Abstract:Background Vitamin B12 is one of the important vitamins in human body, its store in liver and mainly can be obtained from animal resource food, so vegetarians always should have replacement as it role in cell formation and nerves as well. Decreased B12 absorption could be due to malabsorption which occurs with pancreatic insufficient and courseany surgery, intestinal disease and trophic gastritis, which increases with so that is why elder populations normally develop low absorption and decline in many organs function with time, so this study aimed to evaluate B12 status among apparently healthy elder people in Khartoum-Sudan. Material and method Apparently health elderly 100 subjects voluntarily enrolled and gave permission of blood collection and measuring of plasma level of B12 by means of chemiluminescent immunoassay (CLIA) . Hemoglobin (Hb) concentration also measured in the process by means of hematology analyzer Mindray BC300. Result The data obtained, revealed that B12 inversely correlated with age and there was no different in its levels according to gender. Conclusion So apparent healthy could be deceptive as B12 level low didn't present with obvious symptoms yet or missed, it recommended to set routine analysis for elder population include B12 and replacement for low level to avoid predicted damage due to its deficiency in the body.