1997
DOI: 10.1104/pp.113.3.873
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Abstract: The zinnia (Zinnia elegans) mesophyll cell culture tracheary element (TE) system was used to study proteinases active during developmentally programmed cell death. Substrate-impregnated gels and single-cell assays revealed high levels of proteinase activity in differentiating TEs compared with undifferentiated cultured cells and expanding leaves. Three proteinases (1 45, 28, and 24 kD) were exclusive to differentiating TEs. A fourth proteinase (59 kD), although detected in extracts from all tissues examined, w… Show more

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Cited by 74 publications
(40 citation statements)
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“…Protease synthesis and proteosome activity have been associated with secondary cell wall development (16)(17)(18), and there is evidence for budding or vesiculation of the nucleus and the cytoplasm in Zinnia and pine TEs (19,20); vesicular removal of cell contents is a prime feature of mammalian cell apoptosis. Although there is no information on specific chromatin changes in the process (2), some elements of DNA repair also occur (1).…”
mentioning
confidence: 99%
“…Protease synthesis and proteosome activity have been associated with secondary cell wall development (16)(17)(18), and there is evidence for budding or vesiculation of the nucleus and the cytoplasm in Zinnia and pine TEs (19,20); vesicular removal of cell contents is a prime feature of mammalian cell apoptosis. Although there is no information on specific chromatin changes in the process (2), some elements of DNA repair also occur (1).…”
mentioning
confidence: 99%
“…Single-cell protease assay has indicated that differentiating TEs themselves have high proteolytic activities, which suggests cellautonomous protein degradation in TEs (Beers and Freeman, 1997). Minami and Fukuda (1995) found that the Z-Phe-Arg-MCA hydrolytic activity showed a rapid increase just before the start of autolysis in Zinnia.…”
Section: Autolysismentioning
confidence: 99%
“…Recently ZCP4, which is similar to p48h-17 gene, has also been isolated from differentiating Zinnia cells, suggesting that at least two different species of cysteine protease genes are expressed in association with autolysis (Atsuishi Minami, Taku Demura and Hiroo Fukuda, unpublished results). In addition, a serine protease of 145 kD was detected specifically in differentiating TEs on substrate-impregnated gels (Beers and Freeman, 1997). An approximately 60 kD serine protease was also reported to appear preferentially in TE-inducing culture (Ye and Varner, 1996;Beers and Freeman, 1997).…”
Section: Autolysismentioning
confidence: 99%
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