Transcriptomic and metabolic responses of Calotropis procera to salt and drought stress

Mutwakil, Mohammed Z.; Hajrah, Nahid H.; Atef, Ahmed; Edris, Sherif; Sabir, Mernan J.; Al-Ghamdi, Areej K.; Sabir, Meshaal J.; Nelson, Charlotte; Makki, Rania M.; Ali, Haytham A.; El-Domyati, Fotouh M.; Al-Hajar, Abdulrahman S. M.; Gloaguen, Yoann; Al-Zahrani, Hassan S.; Sabir, Jamal S. M.; Jansen, Robert K.; Bahieldin, Ahmed; Hall, Neil

doi:10.1186/s12870-017-1155-7

Cited by 31 publications

(14 citation statements)

References 47 publications

(41 reference statements)

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“…It should be noted that we did observe a high accumulation of L-proline in the ripe LR fruit. However, a downregulation of pip in response to drought stress was also observed in Bombax ceiba 36 , and proline accumulation was triggered in Calotropis procera in response to salt stress, but not drought stress 56 , which indicates that this discrepancy may not be mere molecular noise, and might deserve further investigation.…”

Section: Discussionmentioning

confidence: 90%

Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening

Zhao

Yin

et al. 2020

Sci Rep

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Red wolfberry (or goji berry, Lycium barbarum; LB) is an important agricultural product with a high content of pharmacologically important secondary metabolites such as phenylpropanoids. A close relative, black wolfberry (L. ruthenicum; LR), endemic to the salinized deserts of northwestern china, is used only locally. The two fruits exhibit many morphological and phytochemical differences, but genetic mechanisms underlying them remain poorly explored. in order to identify the genes of interest for further studies, we studied transcriptomic (Illumina HiSeq) and metabolomic (LC-MS) profiles of the two fruits during five developmental stages (young to ripe). As expected, we identified much higher numbers of significantly differentially regulated genes (DEGs) than metabolites. The highest numbers were identified in pairwise comparisons including the first stage for both species, but total numbers were consistently somewhat lower for the LR. The number of differentially regulated metabolites in pairwise comparisons of developmental stages varied from 66 (stages 3 vs 4) to 133 (stages 2 vs 5) in both species. We identified a number of genes (e.g. AAT1, metE, pip) and metabolites (e.g. rutin, raffinose, galactinol, trehalose, citrulline and DL-arginine) that may be of interest to future functional studies of stress adaptation in plants. As LB is also highly suitable for combating soil desertification and alleviating soil salinity/alkalinity/pollution, its potential for human use may be much wider than its current, highly localized, relevance.Despite their close phylogenetic relationship, the fruits of these two species exhibit distinct phenotypic profiles of during all developmental stages, including their shape, size, colour, taste, nutritional value and pharmacological properties 3,4,11 . As opposed to the red and elongated mature red wolfberry, black wolfberry is dark-purple or black, round, and smaller. It is also known that metabolic phenotypes of these two fruits differ significantly, particularly in the content of fatty acids, phenols and antioxidant capacities, which are much higher in black wolfberry, while the content of carotenoids, sugars, amino acids and osmolytes is higher in the red wolfberry 3,4 .Fruit ripening is a complex developmental process, coordinated by a network of interacting genes and signalling pathways 12 , so genetic mechanisms underpinning these phenotypic differences remain only partially understood. The objective of this study was to contribute to our understanding of the complexity of ripening processes of these two fruits in different environments. To achieve this, we collected L. barbarum and L. ruthenicum fruits at five developmental stages, from young to ripe fruit, and sequenced their transcriptomes and metabolomes. These data shall help us better understand genetic underpinnings of both within-and between-species phenotypic differences that fruits of these two species exhibit during their respective ripening processes. Materials and methodsSample collection. Fruits were collected ...

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Section: Discussionmentioning

confidence: 90%

Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening

Zhao

Yin

et al. 2020

Sci Rep

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“…According to previous reports that the heavy metal integrates genes which changed the nature of secondary metabolites (Nasim and Dhir, 2010). Drought and osmotic stress situation enhanced the level of amino acids in medicinal plant reason behind that C. procera adapted to the harsh arid environment (Mutwakil et al, 2017).…”

Section: Rootmentioning

confidence: 99%

A Review on Calotropis Procera Its Phytochemistry and Traditional Uses

Batool¹,

Hussain²,

Hameed³

et al. 2020

Big.data.Agr

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Medicinal flora have proven their significance as a source of biologically active ingredients used in folk medicine. These active molecules have therapeutic potential and a vital pool for identifying novel medicine. Medicinal plants are widely used in developing societies like in India, Pakistan, and Southern America because they are thought to be cheaper than modern medicines, most effective and readily available. They have been used for thousands of years to treat and prevent health disorders along with epidemics. Also, they utilized for flavour and for conserving food items. They play a golden role in the development of human culture. Plants produced the diversity of secondary metabolites that are responsible for the biological functions of plants used around the world. Calotropis procera is drought-resistant medicinal perennial shrub mostly found in arid to semi-arid areas. It is a major source of secondary metabolites including phenols, flavonoids, terpenoids, sugars, alkaloids, tannins cardenolides, glycoside, saponins and steroids. It has medicinal properties as hepatoprotective, antioxidant, inflammatory, antimicrobial, and antimalarial. It is vigorous used for the ailment of common diseases, i.e. fever, leprosy, eczema, diarrhea, dysentery and jaundice. This short-review about the phytochemistry of Calotropis procera and its traditional uses.

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“…The other class includes genes that serve to protect plant cells from abiotic stress. These genes are usually involved in the following functional categories: chaperones, reactive oxygen species scavenging, stabilization of membranes, osmoprotectant biosynthesis, iron homeostasis, amino acid metabolites, sucrose transporters, phloem loading, and photosynthesis [2,3,4].…”

Section: Introductionmentioning

confidence: 99%

A Gene Regulatory Network Controlled by BpERF2 and BpMYB102 in Birch under Drought Conditions

Wen

Wang

Zhang

et al. 2019

IJMS

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Gene expression profiles are powerful tools for investigating mechanisms of plant stress tolerance. Betula platyphylla (birch) is a widely distributed tree, but its drought-tolerance mechanism has been little studied. Using RNA-Seq, we identified 2917 birch genes involved in its response to drought stress. These drought-responsive genes include the late embryogenesis abundant (LEA) family, heat shock protein (HSP) family, water shortage-related and ROS-scavenging proteins, and many transcription factors (TFs). Among the drought-induced TFs, the ethylene responsive factor (ERF) and myeloblastosis oncogene (MYB) families were the most abundant. BpERF2 and BpMYB102, which were strongly induced by drought and had high transcription levels, were selected to study their regulatory networks. BpERF2 and BpMYB102 both played roles in enhancing drought tolerance in birch. Chromatin immunoprecipitation combined with qRT-PCR indicated that BpERF2 regulated genes such as those in the LEA and HSP families, while BpMYB102 regulated genes such as Pathogenesis-related Protein 1 (PRP1) and 4-Coumarate:Coenzyme A Ligase 10 (4CL10). Multiple genes were regulated by both BpERF2 and BpMYB102. We further characterized the function of some of these genes, and the genes that encode Root Primordium Defective 1 (RPD1), PRP1, 4CL10, LEA1, SOD5, and HSPs were found to be involved in drought tolerance. Therefore, our results suggest that BpERF2 and BpMYB102 serve as transcription factors that regulate a series of drought-tolerance genes in B. platyphylla to improve drought tolerance.

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Transcriptomic and metabolic responses of Calotropis procera to salt and drought stress

Cited by 31 publications

References 47 publications

Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening

Transcriptomic and metabolomic analyses of Lycium ruthenicum and Lycium barbarum fruits during ripening

A Review on Calotropis Procera Its Phytochemistry and Traditional Uses

A Gene Regulatory Network Controlled by BpERF2 and BpMYB102 in Birch under Drought Conditions

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