Hydroxyl carlactone derivatives are predominant strigolactones in <i>Arabidopsis</i>

Yoneyama, Kaori; Akiyama, Kazuo; Brewer, Philip B.; Mori, Narumi; Kawada, Miyuki; Haruta, Shinsuke; Nishiwaki, Hisashi; Yamauchi, Satoshi; Xie, Xiaonan; Umehara, Mikihisa; Beveridge, C. A.; Yoneyama, Koichi; Nomura, T.

doi:10.1101/2020.01.17.910877

Cited by 6 publications

(12 citation statements)

References 34 publications

(40 reference statements)

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“…The LGS1 gene encodes a sulfotransferase‐like protein (Gobena et al ., 2017). Since sulfotransferases generally transfer a sulfo group to a hydroxyl group of their substrates (Hirschmann et al ., 2014), it seemed possible that the substrates of LGS1 are hydroxylated CL and hydroxylated CLA (Yoneyama et al ., 2020). We also noted that 4DO, a stereoisomer of 5DS, is synthesized via 18‐OH‐CLA in rice (Fig.…”

Section: Resultsmentioning

confidence: 99%

Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum

et al. 2021

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Summary Root parasitic plants such as Striga, Orobanche, and Phelipanche spp. cause serious damage to crop production world‐wide. Deletion of the Low Germination Stimulant 1 (LGS1) gene gives a Striga‐resistance trait in sorghum (Sorghum bicolor). The LGS1 gene encodes a sulfotransferase‐like protein, but its function has not been elucidated. Since the profile of strigolactones (SLs) that induce seed germination in root parasitic plants is altered in the lgs1 mutant, LGS1 is thought to be an SL biosynthetic enzyme. In order to clarify the enzymatic function of LGS1, we looked for candidate SL substrates that accumulate in the lgs1 mutants and performed in vivo and in vitro metabolism experiments. We found the SL precursor 18‐hydroxycarlactonoic acid (18‐OH‐CLA) is a substrate for LGS1. CYP711A cytochrome P450 enzymes (SbMAX1 proteins) in sorghum produce 18‐OH‐CLA. When LGS1 and SbMAX1 coding sequences were co‐expressed in Nicotiana benthamiana with the upstream SL biosynthesis genes from sorghum, the canonical SLs 5‐deoxystrigol and 4‐deoxyorobanchol were produced. This finding showed that LGS1 in sorghum uses a sulfo group to catalyze leaving of a hydroxyl group and cyclization of 18‐OH‐CLA. A similar SL biosynthetic pathway has not been found in other plant species.

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Section: Resultsmentioning

confidence: 99%

Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum

et al. 2021

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“…The noncanonical SL biosynthetic gene LBO is present in many seed plant species and acts in the later steps after CLA synthesis (Yoneyama et al ., 2020). It is a novel branching‐associated gene originally identified in Arabidopsis (Brewer et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Arabidopsis LATERAL BRANCHING OXIDOREDUCTASE ( LBO ) encodes a 2‐oxoglutarate‐dependent dioxygenase that oxidizes MeCLA to 1′‐HO‐MeCLA to act during the final steps of noncanonical SLs biosynthesis (Brewer et al ., 2016). Although most MeCLA is converted to CLA by LBO in vitro , 1′‐HO‐MeCLA is indeed present as an endogenous compound in Arabidopsis (Yoneyama et al ., 2020). Moreover, CL and MeCLA can accumulate in the roots of Arabidopsis lbo mutants, and the morphological characterizations induced by MeCLA accumulation are intermediate between the wild‐type Ws‐4 and the max4 mutant plants.…”

Section: Introductionmentioning

confidence: 99%

“…Overexpression of LBO in Ws‐4 plants cannot reduce branching (Brewer et al ., 2016). While the 1′‐HO‐MeCLA is highly unstable and difficult to detect in plants, the enzyme activity analysis of LBO recombinant proteins in vitro suggests that the conversion of MeCLA to 1′‐HO‐MeCLA is conserved in Arabidopsis , tomato, maize, and sorghum (Yoneyama et al ., 2020). Nevertheless, it remains unclear whether LBO regulates tiller bud outgrowth or shoot architecture in monocotyledons, especially perennial grasses.…”

Section: Introductionmentioning

confidence: 99%

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LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein‐like 2, regulates tillering in switchgrass

et al. 2022

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Summary Strigolactones (SLs) play a critical role in regulating plant tiller number. LATERAL BRANCHING OXIDOREDUCTASE (LBO) encodes an important late‐acting enzyme for SL biosynthesis and regulates shoot branching in Arabidopsis. However, little is known about the function of LBO in monocots including switchgrass (Panicum virgatum L.), a dual‐purpose fodder and biofuel crop. We studied the function of PvLBO via the genetic manipulation of its expression levels in both the wild‐type and miR156 overexpressing (miR156OE) switchgrass. Co‐expression analysis, quantitative real‐time polymerase chain reaction (qRT‐PCR), transient dual luciferase assay, and chromatin immunoprecipitation‐qPCR were all used to determine the activation of PvLBO by miR156‐targeted Squamosa Promoter Binding Protein‐like 2 (PvSPL2) in regulating tillering of switchgrass. PvLBOtranscripts dramatically declined in miR156OE transgenic switchgrass, and the overexpression of PvLBO in the miR156OE transgenic line produce fewer tillers than the control. Furthermore, we found that PvSPL2 can directly bind to the promoter of PvLBO and activate its transcription, suggesting that PvLBO is a novel downstream gene of PvSPL2. We propose that PvLBO functions as an SL biosynthetic gene to mediate tillering and acts as an important downstream factor in the crosstalk between the SL biosynthetic pathway and the miR156‐SPL module in switchgrass.

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“…Recently, the direct conversion of carlactonoic acid to orobanchol without passing through 4-deoxyorobanchol has been described ( Wakabayashi et al., 2019 ). Moreover, a cytochrome P450 and a 2-oxoglutarate-dependent dioxygenase genes were identified being involved in SL synthesis in Lotus japonicus ( Mori et al., 2020 ), and hydroxyl carlactone derivatives as relevant intermediaries in SL synthesis have been identified in Arabidopsis ( Yoneyama et al., 2020 ). Despite the active research, our knowledge about the details of SL biosynthesis after carlactone is still limited ( Bouwmeester et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Strigolactones Interact With Nitric Oxide in Regulating Root System Architecture of Arabidopsis thaliana

et al. 2020

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Both nitric oxide (NO) and strigolactone (SL) are growth regulating signal components in plants; however, regarding their possible interplay our knowledge is limited. Therefore, this study aims to provide new evidence for the signal interplay between NO and SL in the formation of root system architecture using complementary pharmacological and molecular biological approaches in the model Arabidopsis thaliana grown under stressfree conditions. Deficiency of SL synthesis or signaling (max1-1 and max2-1) resulted in elevated NO and S-nitrosothiol (SNO) levels due to decreased S-nitrosoglutathione (GSNO) reductase (GSNOR) protein abundance and activity indicating that there is a signal interaction between SLs and GSNOR-regulated levels of NO/SNO. This was further supported by the down-regulation of SL biosynthetic genes (CCD7, CCD8 and MAX1) in GSNOR-deficient gsnor1-3. Based on the more pronounced sensitivity of gsnor1-3 to exogenous SL (rac-GR24, 2 µM), we suspected that functional GSNOR is needed to control NO/SNO levels during SL-induced primary root (PR) elongation. Additionally, SLs may be involved in GSNO-regulated PR shortening as suggested by the relative insensitivity of max1-1 and max2-1 mutants to exogenous GSNO (250 µM). Collectively, our results indicate a connection between SL and GSNOR-regulated NO/ SNO signals in roots of A. thaliana grown in stress-free environment. As this work used max2-1 mutant and rac-GR24 exerting unspecific effects to both SL and karrikin signaling, it cannot be ruled out that karrikins are partly responsible for the observed effects, and this issue needs further clarification in the future.

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Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis

Cited by 6 publications

References 34 publications

Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum

Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum

LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein‐like 2, regulates tillering in switchgrass

Strigolactones Interact With Nitric Oxide in Regulating Root System Architecture of Arabidopsis thaliana

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