Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L.

Dias, Marcondes S.; P.V., Erasmo; Santos, Bianca Cotrim; Martins, Francielle Alline; Almeida, Pedro Marcos de; Perón, Ana Paula

doi:10.1590/0001-3765202120201772

Cited by 8 publications

(8 citation statements)

References 58 publications

(40 reference statements)

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“…Our analyzes indicated that piperine decreases cell viability upon application of the treatment, and it was even pointed out that the main biological effects of piperine in vitro occur at specific doses (75-200 µM) and incubation time between 24 and 48 hours [15]. This cytotoxicity effect is described as piperine promoting inhibition of NADH-oxidoreductase, an enzyme that stimulates cell activity and proliferation, as well as disruption of mitochondrial membrane permeability [16].…”

Section: Discussionmentioning

confidence: 74%

Molecular Aspects of Piperine in Signaling Pathways Associated with Inflammation in Head and Neck Cancer

Gusson-Zanetoni,

Cardoso,

De Sousa

et al. 2024

Preprint

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Piperine, an active plant alkaloid from black pepper (Piper nigrum), has several pharmacological effects: antioxidant, anti-inflammatory and immunomodulatory, which involves inhibiting molec-ular events associated with various stages of cancer development. The aim of this study was to investigate the molecular mechanisms of action of piperine in relation to its potential anticancer effect on head and neck cancer cells. Parameters related to neoplastic potential, analysis of cyto-kine, protein and gene expression were investigated in the head and neck cancer cell lines (HEp-2 and SCC-25) treated with piperine. The results of the tests indicated that piperine modified mor-phology, inhibited viability and the formation of cell colonies. Piperine promoted genotoxicity by triggering apoptosis and cell cycle arrest in G2/M and S. A decrease in cell migration was also observed, and decreased expression of MMP2/9 genes. Piperine also reduced the expression of inflammatory molecules (PTGS2 and PTGER4), regulated the secretion of cytokines (IF-y and IL-8) and modulated the expression of ERK and p38. These results suggest that piperine exerts anti-cancer effects on tumor cells, and could be a promising natural treatment by regulating signaling pathways associated with head and neck cancer.

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Section: Discussionmentioning

confidence: 74%

Molecular Aspects of Piperine in Signaling Pathways Associated with Inflammation in Head and Neck Cancer

Gusson-Zanetoni,

Cardoso,

De Sousa

et al. 2024

Preprint

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“…Similarly, Gömürgen (2005) also found that the mitotic index decreases with an increase in the concentration of potassium metabisulfite in onion apical meristem. Dias et al (2021) demonstrated cytotoxic effects of piperine, as a significant decrease in the mitotic index was observed. The root growth is controlled by a cell division in the active meristem zone, as well as by cell elongation, whereby these two processes represent independent events (Shishkova et al, 2007).…”

Section: Discussionmentioning

confidence: 94%

“…Our findings are in concordance to those from Gömürgen (2005), who observed a numerous chromosomal abnormalities in A. cepa meristem cells, such as residual chromosomes, anaphase and telophase bridges and multipolar anaphase cells, of which the most common were cells with anaphase bridges. One study demonstrated cyto/genotoxic effects of piperine due to significant increase in the chromosomal changes, pointing out at the same time that the lowest piperine concentration was not genotoxic and showed a protective effect, indicating its slight antimutagenic potential that apparently depends on the piperine concentration itself (Dias et al, 2021). Based on modern studies on animal cells, piperine has been found to have immunomodulating, antioxidant, anticancer and anti-inflammatory properties (Meghwal & Goswami, 2013).…”

Section: Discussionmentioning

confidence: 99%

Cyto/genotoxicity of potassium metabisulfite and antimutagenic effect of piperine

Hamzagić,

Mesic,

Eminović

2023

Regul. Mech. Biosyst.

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Potassium metabisulfite is a white crystalline powder used in wine production and food preservation. Piperine is an alkaloid of the piperidine series and it is the main ingredient of black pepper. The aim of the present study was to evaluate the cyto/genotoxic effects of potassium metabisulfite and antimutagenic effect of piperine using the Allium test. To test the cyto/genotoxicity of potassium metabisulfite, three concentrations were used: 0.5, 1.0 and 2.0 g/L. To evaluate the antimutagenic effect of piperine, the following concentrations were applied: 3.3, 6.6 and 13.3 µL/mL. Our results demonstrated that all three tested concentrations of potassium metabisulfite significantly reduced root growth. Mitotic index values significantly decreased after the treatment with all three concentrations of potassium metabisulfite. An increased frequency of apoptotic cells was observed for the two highest concentrations of potassium metabisulfite, while a statistically different frequency of necrosis was detected for all three potassium metabisulfite concentrations in comparison with control. The total number of cytotoxic markers differed between all three potassium metabisulfite concentrations and control. A significantly higher frequency of sticky chromosomes for the two highest potassium metabisulfite concentrations, as well as an increased number of chromosomal bridges for median potassium metabisulfite concentration as compared to control group was determined. A significantly higher number of binuclear cells was detected for the highest potassium metabisulfite concentration. A significant difference between the two highest potassium metabisulfite concentrations and control was established for the total number of aberrant cells. As for the results for piperine, a reduction in the roots' growth was observed between the two highest piperine concentrations and negative control. The mitotic index decreased after treatment with all three piperine concentrations in comparison with negative and positive control. A significant difference in the frequency of apoptosis between median piperine concentration and positive control was detected. An increased frequency of necrosis between the two highest piperine concentrations and both controls was determined. The total number of cytotoxicity markers increased between the highest piperine concentration and both controls. A significantly higher frequency of chromosomal bridges between the two highest piperine concentrations and negative control was observed. The total number of aberrant cells differed between two highest piperine concentrations and negative control. This study demonstrated that potassium metabisulfite manifests antiproliferative, cytotoxic and genotoxic effects. Similarly, antiproliferative properties of piperine have been observed, while its antimutagenic potential has not been demonstrated. Further studies are needed to strengthen these observations.

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“…Os estudos incluídos foram publicados em português (Frota et al, 2019), inglês (Pereira et al, 2017;Wandscheer et al, 2017;Montalvão et al, 2019;Khan;Anas;Malik, 2019;Almeida et al, 2020;Dias et al, 2021;Frâncica et al, 2021;Ferreira et al, 2022) e espanhol (López-Rojas; Becerra-Gutiérrez; Díaz-Vélez, 2019). Dois artigos (Pereira et al, 2017;Khan;Anas;Malik, 2019) apresentaram nível I de evidências e oito artigos (Wandscheer et al, 2017;Frota et al, 2019;López-Rojas;Becerra-Gutiérrez;Díaz-Vélez, 2019;Montalvão et al, 2019;Almeida et al, 2020;Dias et al, 2021;Frâncica et al, 2021;Ferreira et al, 2022) apresentaram nível II.…”

Section: Resultados E Discusãounclassified

Drogas usadas como controles positivos nos ensaios de toxicidade com Allium cepa L.: uma revisão da literatura

Oliveira,

Messias,

Filho

et al. 2024

PRW

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O Allium cepa L. é um bioindicador amplamente empregado em estudos biológicos devido à sua sensibilidade a agentes tóxicos. A confiabilidade desses ensaios é crucial e exige a validação por meio de controles positivos. Este estudo constitui-se em uma revisão integrativa da literatura, cuja busca foi realizada nas bases de dados PubMed, SciELO, BVS e LILACS e objetivou identificar as substâncias mais comumente utilizadas como controles positivos em pesquisas que empregam o teste Allium cepa L. Foram incluídos artigos que respondessem à questão norteadora: "Quais são as principais substâncias utilizadas como controles positivos nos ensaios de toxicidade com Allium cepa L.?". Dez estudos foram incluídos, destacando substâncias como Metanossulfonato de metila, Azida Sódica, Colchicina e outras. Os resultados revelaram variados danos celulares induzidos por diferentes substâncias. A interpretação precisa desses ensaios deve considerar nuances, incluindo a variedade específica de cebola e as concentrações das substâncias. Este estudo enfatiza a importância dessas informações para que pesquisas adicionais e a aplicação mais precisar do teste Allium cepa L. na avaliação da toxicidade.

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Cytogenotoxicity and protective effect of piperine and capsaicin on meristematic cells of Allium cepa L.

Cited by 8 publications

References 58 publications

Molecular Aspects of Piperine in Signaling Pathways Associated with Inflammation in Head and Neck Cancer

Molecular Aspects of Piperine in Signaling Pathways Associated with Inflammation in Head and Neck Cancer

Cyto/genotoxicity of potassium metabisulfite and antimutagenic effect of piperine

Drogas usadas como controles positivos nos ensaios de toxicidade com Allium cepa L.: uma revisão da literatura

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