The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2

Abstract

The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA 2 . M. indica extract, dose dependently inhibited the GIA sPLA 2 (NN-XIa-PLA 2 ) activity with an IC 50 value 8.1 μg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 μg/ ml concentration and at various concentrations (0-50 μg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA sPLA 2 . Furthermore, the inhibition was irreversible as evidenced from binding studies. It is observed that the aqueous extract of M. indica effectively inhibits sPLA 2 and it is associated infl ammatory activities, which substantiate their anti-infl ammatory properties. The mode of inhibition could be due to direct interaction of components present in the extract, with sPLA 2 enzyme. Further studies on understanding the principal constituents, responsible for the anti-infl ammatory activity would be interesting to develop this into potent anti-infl ammatory agent.

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“…Several studies showed that phytochemicals contained in mango play an anti-inflammatory role in several chronic pathological disorders associated with inflammatory responses [38,39]. Inflammatory bowel diseases, primarily including ulcerative colitis, are disorders that are characterised by chronic inflammation and mucosal damage in the large intestine.…”

Nutritional Composition, Anti-Diabetic Properties and Identification of Active Compounds Using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. Peel and Seed Kernel

“…Mangifera species is a tropical plant that is proven to have pharmacological properties such as anti-diabetic [13], anti-cancer [14], anti-inflammatory [15], and the ability to attenuate kidney damage [16]. The mango fruit ( Mangifera indica L.) and its wastes, is one of the extensively studied Mangifera species, owing to abundant nutrients and phytochemicals that benefits human health [17].…”

A Comprehensive Review on Natural Products and Anti-Inflammatory Activity

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