Fluorophore-mediated, fiber-optic, multi-analyte, immunosensing system for rapid diagnosis and prognosis of cardiovascular diseases

Abstract

A prototype of a fiber-optic, multi-analyte, immunobiosensing system was developed to simultaneously quantify disease-representing biomarkers in blood plasma. This system was for simultaneous quantification of two different groups of multi-biomarkers related to cardiovascular diseases (CVD): anticoagulants (protein C, protein S, antithrombin III, and plasminogen) for deficiency diagnosis; and cardiac markers (B-type natriuretic peptide, cardiac troponin I, myoglobin, and C-reactive protein) for coronary heart disease diagnosis. As an initial effort towards the development of a disposable and easy-to-use sensing cartridge as a rapid diagnostic tool for CVD related diseases, a prototype of a flow control system was also developed to automatically perform simultaneous four-analyte quantification. Currently, the system is capable of quantifying the multiple anticoagulants in their clinically significant sensing ranges within 5 minutes, at an average signal-to-noise (S/N) ratio of 25. A simultaneous assay of the four cardiac markers can be performed within 10 min, at an average S/N ratio of 20. When this highly portable multi-analyte sensing system is completed and successfully tested for CVD patient's plasma, it can provide rapid (<10 min) and reliable diagnostic and prognostic information at a patient's bedside.

A review of fiber-optic biosensors

“…Tapered tips are used extensively in biochemical and clinical applications [16][17][18][19]. Sensing using tapered tips is usually aided by fluorescence, which is discussed in Section 2.4.2.…”

Sensing the Whole Body and Clinical Diagnostics

“…Therefore, there is an urgent need for technological advances that could allow not only to determine these protein biomarkers quantitatively above their background levels, but also to do this in a most rapid procedure to provide correct clinical decision timely. The progress must be associated with fl uorophore-mediated multi-analyte sensing systems (Tang et al 2006 ).…”

Fiber-optic Immuno-biosensor for Rapid and Accurate Detection of Nerve Growth Factor in Human Blood

“…By performing a fluorophore mediated sandwich immunoassay within the evanescent wave field on the surface of an optical fiber [12], this technology has been a successful story for rapid clinical diagnostics [13][14][15][16]. A simultaneous assay of four cardiac-specific biomarkers in blood plasma can be completed within 10 minutes to provide physicians with critical information for rapid diagnosis and prognosis of cardiovascular diseases [17]. Here we describe the development of the fiber-optic immuno-biosensing system for the quantification of NGF concentrations in blood plasma of clinical samples.…”