2019
DOI: 10.1101/608919
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Abstract: The complex architecture of the lung parenchyma and the air-blood barrier is difficult to mimic in-vitro. Recently reported lung-on-a-chips used a thin, porous and stretchable PDMS membrane, to mimic the air-blood barrier and the rhythmic breathing motions. However, the nature, the properties and the size of this PDMS membrane differ from the extracellular matrix of the distal airways. Here, we present a second-generation lung-on-a-chip with an array of in vivolike sized alveoli and a stretchable biological me… Show more

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Cited by 22 publications
(24 citation statements)
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References 42 publications
(45 reference statements)
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“…Several lung ALI models have been described within the last year to assess both IAV and CoV infectivity, including SARS-CoV-2, each with advantages and limitations. Small airway and alveolar lung-on-chip models have been published or reported in pre-prints [29, 79-83]. In addition, upper respiratory tract models such as nasal epithelium, which express high levels of SARS-CoV-2 receptors, have also been reported as successful in vitro culture systems for SARS-CoV-2 infection [84, 85].…”
Section: Discussionmentioning
confidence: 99%
“…Several lung ALI models have been described within the last year to assess both IAV and CoV infectivity, including SARS-CoV-2, each with advantages and limitations. Small airway and alveolar lung-on-chip models have been published or reported in pre-prints [29, 79-83]. In addition, upper respiratory tract models such as nasal epithelium, which express high levels of SARS-CoV-2 receptors, have also been reported as successful in vitro culture systems for SARS-CoV-2 infection [84, 85].…”
Section: Discussionmentioning
confidence: 99%
“…The first-generation lung-on-chip systems usually mimic a single alveolus at a time and, therefore, are not able to reproduce the typical alveolar network of the lung tissue. As there is a close relationship between mechanical forces and lung functions, it is essential to generate a network of tiny alveoli that resemble the physiological organ dynamics [ 32 ]. However, the second-generation lung-on-chip devices are able to overcome this drawback as they take advantage of a thin gold mesh with an array of hexagonal pores (Alveoli) of about 260 µm in size that serves as a scaffold where an ECM-like membrane of collagen–elastin is polymerized [ 32 ].…”
Section: Scientific Advances In the Study Of Lung Anatomymentioning
confidence: 99%
“…Lung‐on‐a‐chip models have recently received great attention as they can mimic the 3D microarchitecture and microenvironment, breathing movements as well as primary physiological functions of the human lung (Gkatzis et al, 2018; Huh et al, 2010; Huh, 2015; Li et al, 2019). Although lung‐on‐a‐chip models are not directly translatable to clinical applications, they are considered a useful tool to help lung tissue engineering and regenerative medicine (Leiby et al, 2020) by serving as a small‐scale bioreactor to perform lung physiological studies (Huh et al, 2010; Sellgren et al, 2014; Stucki et al, 2015; Zamprogno et al, 2019), disease modeling (Benam et al, 2016; Felder et al, 2014, 2019; Hassell et al, 2017; Huh et al, 2010, 2012; Jain et al, 2018; Nesmith et al, 2014; Stucki et al, 2015; Xu et al, 2013; Yang et al, 2018), toxicological studies (Huh et al, 2010; Zhang et al, 2018), and drug development (Benam et al, 2016; Huh et al, 2012; Jain et al, 2018; Xu et al, 2013).…”
Section: Other Techniques and Platforms Useful For Lung Bioengineeringmentioning
confidence: 99%
“…For example, a lung‐on‐a‐chip model containing an array of stretchable alveoli mimicked the physiological dimensions using a stretchable biological membrane made of collagen and elastin. The composition, geometry, transport, and mechanical properties of the lung alveolar barrier were imitated by this advanced lung alveolar model (Zamprogno et al, 2019). Furthermore, coculturing of A549 cells, HUVECs, and human fetal lung fibroblasts on a lung‐on‐a‐chip model revealed that A549 cells could lead to endothelial cell apoptosis or death, and then the occurrence of tumor invasion (Yang et al, 2018).…”
Section: Other Techniques and Platforms Useful For Lung Bioengineeringmentioning
confidence: 99%