The Relevance of Mammalian Peroxiredoxins to the Gametogenesis, Embryogenesis, and Pregnancy Outcomes

Li, Lianqin

doi:10.1177/1933719116667217

Cited by 5 publications

(6 citation statements)

References 99 publications

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“…Despite many studies already reporting the association between specific proteins and fertility, relatively few studies are performed on the underlying mechanism of specific reproductive diseases. For instance, several studies have extensively reported that PRDXs plays a vital role in fertility through preventing oxidative stress; however, the underlying mechanisms are not fully understood [ 142 , 143 , 144 , 145 ]. Therefore, we decided to link the differentially expressed proteins to BPA-mediated reproductive diseases manually, using the Pathway Studio program without statistical analysis.…”

Section: Resultsmentioning

confidence: 99%

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Ryu

Rahman

Pang

2017

IJMS

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Bisphenol-A (BPA) is a ubiquitous endocrine-disrupting chemical. Recently, many issues have arisen surrounding the disease pathogenesis of BPA. Therefore, several studies have been conducted to investigate the proteomic biomarkers of BPA that are associated with disease processes. However, studies on identifying highly sensitive biological cell model systems in determining BPA health risk are lacking. Here, we determined suitable cell model systems and potential biomarkers for predicting BPA-mediated disease using the bioinformatics tool Pathway Studio. We compiled known BPA-mediated diseases in humans, which were categorized into five major types. Subsequently, we investigated the differentially expressed proteins following BPA exposure in several cell types, and analyzed the efficacy of altered proteins to investigate their associations with BPA-mediated diseases. Our results demonstrated that colon cancer cells (SW480), mammary gland, and Sertoli cells were highly sensitive biological model systems, because of the efficacy of predicting the majority of BPA-mediated diseases. We selected glucose-6-phosphate dehydrogenase (G6PD), cytochrome b-c1 complex subunit 1 (UQCRC1), and voltage-dependent anion-selective channel protein 2 (VDAC2) as highly sensitive biomarkers to predict BPA-mediated diseases. Furthermore, we summarized proteomic studies in spermatozoa following BPA exposure, which have recently been considered as another suitable cell type for predicting BPA-mediated diseases.

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Section: Resultsmentioning

confidence: 99%

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Ryu

Rahman

Pang

2017

IJMS

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“…Calmodulin, for example, was present in the medium of poor-quality embryos, while being linked to successful blastocoel formation in mouse embryos [ 59 ]. Furthermore, IDHC [ 60 ], PRDX2 [ 60 , 61 ] and glucose-6-phosphatase isomerase [ 62 ] were highlighted as predictors of embryo implantation, while being detected solely in the medium conditioned by poor-quality embryos. On the other hand, LDHA1, a protein responsible for lactate synthesis, was also unique to the poor-quality medium in this study and was additionally linked to human infertility, since LDHA1 concentrations were higher in decidual trophoblast of women with recurrent miscarriage compared to women with normal pregnancies [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

Raes

Wydooghe

Pavani

et al. 2023

IJMS

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While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. We hypothesize that supplementation of embryotrophic proteins to a culture medium could be the key to improve individual embryo production. In this study, proteomics screening of culture media conditioned by bovine embryos revealed cathepsin-L as being secreted by both excellent- and good-quality embryos, while being absent in the medium conditioned by poor-quality embryos. The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. Preliminary dose–response experiments pointed out 100 ng/mL as the optimal concentration of cathepsin-L in embryo culture medium. Supplementation of cathepsin-L to individual culture systems significantly improved blastocyst development and quality in terms of blastocoel formation at day 7, and the hatching ratio and apoptotic cell ratio at day 8, compared to the control. Taken together, cathepsin-L acts as an important embryotrophin by increasing embryo quality, and regulating blastulation and hatching in bovine in vitro embryo production.

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“…The deficiency of Prx does not have lethal consequences for embryogenesis. Among the six members of Prxs, Prx2, Prx3 and Prx5 influence embryogenesis [81]. Prx2 stimulates the development of the blastocyst by reducing intracellular ROS and increasing embryonic mitochondrial activity [82].…”

Section: Anti-oxidant Enzymesmentioning

confidence: 99%

Glucose-6-Phosphate Dehydrogenase, Redox Homeostasis and Embryogenesis

Chen

Tjong

Yang

et al. 2022

IJMS

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Normal embryogenesis requires complex regulation and precision, which depends on multiple mechanistic details. Defective embryogenesis can occur by various mechanisms. Maintaining redox homeostasis is of importance during embryogenesis. NADPH, as produced from the action of glucose-6-phosphate dehydrogenase (G6PD), has an important role in redox homeostasis, serving as a cofactor for glutathione reductase in the recycling of glutathione from oxidized glutathione and for NADPH oxidases and nitric oxide synthases in the generation of reactive oxygen (ROS) and nitrogen species (RNS). Oxidative stress differentially influences cell fate and embryogenesis. While low levels of stress (eustress) by ROS and RNS promote cell growth and differentiation, supra-physiological concentrations of ROS and RNS can lead to cell demise and embryonic lethality. G6PD-deficient cells and organisms have been used as models in embryogenesis for determining the role of redox signaling in regulating cell proliferation, differentiation and migration. Embryogenesis is also modulated by anti-oxidant enzymes, transcription factors, microRNAs, growth factors and signaling pathways, which are dependent on redox regulation. Crosstalk among transcription factors, microRNAs and redox signaling is essential for embryogenesis.

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The Relevance of Mammalian Peroxiredoxins to the Gametogenesis, Embryogenesis, and Pregnancy Outcomes

Cited by 5 publications

References 99 publications

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

Glucose-6-Phosphate Dehydrogenase, Redox Homeostasis and Embryogenesis

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