Photosystem I Is Indispensable for Photoautotrophic Growth, CO2 Fixation, and H2 Photoproduction inChlamydomonas reinhardtii

Redding, Kevin; Cournac, Laurent; Vassiliev, Ilya R.; Golbeck, John H.; Peltier, Gilles; Rochaix, Jean David

doi:10.1074/jbc.274.15.10466

Cited by 62 publications

(36 citation statements)

References 50 publications

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“…The absolute PS I content of whole cells can be determined by the light-induced absorbance increase at 832 nm due to the oxidation of P700 (27,31). On the basis of equal cell numbers, both the menA and menB mutant cells were found to contain 50 -60% of the photooxidizable P700 of the wild type cells (data not shown).…”

Section: Complementation Of Mena and Menb Mutant Strains Withmentioning

confidence: 99%

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Recruitment of a Foreign Quinone into the A1 Site of Photosystem I

Johnson¹,

Shen²,

Zybailov³

et al. 2000

Journal of Biological Chemistry

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127

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Genes encoding enzymes of the biosynthetic pathway leading to phylloquinone, the secondary electron acceptor of photosystem (PS) I, were identified in Synechocystis sp. PCC 6803 by comparison with genes encoding enzymes of the menaquinone biosynthetic pathway in Escherichia coli. Targeted inactivation of the menA and menB genes, which code for phytyl transferase and 1,4-dihydroxy-2-naphthoate synthase, respectively, prevented the synthesis of phylloquinone, thereby confirming the participation of these two gene products in the biosynthetic pathway. The menA and menB mutants grow photoautotrophically under low light conditions (20 E m ؊2 s ؊1 ), with doubling times twice that of the wild type, but they are unable to grow under high light conditions (120 E m ؊2 s ؊1 ). The menA and menB mutants grow photoheterotrophically on media supplemented with glucose under low light conditions, with doubling times similar to that of the wild type, but they are unable to grow under high light conditions unless atrazine is present to inhibit PS II activity. The level of active PS II per cell in the menA and menB mutant strains is identical to that of the wild type, but the level of active PS I is about 50 -60% that of the wild type as assayed by low temperature fluorescence, P700 photoactivity, and electron transfer rates. PS I complexes isolated from the menA and menB mutant strains contain the full complement of polypeptides, show photoreduction of F A and F B at 15 K, and support 82-84% of the wild type rate of electron transfer from cytochrome c 6 to flavodoxin. HPLC analyses show high levels of plastoquinone-9 in PS I complexes from the menA and menB mutants but not from the wild type. We propose that in the absence of phylloquinone, PS I recruits plastoquinone-9 into the A 1 site, where it functions as an efficient cofactor in electron transfer from A 0 to the iron-sulfur clusters.

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Section: Complementation Of Mena and Menb Mutant Strains Withmentioning

confidence: 99%

“…The measurement of P700 photooxidation in whole cyanobacterial cells was performed as described (27).…”

mentioning

confidence: 99%

Recruitment of a Foreign Quinone into the A1 Site of Photosystem I

Johnson¹,

Shen²,

Zybailov³

et al. 2000

Journal of Biological Chemistry

Self Cite

127

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“…The initial event in PSI assembly involves subunits encoded by the plastome: The formation of the heterodimer PsaA/PsaB is followed by association of PsaC, which is needed for further binding of PsaD and PsaE (Antonkine et al, 2003) and then a number of smaller subunits are added. While PSI in plants can accumulate in the absence of most small subunits, it is well known that mutants devoid of the PsaA/B dimer are unable to assemble a functional PSI core complex, although some peripheral subunits or detached Lhca can accumulate in the thylakoids (Redding et al, 1999;.…”

Section: Comparison Between Nox and Other Mutants Affected In The Biomentioning

confidence: 99%

The Arabidopsis nox Mutant Lacking Carotene Hydroxylase Activity Reveals a Critical Role for Xanthophylls in Photosystem I Biogenesis

et al. 2013

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Carotenes, and their oxygenated derivatives xanthophylls, are essential components of the photosynthetic apparatus. They contribute to the assembly of photosynthetic complexes and participate in light absorption and chloroplast photoprotection. Here, we studied the role of xanthophylls, as distinct from that of carotenes, by characterizing a no xanthophylls (nox) mutant of Arabidopsis thaliana, which was obtained by combining mutations targeting the four carotenoid hydroxylase genes. nox plants retained a-and b-carotenes but were devoid in xanthophylls. The phenotype included depletion of light-harvesting complex (LHC) subunits and impairment of nonphotochemical quenching, two effects consistent with the location of xanthophylls in photosystem II antenna, but also a decreased efficiency of photosynthetic electron transfer, photosensitivity, and lethality in soil. Biochemical analysis revealed that the nox mutant was specifically depleted in photosystem I function due to a severe deficiency in PsaA/B subunits. While the stationary level of psaA/B transcripts showed no major differences between genotypes, the stability of newly synthesized PsaA/B proteins was decreased and translation of psaA/B mRNA was impaired in nox with respect to wild-type plants. We conclude that xanthophylls, besides their role in photoprotection and LHC assembly, are also needed for photosystem I core translation and stability, thus making these compounds indispensable for autotrophic growth.

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“…The WT strain used in this work was isolated as an mt ϩ segregant of a cross between two strains isogenic to the 137c strain (Harris, 1989). PS I-deficient mutants were made by deletions of psaA in this strain as previously described (Fischer et al, 1996;Redding et al, 1999;Cournac et al, 2000b).…”

Section: Algal Materialsmentioning

confidence: 99%

In Vivo Interactions between Photosynthesis, Mitorespiration, and Chlororespiration in Chlamydomonas reinhardtii

et al. 2002

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Interactions between photosynthesis, mitochondrial respiration (mitorespiration), and chlororespiration have been investigated in the green alga Chlamydomonas reinhardtii using flash illumination and a bare platinum electrode. Depending on the physiological status of algae, flash illumination was found to induce either a fast (t 1/2 Ϸ 300 ms) or slow (t 1/2 Ϸ 3 s) transient inhibition of oxygen uptake. Based on the effects of the mitorespiratory inhibitors myxothiazol and salicyl hydroxamic acid (SHAM), and of propyl gallate, an inhibitor of the chlororespiratory oxidase, we conclude that the fast transient is due to the flash-induced inhibition of chlororespiration and that the slow transient is due to the flash-induced inhibition of mitorespiration. By measuring blue-green fluorescence changes, related to the redox status of the pyridine nucleotide pool, and chlorophyll fluorescence, related to the redox status of plastoquinones (PQs) in C. reinhardtii wild type and in a photosystem I-deficient mutant, we show that interactions between photosynthesis and chlororespiration are favored when PQ and pyridine nucleotide pools are reduced, whereas interactions between photosynthesis and mitorespiration are favored at more oxidized states. We conclude that the plastid oxidase, similar to the mitochondrial alternative oxidase, becomes significantly engaged when the PQ pool becomes highly reduced, and thereby prevents its over-reduction.Photosynthesis and respiration, the two major bioenergetic processes of living organisms, coexist within plant cells. Although the photosynthetic electron transport chain (ETC) is clearly restricted to chloroplasts, a respiratory ETC, originally thought to be solely located in mitochondria, has been suggested to be also present in chloroplasts (Bennoun, 1982;Peltier et al., 1987). This chloroplast-based respiration, which has been called chlororespiration to differentiate it from mitorespiration (Bennoun, 1982), probably has its origins in the cyanobacterial endosymbiotic ancestor of chloroplasts (Scherer, 1990). The concept of chlororespiration was initially proposed to account for the effects of respiratory inhibitors, and particularly of inhibitors of terminal oxidases, on photosynthesis in unicellular green algae (Bennoun, 1982). It was reported that cyanide, CO, or salicyl hydroxamic acid (SHAM) increased the redox level of the plastoquinone (PQ) pool, as measured by chlorophyll (Chl) fluorescence induction curves. In addition, flash illumination of algae was found to induce the inhibition of a respiratory process (Peltier et al., 1987). The insensitivity of the flash-induced O 2 signal to both antimycin A and SHAM, inhibitors of the mitochondrial ETC, and the requirement for PS I led to the conclusion that chlororespiration, rather than mitorespiration, was inhibited by flash excitation of PS I (Peltier et al., 1987). However, reduction of the PQ pool or inhibitions of O 2 uptake could also be explained by an inhibition of mitorespiration coupled to interactions between chloropla...

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Photosystem I Is Indispensable for Photoautotrophic Growth, CO2 Fixation, and H2 Photoproduction inChlamydomonas reinhardtii

Abstract: Certain Chlamydomonas reinhardtii mutants deficient in photosystem I due to defects in psaA mRNA maturation have been reported to be capable of CO 2 fixation, H 2 photoevolution, and photoautotrophic growth

Cited by 62 publications

References 50 publications

Recruitment of a Foreign Quinone into the A1 Site of Photosystem I

Recruitment of a Foreign Quinone into the A1 Site of Photosystem I

The Arabidopsis nox Mutant Lacking Carotene Hydroxylase Activity Reveals a Critical Role for Xanthophylls in Photosystem I Biogenesis

In Vivo Interactions between Photosynthesis, Mitorespiration, and Chlororespiration in Chlamydomonas reinhardtii

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