2020
DOI: 10.1101/2020.03.10.986562
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Abstract: Strigolactones are plant hormones and rhizosphere signalling molecules with key roles in plant development, mycorrhizal fungal symbioses, and plant parasitism. Currently, sensitive, specific, and high-throughput methods of detecting strigolactones are limited. Here, we developed genetically encoded fluorescent strigolactone biosensors based on the strigolactone receptors DAD2 from Petunia hybrida, and HTL7 from Striga hermonthica via domain insertion of circularly permuted GFP. The DAD2 biosensor exhibited los… Show more

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Cited by 6 publications
(8 citation statements)
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“…Notably, a structural analysis revealed partial collapse of the lid domain of D14 when complexed with D3 (the rice orthologue of MAX2) in the presence of GR24 (Yao et al ., 2016). Accordingly, the decrease in the melting temperature of D14 and KAI2 observed in DSF assays has been widely interpreted to represent the transition of the receptor into an activated state (Seto et al ., 2019; Chesterfield et al ., 2020).…”
Section: Introductionmentioning
confidence: 99%
“…Notably, a structural analysis revealed partial collapse of the lid domain of D14 when complexed with D3 (the rice orthologue of MAX2) in the presence of GR24 (Yao et al ., 2016). Accordingly, the decrease in the melting temperature of D14 and KAI2 observed in DSF assays has been widely interpreted to represent the transition of the receptor into an activated state (Seto et al ., 2019; Chesterfield et al ., 2020).…”
Section: Introductionmentioning
confidence: 99%
“…We expect that this toolbox will continue to be developed in the near future. For example, sensors for strigolactones have been tested in protoplasts (Samodelov et al, 2016;Chesterfield et al, 2020;Braguy et al, 2021) and are now emerging in planta (Song et al, 2021). More such sensors will certainly emerge.…”
Section: Using Quantitative Live Imaging To Understand Hormonal Processingmentioning
confidence: 99%
“…StrigoQuant was developed with the same structure as min17‐Luc, the ratiometric sensor for auxin, using SMXL6, a homolog of rice D53, as the sensor module instead of the auxin‐responsive DII. Other ratiometric SL biosensors, namely rDAD2cpGFP and rShHTL7cpGFP, incorporate circularly permutated GFP (cpGFP) with the SL receptors DAD2 from Petunia hybrida (D14 in rice) or HTL7 from Striga hermonthica (Chesterfield et al ., 2020). The cpGFP can report conformational rearrangements induced by the ligand that cause a change in fluorescence intensity.…”
Section: Strigolactonesmentioning
confidence: 99%
“…LSSmOrange was fused to the C terminus of DAD2cpGFP and ShHTL7cpGFP via a rigid linker as reference controls. These ratiometric sensors have been used in tobacco protoplasts (Chesterfield et al ., 2020).…”
Section: Strigolactonesmentioning
confidence: 99%