1967
DOI: 10.1083/jcb.32.2.415
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Abstract: Preparations of rat-liver mitochondria catalyze the oxidation of exogenous NADH by added cytochrome c or ferricyanide by a reaction that is insensitive to the respiratory chain inhibitors, antimycin A, amytal, and rotenone, and is not coupled to phosphorylation. Experiments with tritiated NADH are described which demonstrate that this "external" pathway of NADH oxidation resembles stereochemically the NADH-cytochrome c reductase system of liver microsomes, and differs from the respiratory chain-linked NADH deh… Show more

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Cited by 2,249 publications
(608 citation statements)
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References 43 publications
(55 reference statements)
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“…The renal microsomal sample was analyzed only for specific content of CYP and specific activity of CYP1A (ethoxyresorufin O-deethylation). The activity of NADPH:CYP reductase in human hepatic and renal microsomes was measured according to Sottocasa et al 41 using cytochrome c as substrate (i.e., as NADPH: cytochrome c reductase). The concentration of NADPH:CYP reductase was estimated as described previously.…”
Section: Preparation Of Microsomes and Assaysmentioning
confidence: 99%
“…The renal microsomal sample was analyzed only for specific content of CYP and specific activity of CYP1A (ethoxyresorufin O-deethylation). The activity of NADPH:CYP reductase in human hepatic and renal microsomes was measured according to Sottocasa et al 41 using cytochrome c as substrate (i.e., as NADPH: cytochrome c reductase). The concentration of NADPH:CYP reductase was estimated as described previously.…”
Section: Preparation Of Microsomes and Assaysmentioning
confidence: 99%
“…Pi liberated in the above essays was determined as described by Martin & Doty (1949). NADPH-cytochrome c oxidoreductase activity (EC 1.6.2.4) was assayed by the method of Sottocasa et al (1967). Succinate dehydrogenase activity (EC 1.3.99.1) was assayed by the procedure of Earl & Korner (1965).…”
Section: Experimental Methodsmentioning
confidence: 99%
“…The cytochrome-b5-reductase activity was measured as described previously (27,28). Briefly, liver tissues were homogenized in ice-cold 0.25 M sucrose and 10 mM HEPESNaOH, pH 7.8 (iso-osmotic buffer, 4 ml/g of liver) with a cocktail of protease inhibitors (Roche Diagnostics, Indianapolis, IN, USA).…”
Section: Cytochrome-b5-reductase Activitymentioning
confidence: 99%
“…The protein concentration of the sample was determined by the standard Bradford assay. The enzymatic reaction was carried out in a 50 mM phosphate buffer, pH 7.5 (27,28), in the presence of 0.3 mM KCN (to inhibit oxidases) and 2 μM rotenone (to inhibit mitochondrial NADH cytochrome C reductases). The reaction was started by the addition of 0.5 mM NADH.…”
Section: Cytochrome-b5-reductase Activitymentioning
confidence: 99%