Propagação in vitro de cultivares de alpinia em diferentes fontes de luz

Abstract

The light source in tissue culture is often one of the punctual factors for the adequacy of plant production. For this, light emitting diodes (LEDs) have been used in order to increase the growth and development of the plants propagated in vitro. The objective was to evaluate the in vitro propagation of cultivars of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ in two different light sources (fluorescent or LEDs tubes). The experiment was conducted in a completely randomized design, factorial scheme (2x2), totaling four treatments with ten replicates, and the experimental unit composed of one flask containing five explants. After 55 days, white LED and the ‘Red Ginger’ provided the highest number of shoots. For the number of leaves, the cultivar ‘Red Ginger’ and white LED lamps provided superior results. For shoot length (cm), the cultivar ‘Red Ginger’ kept under fluorescent lamps was superior, and ‘Pink Ginger’ presented superiority when cultivated under LED lights. Thus, even with differentiated responses among genotypes, the use of white LED lamps in the in vitro propagation of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ cultivars is suggested.

Impact of monochromatic lights on the in vitro development of Cattleya walkeriana and effects on acclimatization

“…Seedlings of the Brazilian orchid Cattleya lundii (formerly Microlaelia lundii) showed lower numbers of sprouts when exposed to red and blue LED (Favetta et al, 2017). However, in in vitro propagation of Alpinia cultivars, exposure to white LED for Red Ginger cultivar provided a higher number of sprouts (Pinheiro et al, 2019). This variation in the formation of sprouts between species and cultivars was also reported in the in vitro banana crop, where Rocha et al (2017) evaluated the use of fluorescent lights and LEDs and demonstrated that the use of LED helps sprout development with variations between cultivars.…”

“…For ornamental plants, information on how the monochromatic light lengths provided by LEDs affect micropropagation are concentrated in the in vitro multiplication and rooting. Different light lengths, many in combination with other factors relevant to tissue culture, have been studied on different ornamental plants (Miler et al, 2019), such as Anthurium (Anthurium andreanum) (Martínez-Estrada et al, 2016), Oncidium tigrinum and Laelia autumnalis (Murillo-Talavera et al, 2016), Microlaelia lundii (Favetta et al, 2017), red ginger (Alpinia purpurata) (Pinheiro et al, 2019), Phalaenopsis amabilisalba Blume (Massaro et al, 2019), Moluccella laevis (Zielińska et al, 2020), Gerbera (Gerbera jamesonii) (Cioć et al, 2021), Pteris aspericaulis var. tricolor (Yu et al, 2021), andBrassavola nodosa (Vendrame et al, 2022).…”