1991
DOI: 10.1083/jcb.115.3.717
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Abstract: Abstract. Tau protein plays a role in the extension and maintenance of neuronal processes through a direct association with microtubules . To characterize the nature of this association, we have synthesized a collection of tau protein fragments and studied their binding properties. The relatively weak affinity of tau protein for microtubules (ti10-' M) is concentrated in a large region containing three or four 18 amino acid repeated binding elements. These are separated by apparently flexible but less conserve… Show more

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Cited by 513 publications
(443 citation statements)
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“…The main sites affecting microtubule binding and dynamics are in the repeat domain although this domain, taken by itself, interacts only rather weakly with microtubules (Ennulat et al, 1989;Joly and Purich, 1990;Butner and Kirschner, 1991;Gustke et al, 1994). On the other hand, the domains flanking the repeats, particularly the basic and proline-rich domains, cause strong binding to microtubules Lee and Rook, 1992;Olson et al, 1995;Ookata et al, 1995); however, their phosphorylation has only a limited effect on microtubule interactions.…”
Section: Discussionmentioning
confidence: 99%
“…The main sites affecting microtubule binding and dynamics are in the repeat domain although this domain, taken by itself, interacts only rather weakly with microtubules (Ennulat et al, 1989;Joly and Purich, 1990;Butner and Kirschner, 1991;Gustke et al, 1994). On the other hand, the domains flanking the repeats, particularly the basic and proline-rich domains, cause strong binding to microtubules Lee and Rook, 1992;Olson et al, 1995;Ookata et al, 1995); however, their phosphorylation has only a limited effect on microtubule interactions.…”
Section: Discussionmentioning
confidence: 99%
“…14 The number of repeats can modulate tau-microtubule affinity. [15][16][17] Consistent with the weaker microtubule binding of three-repeat (3R) tau isoforms, only 3R tau is expressed during neurogenesis, when higher microtubule dynamics are required. 18 Phosphorylation of the repeat region and, to a lesser extent, also of the flanking regions impairs the tau-microtubule interaction and leads to tau detachment from microtubules.…”
Section: Cell Biology and Function Of Taumentioning
confidence: 99%
“…Microtubules can be prepared from brain extracts by repetitive cycles of assembly and disassembly which can be monitored conveniently by light scattering (Gaskin et al 1974;Weisenberg 1972 (Cleveland et al 1977a;Butner and Kirschner 1991;Goode and Feinstein 1994;Gustke et al 1994;Hong et al 1998;Makrides et al 2004;Sillen et al 2007). Generally, Tau mutations (i.e., those occurring in FTDP17) tend to weaken the binding to microtubules somewhat (Hong et al 1998;Barghorn et al 2000); phosphorylation can have small or major inhibitory effects, depending on the type and number of sites (e.g., phosphorylation at sites inside the repeat domain has a larger effect than at sites outside; Biernat et al 1993).…”
Section: Tau and Microtubulesmentioning
confidence: 99%