2019
DOI: 10.1080/19491034.2019.1644592
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Abstract: The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy is indispensable for resolving nuclear architecture. However, novel solutions are required for capturing cell-spec… Show more

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Cited by 31 publications
(30 citation statements)
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“…This can be viewed from two opposing perspectives. Autofluorescence can be considered problematic [2][3][4]-autofluorescent molecules may interfere with the detection of fluorescent staining, reporter molecules such as GFP (green fluorescent protein), or with immunolocalization techniques. Alternatively, autofluorescence can be used as a label-free method for detecting specific molecules using spectral fluorescence imaging techniques and hence can be considered advantageous for some experimental investigations [1,[5][6][7][8][9][10][11][12].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…This can be viewed from two opposing perspectives. Autofluorescence can be considered problematic [2][3][4]-autofluorescent molecules may interfere with the detection of fluorescent staining, reporter molecules such as GFP (green fluorescent protein), or with immunolocalization techniques. Alternatively, autofluorescence can be used as a label-free method for detecting specific molecules using spectral fluorescence imaging techniques and hence can be considered advantageous for some experimental investigations [1,[5][6][7][8][9][10][11][12].…”
Section: Introductionmentioning
confidence: 99%
“…Fluorescence emission of common autofluorescent compounds found in plants[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”
mentioning
confidence: 99%
“…Novel technical advances making use of CRISPR/Cas9 based strategies or new developments in 3C techniques together with a deeper characterization of multisubunit complexes and their functions will help to better our understanding of the organization of plant genomes and nuclear protein networks in the near future. Simultaneously, studying the interplay between different regulators with the help of emerging technologies, such as the development of imaging and imageprocessing solutions that take into account the challenges of plant systems (Dumur et al, 2019) and other cell-specific techniques, will certainly yield important new findings. Finally, while most work presented at the meeting used Arabidopsis as a model system, the fundamental mechanisms identified might in the future be applied to crop species by, for example, exploiting natural epigenetic diversity in plant breeding or induced epigenetic variation involved in stress priming and memory (Springer and Schmitz, 2017;Mozgová et al, 2019;Forestan et al, 2019).…”
Section: Discussionmentioning
confidence: 99%
“…Future investigations of the chromatin remodeler-histone chaperone interactome and comprehensive chromatin immunoprecipitation sequencing analyses will be useful for elucidating the mechanism by which plant root tips precisely respond to environmental stimuli and developmental needs. Furthermore, the available techniques for in situ chromatin studies are critical for resolving the nuclear architecture [99]. High resolution microscopy enables the three-dimensional examination of the plant nuclear architecture and the characterization of the spatial dynamics of the nuclear compartment, specifically regarding the chromatin remodeling factors and histone chaperones.…”
Section: Perspectivementioning
confidence: 99%