“…Y. lipolytica genes YALI0F17820g ( ylGDH1 ) and YALI0E09603g ( ylGDH2 ), predicted to lack introns and encode potential GDHs (Kersey et al, ; D. J. Sherman et al, ), were amplified by polymerase chain reaction (PCR; for specific primer sequences, see Table S3) from DNA of Y. lipolytica strain INAG34815 (Table S1). The resulting 1.4‐kb ( ylGDH1 ) and 3.0‐kb ( ylGDH2 ) PCR fragments were gel purified, blunted using a mixture of polymerase and kinase (Quick Blunting Kit, NE Biolabs, Ipswich, MA), and ligated into the dephosphorylated Sma1 site of the S. cerevisiae pBEVY‐L vector (Miller, Martinat, & Hyman, ) under the constitutive GPD/ADH1 promoter with leucine selection.…”