Modulation of mitochondrial nitric oxide synthase  and energy expenditure in rats during cold acclimation

Peralta, Jorge G.; Finocchietto, Paola; Converso, Daniela P.; Schöpfer, Francisco J.; Carreras, Marı́a Cecilia; Poderoso, Juan José

doi:10.1152/ajpheart.00785.2002

Cited by 31 publications

(36 citation statements)

References 44 publications

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“…In contrast, Nagase et al (Nagase et al, 1997) showed that incubation of L-arginine under peroxidative conditions leads to the non-enzymatic production of NO-dependent species, which are known to increase production of O 2 · -and H 2 O 2 (Navarro et al, 2005). Peralta et al (Peralta et al, 2003) reported that L-arginine/NO, by mitochondrial NOS (mtNOS) activation, in early cold acclimation increased O 2 uptake but significantly decreased it at day 24 of late cold exposure via inhibition of cytochrome c oxidase and an increase in O 2 production. Additionally, these authors emphasized that in skeletal muscle the major NOS isoform that participates in the response to cold acclimation is mtNOS, which in this tissue was described varyingly as post-translationally modified nNOS (Elfering et al, 2002) or as eNOS (Punkt et al, 2006).…”

Section: Discussionmentioning

confidence: 97%

“…Thus, the accelerated increase in MnSOD, CuZnSOD and CAT activities observed here in L-arginine-treated rats during the early acclimation to cold could be connected to improved skeletal muscle contraction and oxidative capacity by NO and, consequently, to O 2 · -and H 2 O 2 production. However, after a period of 7 (Cannon and Nedergaard, 2004) or 10 days (Peralta et al, 2003) on cold, the impact of shivering decreases and cannot account for thermogenesis (Griggio, 1982), while NST markedly increases. During prolonged exposure to cold, despite decreased shivering, skeletal muscle retains an enhanced capacity for aerobic support of energy metabolism (although, less than in shivering), i.e.…”

Section: Discussionmentioning

confidence: 99%

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Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role ofl-arginine/NO-producing pathway

Petrović

Buzadžić

Korać

et al. 2008

Journal of Experimental Biology

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SUMMARYEarly in cold acclimation (1-7·days), heat is produced by shivering, while late in cold acclimation (12-45·days), skeletal muscle contributes to thermogenesis by tissue metabolism other than contractions. Given that both thermogenic phases augment skeletal muscle aerobic power and reactive species production, we aimed in this study to examine possible changes in skeletal muscle antioxidative defence (AD) during early and late cold acclimation with special emphasis on the influence of the Larginine/nitric oxide (NO)-producing pathway on the modulation of AD in this tissue. Adult Mill Hill hybrid hooded rat males were divided into two main groups: a control group, which was kept at room temperature (22±1°C), and a group maintained at 4±1°C for 45·days. The cold-acclimated group was divided into three subgroups: untreated, L-arginine treated and N -nitro-L-arginine methyl ester (L-NAME) treated. The AD parameters were determined in the gastrocnemius muscle on day 1, 3, 7, 12, 21 and 45 of cold acclimation. The results showed an improvement of skeletal muscle AD in both early and late cold acclimation. Clear phasedependent changes were seen only in copper, zinc superoxide dismutase activity, which was increased in early cold acclimation but returned to the control level in late acclimation. In contrast, there were no phase-dependent changes in manganese superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase, the activities of which were increased during the whole cold exposure, indicating their engagement in both thermogenic phases. L-Arginine in early cold acclimation accelerated the cold-induced AD response, while in the late phase it sustained increases achieved in the early period. L-NAME affected both early and late acclimation through attenuation and a decrease in the AD response. These data strongly suggest the involvement of the L-arginine/NO pathway in the modulation of skeletal muscle AD.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role ofl-arginine/NO-producing pathway

Petrović

Buzadžić

Korać

et al. 2008

Journal of Experimental Biology

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“…The present protocol allows to compare metabolic characteristics of rats whose serum substantially contains either T 4 or T 3 . It also allows to investigate the possible synergic effects of T 3 and catecholamines, because, plasma noradrenaline levels, which are low or normal in hyperthyroidism (Stoffer et al, 1973), remarkably increase during cold exposure (Storm et al, 1981;Peralta et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Involvement of PGC-1, NRF-1, and NRF-2 in metabolic response by rat liver to hormonal and environmental signals

Venditti

Bari

Stefano

et al. 2009

Molecular and Cellular Endocrinology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 AbstractWe studied liver oxidative capacity and O 2 consumption in hypothyroid rats treated for 10 days with T 4 , or T 3 , or treated for 10 days with T 3 and exposed to cold for the last two days. The metabolic response of homogenates and mitochondria indicated that all treatments increased the synthesis of respiratory chain components, whereas only the cold induced mitochondrial proliferation. Determination of mRNA and protein expression of transcription factor activators, such as NRF-1 and NRF-2, and coactivators, such as PGC-1, showed that mRNA levels, except PGC-1 ones, were not related to aerobic capacities. Conversely, a strong correlation was found was between cytochrome oxidase activity and PGC-1 or NRF-2 protein levels.Such a correlation was not found for NRF-1. Our results strongly support the view that in rat liver PGC-1 and NRFs are responsible for the iodothyronine-induced increases in respiratory chain components, whereas their role in cold-induced mitochondrial proliferation needs to be further on clarified.

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“…It is well known that all three isoforms of NOS, endothelial (e), neuronal (n) and inducible (i), are expressed in skeletal muscle. Furthermore, Peralta et al (Peralta et al, 2003) reported that mitochondrial NOS (mtNOS) is the major isoform in skeletal muscle that participates in the response to cold acclimation. Recently, de Castro Barbosa et al (de Castro Barbosa et al, 2012) provided direct evidence of the stimulatory effect of L-arginine on glucose and lipid metabolism in skeletal muscle, which occurs via the NO/cGMP cascade.…”

Section: P<0001 Pgc-1α/ppars In Skeletal Muscle In the Coldmentioning

confidence: 99%

Regulatory role of PGC-1alpha/PPARs signaling in skeletal muscle metabolic recruitment during cold acclimation

Stančić

Buzadžić

Korać

et al. 2013

Journal of Experimental Biology

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SUMMARYThis study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4±1°C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N ω -nitro-Larginine methyl ester (L-NAME) treated. Compared with controls (22±1°C), there was an initial increase in the protein level of 5′-AMP-activated protein kinase α (day 1), followed by an increase in peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and peroxisome proliferator-activated receptors (PPARs): PPARα and PPARγ from day 1 and PPARδ from day 7 of cold acclimation. Activation of the PGC-1α/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in β-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1α/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.

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Modulation of mitochondrial nitric oxide synthase and energy expenditure in rats during cold acclimation

Cited by 31 publications

References 44 publications

Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role ofl-arginine/NO-producing pathway

Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role ofl-arginine/NO-producing pathway

Involvement of PGC-1, NRF-1, and NRF-2 in metabolic response by rat liver to hormonal and environmental signals

Regulatory role of PGC-1alpha/PPARs signaling in skeletal muscle metabolic recruitment during cold acclimation

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