2002
DOI: 10.1096/fj.02-0259fje
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Abstract: Fluorescence microscopy of A549 cells stained with a glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH)-specific polyclonal antibody displayed uniform staining of the peri-nuclear cytosol, with the nuclear region apparently lacking GSH staining. This discontinuous staining was confirmed in other cell types and also corroborated in A549 cells stained with the thiol-reactive dye mercury orange. The selectivity of antibody binding was confirmed by buthionine sulfoximine (BSO)-dependent inhibition of GSH synth… Show more

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Cited by 92 publications
(62 citation statements)
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“…However, when cells were confluent, at 3 and 5 days after plating, no differences between the nucleus and cytoplasm could be seen. The images obtained at 3 or 5 days in culture are similar to those reported previously by Söder-dahl et al (5) using GSH antibody and mercury orange. Thus, our results may explain the apparent discrepancies in the GSH distribution.…”
Section: Discussionsupporting
confidence: 86%
See 1 more Smart Citation
“…However, when cells were confluent, at 3 and 5 days after plating, no differences between the nucleus and cytoplasm could be seen. The images obtained at 3 or 5 days in culture are similar to those reported previously by Söder-dahl et al (5) using GSH antibody and mercury orange. Thus, our results may explain the apparent discrepancies in the GSH distribution.…”
Section: Discussionsupporting
confidence: 86%
“…They suggested that there is a distinct pool of GSH in the nucleus because in their experiments GSH was partially resistant to buthionine sulfoximine depletion compared with the cytoplasm (15). More recently, Söderdahl et al (5) showed the highest GSH staining in a perinuclear mitochondria-rich compartment and low nuclear GSH staining using mercury orange and a specific GSH antibody. Finally, Voehringer et al (12) using CMFDA (which binds in 95% to GSH, see "Experimental Procedures" and Ref.…”
Section: Discussionmentioning
confidence: 97%
“…4). Diamide did not generate significant GSSG, consistent with the known ability of diamide to yield GS-protein adducts in cells (Soderdahl et al, 2003). Since Cr(VI) did not decrease GSH levels in these cells, the oxidation of Trx1 and Trx2 cannot be explained as the result of a general oxidation of cellular thiols.…”
Section: Cr(vi) and Gsh Levelssupporting
confidence: 77%
“…7). Diamide did not generate significant GSSG, consistent with the known ability of diamide to yield GS-protein adducts in cells [48]. That GSSG was below detection limits in cells is consistent with GSSG normally comprising <2% of the total glutathione pool [49].…”
Section: Role Of Thiolssupporting
confidence: 75%