2017
DOI: 10.1186/s13048-016-0296-x
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: BackgroundA standard histomorphometric approach has been used for nearly 40 years that identifies atretic (e.g., dying) follicles by counting the number of pyknotic granulosa cells (GC) in the largest follicle cross-section. This method holds that if one pyknotic granulosa nucleus is seen in the largest cross section of a primary follicle, or three pyknotic cells are found in a larger follicle, it should be categorized as atretic. Many studies have used these criteria to estimate the fraction of atretic follic… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

2
23
0

Year Published

2017
2017
2023
2023

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 28 publications
(25 citation statements)
references
References 32 publications
2
23
0
Order By: Relevance
“…Slides were deparaffinized and stained with hematoxylin and eosin using standard techniques to allow for follicle counts across 5–6 cross sections taken from different locations across each ovary. Primordial (one layer of squamous granulosa cells), primary follicles (one layer of cuboidal granulosa cells), and secondary follicles (two or more layers of cuboidal granulosa cells) were quantified along with hypertrophied granulosa cell clusters and atretic/misshapen/irregular follicles (Uslu et al, ); no antral follicles were identified, and corpora lutea were only sporadically noted.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Slides were deparaffinized and stained with hematoxylin and eosin using standard techniques to allow for follicle counts across 5–6 cross sections taken from different locations across each ovary. Primordial (one layer of squamous granulosa cells), primary follicles (one layer of cuboidal granulosa cells), and secondary follicles (two or more layers of cuboidal granulosa cells) were quantified along with hypertrophied granulosa cell clusters and atretic/misshapen/irregular follicles (Uslu et al, ); no antral follicles were identified, and corpora lutea were only sporadically noted.…”
Section: Methodsmentioning
confidence: 99%
“…were quantified along with hypertrophied granulosa cell clusters and atretic/misshapen/irregular follicles (Uslu et al, 2017); no antral follicles were identified, and corpora lutea were only sporadically noted.…”
Section: Histological Processingmentioning
confidence: 99%
“…Only follicles with clearly stained oocyte nuclei were counted to prevent recounting of the same follicle. If the oocyte was of degenerate appearance, or was fragmenting, the follicle was scored as atretic (Johnson et al 2004, Uslu et al 2017. For antral follicular counting, every 5th section was counted.…”
Section: Follicle Counting and Diameter Measurementioning
confidence: 99%
“…A follicle that was roughly round in appearance with an intact, non-fragmenting oocyte was scored as intact antral follicle. A follicle that either contained a fragmenting oocyte, or, was misshapen (e.g., is not subjectively round), was scored atretic antral follicles (Uslu et al 2017). Early antral follicles have emerging antral spaces, whilst later antral follicles possessed a clearly defined antral space (Johnson et al 2004, Myers et al 2004.…”
Section: Follicle Counting and Diameter Measurementioning
confidence: 99%
“…Induction of ovulation would expand the number of healthy follicles, and extracting oocytes is one of the most important reproductive tools that can be used to produce embryo in laboratory from eggs and sperm. 5,6 Maturation and resultant release of oocytes from the ovary are directly influenced by secreted hormones from the anterior pituitary specifically the effects of LH and FSH on the ovary. Gonadotropins are commonly used to stimulate the ovary and increase ovulation induction in human and animal fertilization programs.…”
Section: Introductionmentioning
confidence: 99%