Cross-reactivity between Enteric Adenoviruses and Adenovirus Type 4: Analysis of Epitopes by Solid-phase Immune Electron Microscopy

Svensson, Lennart; Wadell, Göran; Uhnoo, Ingrid; Johansson, Mats; Bonsdorff, Carl-Henrik von

doi:10.1099/0022-1317-64-11-2517

Cited by 20 publications

(7 citation statements)

References 12 publications

(9 reference statements)

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“…This suggestion is based on several observations that Ad 4 displays an immunological cross-reactivity on the hexon level with Ad 16 of subgenus B [25] and also on the vertex capsomer level to the other members of subgenus B. Hyperimmune sera against Ad 4 virions cross-react with exposed epitopes on the surface of virions of Ad 40, a member of subgenus F. Ad 4 [20] forms dodecons which are characteristic of members of subgenera B, D, and E only [24]. The length of Ad 4 fibre is intermediate between members of subgenera C and D [24].…”

Section: Discussionmentioning

confidence: 94%

The degree of genetic variability among adenovirus type 4 strains isolated from man and chimpanzee

Wadell

1988

Archives of Virology

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A total of 8 different genome types of adenovirus type 4 (Ad 4), Ad 4p, Ad 4p 1-3, Ad 4a, Ad 4a 1, Ad 4b, and Ad 4ch were identified among 50 selected human adenovirus isolates and 2 chimpanzee adenovirus strains using 16 restriction endonucleases Bam HI, Bcl I, Bgl I, Bgl II, Bst EII, Dra I, Eco RI, Eco RV, Hind III, Hpa I, Sal I, Sma I, Ssp I, Pst I, Xba I, and Xho I. They could be divided into three genomic clusters. Cluster 1 contained Ad 4p and Ad 4p 1-3; cluster 2 contained Ad 4a, ad 4a 1 and Ad 4b; whereas the chimpanzee Ad 4 genome type was the unique member of cluster 3. The degree of genetic variability within each cluster was minor. The genome types within one cluster display 95-99% pairwise comigrating restriction fragments (PCRF). However, the genetic space between the three clusters was large. The genome types between different clusters share only 25-46% PCRF. A comparative PCRF analysis performed with restriction endonucleases Apa I, Nar I, Nae I, Sac II, and Sma I recognizing exclusively G and C sequences and Dra I, Ssp I recognizing exclusively A and T containing sequences revealed that G and C rich regions were significantly more conserved than A and T rich regions.

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Section: Discussionmentioning

confidence: 94%

The degree of genetic variability among adenovirus type 4 strains isolated from man and chimpanzee

Wadell

1988

Archives of Virology

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“…An extensive reciprocal cross-reaction was seen between Ad40 and Ad4l by the method of solid-phase immune electron microscopy (16). There was a much closer relationship between Ad4O and Ad4l when continuous human cell cultures (Chang or Graham 293) were used in the NTs.…”

Section: Discussionmentioning

confidence: 94%

Antibody response to enteric adenovirus types 40 and 41 in sera from people in various age groups

et al. 1987

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A total of 322 single and paired serum samples from children (newborn to 10 years old), young adults (18 to 20 years old), pregnant women and their cord serum samples, and elderly people (more than 70 years old) was tested for antibodies to enteric adenovirus types 40 and 41 by neutralization test. Serum samples were also tested for antibody to the common antigen of adenovirus by enzyme immunoassay. The incidence of antibodies rose gradually through childhood. Antibodies were found in 20% of children between 1 and 6 months old and in 50% of those 37 to 48 months old. Of serum samples from young adults, 48% had antibodies. Antibodies were found in 10% of serum samples from the aged. Of patients with acute gastroenteritis, 19% showed a significant rise in antibody to adenovirus type 40 or 41 or both, and 42% of the same serum samples had a significant rise in antibody to rotavirus by enzyme immunoassay. None of the serum samples tested was negative to adenovirus common antigen. Noncultivatable adenoviruses have been demonstrated by electron microscopy in stool samples from children with diarrhea (5, 11, 13). On the basis of antigenic and DNA restriction pattern techniques, adenovirus types 40 and 41 (Ad40 and Ad4l, respectively) have been identified in stool specimens from patients with infantile gastroenteritis (4, 17, 20). Enteric adenoviruses are recognized as the second most commonly identified agent after rotavirus in stool samples of infants and young children with viral gastroenteritis (2, 10, 17). The seroepidemiology of enteric adenoviruses has been reported only by Kidd and his colleagues (9, 10). We report here the seroprevalence of neutralizing antibody to enteric adenoviruses in serum samples from various age groups in Japan.

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“…Ad4, the sole member of this group, is antigenically unique except for its bilateral SN relationship with type 16 [25, 44, 53, 65] and its interesting relationship to types 40 and 41 [54].…”

Section: Subgenus Ementioning

confidence: 99%

Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera

Hierholzer

Stone

Broderson

1991

Archives of Virology

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Summary.Reference equine antisera to all 47 serotypes of human adenoviruses presently described have been prepared and evaluated by reciprocal neutralization and hemagglutination-inhibition tests. All tests were carried to endpoint dilutions a minimum of five times in each direction to give accurate values for homologous and heterologous antibody titers. Significant cross-reactions in the horse antisera were compared to similar data obtained from rabbit antisera. Using this analysis, major antigenic relationships exist among types 12-18-31 of subgenus A, types 7-11-14 and 34-35 of subgenus B, types

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Cross-reactivity between Enteric Adenoviruses and Adenovirus Type 4: Analysis of Epitopes by Solid-phase Immune Electron Microscopy

Cited by 20 publications

References 12 publications

The degree of genetic variability among adenovirus type 4 strains isolated from man and chimpanzee

The degree of genetic variability among adenovirus type 4 strains isolated from man and chimpanzee

Antibody response to enteric adenovirus types 40 and 41 in sera from people in various age groups

Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera

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