A single nucleotide mutation in GID1c disrupts its interaction with DELLA1 and causes a GA‐insensitive dwarf phenotype in peach

Abstract

Summary Plant stature is one important factor that affects the productivity of peach orchards. However, little is known about the molecular mechanism(s) underlying the dwarf phenotype of peach tree. Here, we report a dwarfing mechanism in the peach cv. FenHuaShouXingTao ( FHSXT ). The dwarf phenotype of ‘ FHSXT ’ was caused by shorter cell length compared to the standard cv. QiuMiHong ( QMH ). ‘ FHSXT ’ contained higher endogenous GA levels than did ‘ QMH ’ and did not response to exogenous GA treatment (internode elongation). These results indicated that ‘ FHSXT ’ is a GA ‐insensitive dwarf mutant. A dwarf phenotype‐related single nucleotide mutation in the gibberellic acid receptor GID 1 was identified in ‘ FHSXT ’ ( GID 1c S191F ), which was also cosegregated with dwarf phenotype in 30 tested cultivars. GID 1c S191F was unable to interact with the growth‐repressor DELLA 1 even in the presence of GA . ‘ FHSXT ’ accumulated a higher level of DELLA 1, the degradation of which is normally induced by its interaction with GID 1. The DELLA 1 protein level was almost undetectable in ‘ QMH ’, but not reduced in ‘ FHSXT ’ after GA 3 treatment. Our results suggested that a nonsynonymous single nucleotide mutation in GID 1c disrupts its interaction with DELLA 1 resulting in a GA ‐insensitive dwarf phenotype in peach.

Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle

“…GA2ox is responsible for irreversibly inactivating bioactive GA or is precursors via 2-β hydroxylation 54,55 . GA stimulates the outgrowth of axillary buds in peach and Jatropha curcas 56,57 . The overexpression of GA2ox1 in poplar resulted in a reduction in the contents of the main bioactive components GA1 and GA4.…”

“…LfiGRAS1 and LfiGRAS2 belong to the GRAS family. GRAS family members such as SCR and DELLA are the main factors in GA signaling and regulation, thereby regulating various aspects of plant growth and development 49,50,57 . Decreasing LfiGRAS2 transcription levels led to the malformation of new branches ( Supplementary Fig.…”

SlGID1a Is a Putative Candidate Gene for qtph1.1, a Major-Effect Quantitative Trait Locus Controlling Tomato Plant Height

“…SlGID1a might be a good target gene for the improvement of tomato plant height. GID1s play important roles in GA signaling (Sun, 2011), which usually causes significant changes in plant phenotype, especially plant height (Cantin et al, 2018;Cheng et al, 2019;Illouz-Eliaz et al, 2019). Several GID1 mutants have been utilized in agriculture.…”

“…It contains a nonsense mutation in GID1c (Hollender et al, 2016). Two alleles of non-synonymous single nucleotide mutation, GID1c S178F and GID1c S191F , have also been found in dwarf peach (Cantin et al, 2018;Cheng et al, 2019). Three GID1 genes, SlGID1a, SlGID1b1, and SlGID1b2, are encoded in tomato genome.…”

Plant Cyclophilins: Multifaceted Proteins With Versatile Roles

“…Genes contributing to dwarfness are of agronomic importance due to their potential for developing crops that are resistant to lodging under water-logging and strong wind conditions. DELLA proteins (named after conserved N-terminal D-E-L-L-A amino acid sequence) are inhibitors of stem growth and have been implicated in dwarf phenotype in Arabidopsis, B. napus and peach (Lawit et al, 2010;Zhao et al, 2017;Cheng et al, 2019). GA degrades DELLA proteins via the ubiquitinproteasome pathway to promote stem growth (Sun, 2008(Sun, , 2010.…”