Use of elephant grass (<i>Pennisetum purpureum</i>) acid hydrolysate for microbial oil production by<i>Trichosporon cutaneum</i>

Chen, Xuefang; Huang, Chao; Wang, Bo; Qi, Gaoxiang; Lin, Xiaoqing; Wang, Can; Chen, Xinde

doi:10.1080/10826068.2015.1135453

Cited by 11 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has a range of adaptation to different levels of altitude, precipitation, and soils, and has important agronomic traits [2], particularly its high biomass production. Depending on the environment and cultivar characteristics, green biomass can reach 300 Mg ha − 1 year − 1 [3]; consequently, elephant grass is used for multiple purposes, including the production of bio-based compounds [4] and molecules with pharmaceutical and industrial applications [5–7]. It has been targeted by bioenergy programs, because its annual dry matter production is greater than that of sugarcane or eucalyptus, which are the most-used biomass energy sources in Brazil [2, 8].…”

Section: Introductionmentioning

confidence: 99%

Unraveling candidate genes underlying biomass digestibility in elephant grass (Cenchrus purpureus)

et al. 2019

View full text Add to dashboard Cite

BackgroundElephant grass [Cenchrus purpureus (Schumach.) Morrone] is used for bioenergy and animal feed. In order to identify candidate genes that could be exploited for marker-assisted selection in elephant grass, this study aimed to investigate changes in predictive accuracy using genomic relationship information and simple sequence repeats for eight traits (height, green biomass, dry biomass, acid and neutral detergent fiber, lignin content, biomass digestibility, and dry matter concentration) linked to bioenergetics and animal feeding.ResultsWe used single-step, genome-based best linear unbiased prediction and genome association methods to investigate changes in predictive accuracy and find candidate genes using genomic relationship information. Genetic variability (p < 0.05) was detected for most of the traits evaluated. In general, the overall means for the traits varied widely over the cuttings, which was corroborated by a significant genotype by cutting interaction. Knowing the genomic relationships increased the predictive accuracy of the biomass quality traits. We found that one marker (M28_161) was significantly associated with high values of biomass digestibility. The marker had moderate linkage disequilibrium with another marker (M35_202) that, in general, was detected in genotypes with low values of biomass digestibility. In silico analysis revealed that both markers have orthologous regions in other C4 grasses such as Setaria viridis, Panicum hallii, and Panicum virgatum, and these regions are located close to candidate genes involved in the biosynthesis of cell wall molecules (xyloglucan and lignin), which support their association with biomass digestibility.ConclusionsThe markers and candidate genes identified here are useful for breeding programs aimed at changing biomass digestibility in elephant grass. These markers can be used in marker-assisted selection to grow elephant grass cultivars for different uses, e.g., bioenergy production, bio-based products, co-products, bioactive compounds, and animal feed.

show abstract

Section: Introductionmentioning

confidence: 99%

Unraveling candidate genes underlying biomass digestibility in elephant grass (Cenchrus purpureus)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Results using various lignocellulosic hydrolysates for microbial lipid fermentation by various oleaginous species are summarized in Table 5. Compared to rice straw hydrolysates [39], sugarcane bagasse hydrolysates [40], wheat straw hydrolysates [14, 41], corn stover hydrolysates [17, 20, 42, 43], corncob residues hydrolysates [44], waste paper hydrolysates [45], laminaria residues hydrolysates [19], groundnut shell hydrolysates [46], cardoon stalks hydrolysates [47], and elephant grass hydrolysates [48], the present water hyacinth hydrolysates demonstrated inferior results of lipid production. The herbaceous biomass was nutrients-rich and resulted in very low lipid production.…”

Section: Resultsmentioning

confidence: 99%

Phosphate removal combined with acetate supplementation enhances lipid production from water hyacinth by Cutaneotrichosporon oleaginosum

Zhou

Tang

Zou

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Microbial lipids derived from various lignocellulosic feedstocks have emerged as a promising candidate for the biodiesel industry and a potential substitute for high value-added fats. However, lignocellulosic biomass, especially herbaceous biomass, such as water hyacinth, contains high concentrations of nitrogenous components. These compounds impede microbial lipid production, as lipid biosynthesis is commonly induced by imposing a nutrient deficiency, especially nitrogen starvation. Novel strategies and bioprocesses are pivotal for promoting lipid production from nitrogen-rich biomass. Results Here a combined strategy of phosphate removal and acetate supplementation was described for enhanced microbial lipid production on water hyacinth hydrolysates by Cutaneotrichosporon oleaginosum (formerly Cryptococcus curvatus ). Lipid production was significantly improved, when the phosphorus limitation and sugars/acetate co-utilization strategies were used separately. In this case, acetate and glucose were consumed simultaneously. Lipid production was observed by the combination of phosphate removal with acetate supplementation. Lipid titer, content, and yield were determined to be 7.3 g/L, 59.7% and 10.1 g/100 g raw water hyacinth, respectively. These data were increased by 4.2, 4.6, and 4.3 times, respectively, compared to those from the unprocessed hydrolysates. The fatty acid compositions of the resulting lipids bear a marked resemblance to those of rapeseed oil, indicating their applicability to the biodiesel industry. Conclusions The combination of phosphate removal and acetate supplementation was successful in significantly enhancing microbial lipid production. This strategy offers a valuable solution for nitrogen-rich lignocellulosic feedstocks utilization, which should foster more economical nitrogen-rich biomass-to-lipid bioprocesses.

show abstract

“…Therefore, to establish a sustainable microbial lipid production, extra endeavours are requisite such that yeast efficiently utilize renewable and low-cost carbon sources [12]. In recent years, inexpensive lignocellulosic carbon sources like rice straw hydrolysate [13], elephant grass hydrolysate [14], sugarcane bagasse hydrolysate [15], groundnut shell hydrolysate [16], wheat straw [17] and waste office paper hydrolysates [18] have been used for microbial lipid production. But, the conversion of these renewable feedstock like lignocellulosic materials into lipids in a cost-effective manner is a key challenge [19].…”

Section: Introductionmentioning

confidence: 99%

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Kumar

Deeba

Sauraj

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

show abstract

Use of elephant grass (Pennisetum purpureum) acid hydrolysate for microbial oil production byTrichosporon cutaneum

Cited by 11 publications

References 26 publications

Unraveling candidate genes underlying biomass digestibility in elephant grass (Cenchrus purpureus)

Unraveling candidate genes underlying biomass digestibility in elephant grass (Cenchrus purpureus)

Phosphate removal combined with acetate supplementation enhances lipid production from water hyacinth by Cutaneotrichosporon oleaginosum

Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

Contact Info

Product

Resources

About