2003
DOI: 10.1074/jbc.m302783200
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Abstract: Leucoanthocyanidin reductase (LAR) catalyzes the synthesis of catechin, an initiating monomer of condensed tannin or proanthocyanidin (PA) synthesis, from 3,4-cis-leucocyanidin and thus is the first committed step in PA biosynthesis. The enzyme was purified to near homogeneity from PA-rich leaves of the legume Desmodium uncinatum (Jacq.) DC, partially sequenced and the corresponding cDNA cloned. The identity of the enzyme was confirmed by expressing active recombinant LAR in Escherichia coli and in tobacco and… Show more

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Cited by 331 publications
(131 citation statements)
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“…ANR and LAR are both key enzymes of the branch of PAs biosynthesis pathway, which catalyze the formation of epicatechine or catechin respectively (Tanner et al, 2003;Xie et al, 2003). The results in our work showed that their expression levels decreased continuously during fruit developments (Figure 3 and 4).…”
Section: Expression Of Faanr and Falar In Strawberry Fruit Tissuessupporting
confidence: 60%
See 1 more Smart Citation
“…ANR and LAR are both key enzymes of the branch of PAs biosynthesis pathway, which catalyze the formation of epicatechine or catechin respectively (Tanner et al, 2003;Xie et al, 2003). The results in our work showed that their expression levels decreased continuously during fruit developments (Figure 3 and 4).…”
Section: Expression Of Faanr and Falar In Strawberry Fruit Tissuessupporting
confidence: 60%
“…Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are both key enzymes of the branch of PAs biosynthesis pathway (Tanner et al, 2003;Xie et al, 2003). They are responsible for the production of (-)-epicatechin and (+)-catechin, respectively (Xie et al, 2003;Pfeiffer et al, 2006).…”
Section: Introductionmentioning
confidence: 99%
“…1). The key enzymes for flavan-3-ol biosynthesis are anthocyanidin reductase (ANR), which catalyzes the reaction from anthocyanidin to 2,3-cisflavan-3-ols (e.g., (−)-epicatechin), and leucoanthocyanidin reductase (LAR), which catalyzes the reaction from leucoanthocyanidin to 2,3-trans-flavan-3-ols (e.g., (+)-catechin) (Tanner et al, 2003;Xie et al, 2003). The starter units of PAs are believed to be flavan-3-ols, while the origin of the extension units and the mechanism of PA polymerization have not been proven yet.…”
Section: Introductionmentioning
confidence: 99%
“…The enzymatic basis for the formation of the 2,3-trans, catechin-derived series of flavan-3-ols has been postulated to be related to the consecutive action of a dihydroflavonol reductase (DFR) (which produces a leucoanthocyanidin) and a leucoanthocyanidin reductase (LAR). Genetic determinants encoding LAR have been investigated [31]. More recent literature regarding Vitis vinifera L. cv Shiraz cells [28] reported that two enzymes, leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), can produce the flavan-3-ol monomers required for formation of proanthocyanidin polymers.…”
Section: Biosynthesis Of Catechins and Proanthocyanidinsmentioning
confidence: 99%