2004
DOI: 10.1128/jb.186.17.5899-5905.2004
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Abstract: The Escherichia coli argU10(Ts) mutation in the argU gene, encoding the minor tRNA Arg species for the rare codons AGA and AGG, causes pleiotropic defects, including growth inhibition at high temperatures, as well as the Pin phenotype at 30°C. In the present study, we first showed that the codon selectivity and the arginineaccepting activity of the argU tRNA are both essential for complementing the temperature-sensitive growth, indicating that this defect is caused at the level of translation. An in vitro anal… Show more

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Cited by 11 publications
(17 citation statements)
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“…It is possible that the increased accumulation of rRNA precursors, due either to tRNA defects or to antibiotic treatment, has a common basis, which we speculate as the reduced production of one or more factors that have a role in ribosome biogenesis. In an analogous situation, a ts mutation in argU, the gene encoding the rare tRNA Arg4 , was reported to cause DNA replication defects, probably because the mutant strain is unable to sufficiently produce one or more factors that play a role in replication (Henson et al 1979;Garcia et al 1986;Sakamoto et al 2004).…”
Section: Discussionmentioning
confidence: 99%
“…It is possible that the increased accumulation of rRNA precursors, due either to tRNA defects or to antibiotic treatment, has a common basis, which we speculate as the reduced production of one or more factors that have a role in ribosome biogenesis. In an analogous situation, a ts mutation in argU, the gene encoding the rare tRNA Arg4 , was reported to cause DNA replication defects, probably because the mutant strain is unable to sufficiently produce one or more factors that play a role in replication (Henson et al 1979;Garcia et al 1986;Sakamoto et al 2004).…”
Section: Discussionmentioning
confidence: 99%
“…This could affect the formation of the ternary complex (EF-Tu · GTP · aa-tRNA) required for tRNA selection to the A-site codon. The G1-to-A1 change in the acceptor-stem of the same tRNA Arg mnm 5 UCU was demonstrated to impair the EF-Tu · GTP binding (Sakamoto et al 2004). The earlier mentioned G1A mutant in tRNA Arg mnm 5 UCU [argU10(Ts)] and some other tRNA mutants, such as the divE having an A10G change in the major tRNA Ser cmo 5 UGA and feeB having a C77A change in the tRNA Leu U*AG , have one common phenotype-they all induce a cell division defect (Tamura et al 1984;Garcia et al 1986;Chen et al 1991).…”
Section: Discussionmentioning
confidence: 99%
“…Observed frameshifting occurred when the arginyl-tRNA Arg mnm 5 UCU levels were decreased only by twofold. We assume that reduction down to "trace" levels of arginylated tRNA Arg mnm 5 UCU , as in the argU10(Ts) mutant (Sakamoto et al 2004), will elicit extensive frameshifting, at least at -GAA-AGA-sites. The peptidyl-tRNA Glu mnm 5 s 2 UUC , which frameshifts at the -GAA-AGA-site, "takes off" from the GAA codon and lands at the AA-A codon.…”
Section: Discussionmentioning
confidence: 99%
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