 publications
(15 citation statements)
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“…Most isolates from the patients were associated with DTU II. Two isolates from patients with cardiac and indeterminate clinical form, respectively, were also identified associated with DTU III or IV and DTU V or VI [49]. These data were consistent with previous studies showing that DTU II was associated with human infection in the state of Minas Gerais, Brazil [16,42,55].…”
Section: Discussionsupporting
confidence: 88%
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“…Most isolates from the patients were associated with DTU II. Two isolates from patients with cardiac and indeterminate clinical form, respectively, were also identified associated with DTU III or IV and DTU V or VI [49]. These data were consistent with previous studies showing that DTU II was associated with human infection in the state of Minas Gerais, Brazil [16,42,55].…”
Section: Discussionsupporting
confidence: 88%
“…In a previous study from our group, we detected 54.9% (50/91) of positive BCs, corresponding to first samples collected from the 91 patients [49]. The vast majority of data in the literature have reported BC positivity ranging from 40 to 70% [6,15,41,4952]. In the patients with two blood samples, blood culture positivity rate was the same (38.6%) for the first and second blood samples.…”
Section: Discussionmentioning
confidence: 97%
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“…Since 2000, PCR has been increasingly used as a tool to diagnose Chagas disease. However, very low levels of T. cruzi DNA and genetic differences between parasite strains may limit parasite detection from blood samples of patients with late chronic infections [6]. Despite current biotechnological advances, new generation serological and molecular methods still present sub-optimal performance with specific Chagasic populations [4].…”
mentioning
confidence: 99%
“…Contudo, cada vez mais se discute a necessidade de um método mais mecânico, preciso e sensível, desta forma, pode ser aplicada técnicas moleculares como a reação de polimerase em cadeia em tempo real (qPCR), a qual possui boas performances, recomendando-se o seu uso na rotina laboratorial ( SCHIJMAN, 2018;SEIRINGER et al, 2017;VOLPATO et al, 2017;ZINGALES, 2017 e apresentaram uma redução da carga parasitária considerável (redução da carga parasitária, com relação ao controle, em 84%, 64% e 44% para os bioterápicos 30DH sangue FA, 30RC sangue pre e 30RC sangue, respectivamente).…”
Section: Avaliação Da Carga Parasitária Em Camundongos Infectados Comunclassified