The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing
            <i>Burkholderia</i>
            Species with Capabilities of Interest for Agriculture and Bioremediation

Caballero‐Mellado, Jesús; Onofre-Lemus, Janette; Santos, Paulina Estrada‐de los; Martínez‐Aguilar, Lourdes

doi:10.1128/aem.00324-07

Cited by 214 publications

(165 citation statements)

References 85 publications

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“…A fixação biológica de nitrogênio é uma característica ainda pouco explorada na cultura do tomateiro (Mohandas, 1988;Caballero-Mellado et al, 2007), assim como a síntese e secreção de auxinas por isolados pertencentes ao gênero Agrobacterium, relatada por Tsavkelova et al (2007). O nitrogênio fixado por bactérias associativas pode ser utilizado pelas plantas de diversas formas, enquanto o AIA estimula o crescimento vegetal e o desenvolvimento do sistema radicular, atuando no alongamento, divisão e diferenciação celular (Dobbelaere et al, 2003), estimulando também a atividade da nitrogenase (Tien et al, 1979).…”

Section: Resultsunclassified

Mecanismos de controle da murcha-de-esclerócio e promoção de crescimento em tomateiro mediados por rizobactérias

Pelzer

Halfeld-Vieira

Nechet

et al. 2011

Trop. plant pathol.

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RESUMOEste trabalho teve por objetivo caracterizar os possíveis mecanismos de antagonismo responsáveis pelo biocontrole da murchade-esclerócio e os fatores envolvidos na promoção de crescimento de tomateiro por rizobactérias selecionadas. As rizobactérias foram caracterizadas por meio da utilização de fontes de carbono, perfil de ácidos graxos e sequenciamento do 16S rDNA amplificado, enquanto os mecanismos de biocontrole e promoção do crescimento analisados através de indicadores bioquímicos e bioensaios. As rizobactérias agruparam-se com os gêneros Agrobacterium e Kluyvera. Os resultados indicam que a competição por ferro e antibiose são os possíveis mecanismos responsáveis pela capacidade de biocontrole da murcha-de-esclerócio exercida por essas rizobactérias, assim como a produção de auxinas e a fixação biológica de nitrogênio podem explicar a promoção do crescimento das plantas. Também foram demonstradas limitações do controle biológico, em função da capacidade de competição depender do isolado de Sclerotium rolfsii e, da antibiose, da composição nutricional do meio de crescimento. Palavras-chave: Athelia rolfsii, Sclerotium rolfsii, antibiose, auxinas, controle biológico, fixação biológica de nitrogênio, sideróforos. ABSTRACT Control mechanisms of southern blight and growth promotion on tomato mediated by rhizobacteriaThis study was conducted to elucidate the possible mechanisms responsible for the biocontrol of southern blight and the elements involved in tomato growth promotion by selected rhizobacteria. The rhizobacteria were characterized by carbon source utilization, fatty acid profiles, and 16S rDNA sequencing, while biocontrol mechanisms were investigated using biochemical indicators and bioassays. The rhizobacteria clustered within Agrobacterium and Kluyvera. The results indicated that iron competition and antibiosis are mechanisms that explain the biocontrol capability of southern blight by rhizobacteria. In the same way, auxin production and biological nitrogen fixation can explain growth promotion. Limitations of biological control were demonstrated because the competition capability is strain-specific for Sclerotium rolfsii, and antibiosis depends on the nutritional composition of growth media.

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Section: Resultsunclassified

Mecanismos de controle da murcha-de-esclerócio e promoção de crescimento em tomateiro mediados por rizobactérias

Pelzer

Halfeld-Vieira

Nechet

et al. 2011

Trop. plant pathol.

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“…Con lo anteriormente expuesto cabe destacar la importancia que tiene esta especie de bacteria en la biorremediación de sitios contaminados con metales pesados. Por otro lado esta bacteria ha presentado la capacidad de promover crecimiento vegetal como lo demuestra CABALLERO et al, (2007) donde establecen la presencia de esta bacteria como fijadora de nitrógeno, estudiando la rizósfera y el rizoplano en cultivos de tomate en México. Ellos determinaron su capacidad de fijar nitrógeno mediante la prueba de reducción de acetileno y la detección de los genes nifH.…”

Section: Identificación De Bacterias Resistentes a CDunclassified

Resistencia a cadmio (Cd) de bacterias endófitas y bacterias rizosféricas aisladas a partir de Oriza sativa en Colombia

et al. 2017

Rev Colombiana Cienc Anim. RECIA

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The present study had as objective to evaluate in vitro the resistance of endophytic bacteria and rizospheric bacteria to different concentrations of Cadmium.This bacteria were isolated fron different tissues of commercial rice varieties and from bacteria isolated from the rhizosphere in rice plantations of the Nechí (Antioquía) and Achí (Bolivar). Plant growth promotion was evaluated in vitro by nitrogen fixation, phosphate solubilization and siderophores production of endophytic bacteria. Of each tissue isolated from rice plants was carried out isolation in culture medium for endophytic bacteria, and the soil samples were serially diluted in peptone water. Each sample was determined the population density by counting in CFU / g of tissue and morphotypes were separated by shape, color, size and appearance in culture media. Significant differences were observed for density population of bacteria with respect to tissue, with higher values in root (4x10 11 g/root), followed of the stem (3x10 10 g/etem), leaf (5x10 9 g/ leaf), flag leaf (3x10 9 g/ flag leaf) and with less density in panicle (4x10 8 g/panicle). The results of the identification with kit API were confirmed the presence of endophytic bacteria Burkholderia cepaceae and rizospheric bacteria Pseudomona fluorescens With the ability to tolerate different concentrations of Cd, fix nitrogen, solubilize phosphates and produce siderophores. Keywords:Rizospheric bacteria; rizosphere; tolerance; Cd ResumenEl presente estudio tuvo como objetivo evaluar in vitro la resistencia de bacterias endófitas y rizosféricas a diferentes concentraciones de Cadmio (Cd). Se evaluó in vitro la promoción de crecimiento vegetal mediante la fijación de nitrógeno, solubilización de fosfato y producción de sideróforos de bacterias endófitas aisladas de diferentes tejidos de variedades de arroz comerciales y de bacterias aisladas de la rizósfera en las plantaciones de arroz de los corregimientos de Nechí (Antioquía) y Achí (Bolívar). Se colectaron muestras de raíz, tallos y hojas de plantas de arroz y de muestras de suelo rizosférico. A cada muestra se les determinó la densidad poblacional por medio de conteo en UFC/ g de tejido y se separaron morfotipos por forma, color, tamaño y apariencia en medios de cultivos. Se observaron diferencias significativas para densidad poblacional de bacterias con respecto a tejido, con mayores valores en raíz (4x10 11 g/raíz), seguida de tallo (3x10 10 g/tallo), hoja (5x10 9 g/hoja), hoja bandera (3x10 9 g/hoja bandera) y menor densidad en panícula (4x10 8 g/panícula). Los resultados de la identificación con kit API20E confirmaron la presencia de la bacteria endófita Burkholderia cepaceae y la bacteria rizosférica Pseudomona fluorescens con capacidad de tolerar diferentes concentraciones de Cd, fijar nitrógeno, solubilizar fosfatos y producir sideróforos. Palabras Clave:Bacteria endófita; rizósfera; tolerancia; Cadmio

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“…Aislamiento de bacterias degradadoras de fenol: Para aislar bacterias degradadoras de fenol se usó el medio de cultivo Bushnell-Haas (BH, Bushnell, & Haas, 1941), suplementado con 500 mg / L de fenol como única fuente de carbono (Caballero-Mellado, Onofre-Lemus, Estrada-de Los Santos, & Martínez-Aguilar, 2007;Naresh, Honey, & Vaishali, 2012). El pH se ajustó a 5.0 ± 1 debido a las condiciones de acidez de los suelos de los Llanos Orientales.…”

Section: Materiales Y Métodosunclassified

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

Pardo-Díaz

Rojas‐Tapias

Roldán

et al. 2017

RBT

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Oil exploitation and the usage of its derivatives have undeniably contributed to the technological advance worldwide. This industrial activity, however, generates several by-products that can threaten environmental sustainability. Seawage, for example, can contain organic pollutants and heavy metals; therefore, its deposition must be preceded by adequate water treatment. In this study, we isolated, characterized, and molecularly identified eight bacteria on the basis of their capability to degrade phenol. First, we determined the rates of bacterial growth and phenol degradation using different concentrations of the aromatic hydrocarbon (500, 800 and 1 200 mg / L), and then used sequential statistical designs to select optimal conditions for its degradation. Results showed that all isolated strains were capable of degrading phenol as the sole carbon source; the degradation kinetics and phenol tolerance, however, widely varied among strains. We chose the strain Pseudomonas sp. Sps1 for further studies due to its remarkable tolerance and capability to degrade phenol. By using a Plackett Burman design, followed by a fractionated factorial design in which several carbon, nitrogen, and phosphorus sources were evaluated, and phenol degradation was used as the response variable, we found optimal conditions for phenol degradation by Sps1. Finally, we compared phenol degradation in seawage by the use or not of the optimal conditions established by the statistical methods. Results showed that biostimulation dramatically increased phenol degradation compared with the control. In conclusion, we found that bioaugmentation with the native bacteria Sps1 and a statistically-based biostimulation approach provided an economically and environmentally friendly alternative for the removal of pollutants from oil industry sewage.

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The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation

Cited by 214 publications

References 85 publications

Mecanismos de controle da murcha-de-esclerócio e promoção de crescimento em tomateiro mediados por rizobactérias

Mecanismos de controle da murcha-de-esclerócio e promoção de crescimento em tomateiro mediados por rizobactérias

Resistencia a cadmio (Cd) de bacterias endófitas y bacterias rizosféricas aisladas a partir de Oriza sativa en Colombia

Biodegradación de fenol en aguas tratadas de la industria petrolera para re-uso en cultivos agrícolas

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