Brassinosteroid-Induced Bending of the Leaf Lamina of Dwarf Rice Seedlings: An Auxin-Mediated Phenomenon

Takeno, Kiyotoshi; Pharis, Richard P.

doi:10.1093/oxfordjournals.pcp.a076471

Cited by 115 publications

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“…This inclination was synergized by the application of IAA + BR (10). Based on these earlier findings we have developed a very sensitive dwarf rice lamina inclination assay for detection and approximate quantification of BR.…”

Section: And Discussion Comments On the Usefulness Of The Rice-laminamentioning

confidence: 86%

“…It was previously reported (10) that an inclination between the leaf sheath and lamina could occur in intact dwarf rice seedlings after application of BR. This inclination was synergized by the application of IAA + BR (10).…”

Section: And Discussion Comments On the Usefulness Of The Rice-laminamentioning

confidence: 90%

“…Preparation of dwarf rice (Oryza sativa var Tan-ginbozu, provided by Dr. M. Koshioka, National Institute of AgroEnvironental Sciences, Tsukuba, Japan) seedlings for the lamina inclination BR bioassay was described previously (10).…”

Section: Bioassaymentioning

confidence: 99%

“…This is the first evidence for the presence of BR in such material. Since the control of cambial growth in conifers apparently involves IAA (6), and BR interacts synergistically with IAA in several plant systems (1,3,8,10,16,17), the possible interaction of BR and IAA in connection with conifer cambial activity is now being investigated (RA Savidge, CHA Little, S-K Kim, RP Pharis, unpublished data).…”

Section: Identification Of Br In the Cambial Region Of Scots Pinementioning

confidence: 99%

“…Because IAA appears to play an important role in the regulation of cambial activity in woody plants (6), and BR is known to interact synergistically with IAA (1,3,8,10,16,17), we looked for BRs in scrapings ofthe cambial region of Scots pine (Pinus silvestris) harvested during the cambial growing period. In the process, we also developed a sensitive and simple dwarfrice lamina inclination bioassay based on the intact seedling assay of Takeno and Pharis (10). This assay uses dwarf rice for the detection of BR in extracts of plant tissue.…”

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Identification of Two Brassinosteroids from the Cambial Region of Scots Pine (Pinus silverstris) by Gas Chromatography-Mass Spectrometry, after Detection Using a Dwarf Rice Lamina Inclination Bioassay

Kim¹,

H²,

Little³

et al. 1990

Plant Physiol.

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A simple and improved dwarf rice (Oryza sativa var Tan-ginbozu) lamina inclination bioassay for brassinosteroids (BRs) was developed based on a previously published method (K Takeno, RP Pharis [1982] Plant Cell Physiol 23: 1275-1281). The assay used 3-day-old intact seedlings, and detection of BR was made more sensitive by synergizing the response to BR with indole-3-acetic acid (IAA). The minimum detectable amount of BR was less than 0.1 nanogram/rice plant (brassinolide equivalents). Purification steps for isolation of BR from tissue scrapings taken from the cambial region of Scots pine (Pinus silverstris) harvested during the period of rapid wood production were guided by this assay. After column chromatography (silica gel and PrepPak C18) and reversed phase Cie high performance liquid chromatography, the biologically active fractions were analyzed by gas chromatography-mass spectrometry (GC-MS) and/or GC-MS-selected ion monitoring. Two BRs, castasterone (major) and brassinolide (minor) were identified. This is the first identification of BR from the cambial region of a conifer.Brassinosteroids (BRs)4 are steroidal hormones that promote plant growth. To date, 28 naturally occurring BRs have been identified in various plant tissues (2,4,5,8,16), ranging from higher plants to a green alga ( 15). In conifers, castasterone (scheme 1, structure 2) and typhasterol (2-deoxycastasterone (scheme 1, structure 3) have been identified in pine pollen

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Section: And Discussion Comments On the Usefulness Of The Rice-laminamentioning

confidence: 86%

Section: And Discussion Comments On the Usefulness Of The Rice-laminamentioning

confidence: 90%

Section: Bioassaymentioning

confidence: 99%

Section: Identification Of Br In the Cambial Region Of Scots Pinementioning

confidence: 99%

mentioning

confidence: 99%

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Identification of Two Brassinosteroids from the Cambial Region of Scots Pine (Pinus silverstris) by Gas Chromatography-Mass Spectrometry, after Detection Using a Dwarf Rice Lamina Inclination Bioassay

Kim¹,

H²,

Little³

et al. 1990

Plant Physiol.

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Brassinosteroids

Abe

Nishikawa-Koseki

2002

Encyclopedia of Agrochemicals

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Brassinosteroids are a sixth natural plant hormone that plays an essential regulatory role in plant growth and development. As of the year 2000, over 40 different structures of brassinosteroids had been discovered. Recent advances in research on brassinosteroid‐deficient and ‐insensitive mutants from Arabidopsis, pea, and tomato, and their molecular genetic analysis, are opening new insights into the physiology. The research on biosynthesis and metabolism is also growing rapidly. Brassinosteroids are expected to be used in the future for crop production and plant protection as a new type of plant growth regulator.

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Brassinosteroids

Moore¹

1989

Biochemistry and Physiology of Plant Hormones

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Brassinosteroid-Induced Bending of the Leaf Lamina of Dwarf Rice Seedlings: An Auxin-Mediated Phenomenon

Cited by 115 publications

References 0 publications

Identification of Two Brassinosteroids from the Cambial Region of Scots Pine (Pinus silverstris) by Gas Chromatography-Mass Spectrometry, after Detection Using a Dwarf Rice Lamina Inclination Bioassay

Identification of Two Brassinosteroids from the Cambial Region of Scots Pine (Pinus silverstris) by Gas Chromatography-Mass Spectrometry, after Detection Using a Dwarf Rice Lamina Inclination Bioassay

Brassinosteroids

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