1984
DOI: 10.1073/pnas.81.4.1112
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Abstract: We have developed an in vitro transcription system that uses nuclei isolated from Lemna gibba G-3. The in vitro transcripts include sequences homologous to hybridization probes for the small subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39], the light-harvesting chlorophyll a/b-protein, and rRNA. Light-harvesting chlorophyll a/b-protein se-quences are transcribed to a greater extent in nuclei isolated from plants grown in darkness with 2 min of red… Show more

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Cited by 255 publications
(122 citation statements)
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“…The transcription of cab (1 1,13,20) and rbcS (5,6,20) genes are regulated by phytochrome in angiosperms. To examine the involvement of phytochrome in the expression of the two genes of interest, in terms of levels of the respective transcripts, 14-d-old dark-grown seedlings were exposed to red light and far-red light.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The transcription of cab (1 1,13,20) and rbcS (5,6,20) genes are regulated by phytochrome in angiosperms. To examine the involvement of phytochrome in the expression of the two genes of interest, in terms of levels of the respective transcripts, 14-d-old dark-grown seedlings were exposed to red light and far-red light.…”
Section: Resultsmentioning
confidence: 99%
“…This phenomenon suggests that the development of chloroplasts can proceed without illumination in conifers. The expression of cab and rbcS is positively regulated by a phytochromemediated response (5,6,11,13,20), but the fact that the cotyledons of dark-grown seedlings of coniferous plants contain chlorophylls suggests that some genes, such as cab and rbcS, are expressed even in darkness. In fact, Alosi et al (1) recently showed the presence of LHCP and cab transcript in dark-grown Douglas-fir seedlings.…”
mentioning
confidence: 99%
“…It has been known for some time that rbcS genes are regulated by light and that this process is at least in part mediated at the level of transcription, e.g. in pea (Gallagher et al, 1985), Lemna (Silverthorne and Tobin, 1984), soybean (Berry-Lowe and Meagher, 1985), and rye (Emst et al, 1987). Light can also affect gene expression at a posttranscriptional level (Jenkins, 1991), and some data suggest that photoregulation of rbcS gene expression can also be exercised at the level of transcript stability (Emst et al, 1987;Wanner and Gruissem, 1991).…”
Section: 900987mentioning
confidence: 99%
“…Among the bestcharacterized phytochrome-regulated genes are the CAB, which encode a structural component of the lightharvesting complex. C A B expression is regulated at the transcriptional level by phytochrome (Silverthorne and Tobin, 1984). The induction level of C A B versus photon fluente of red light has a biphasic fluence-response curve in pea (Pisum sativum L.; Kaufman et al, 1984), indicating that the level of C A B transcript is affected by light in the VLF and LF range.…”
mentioning
confidence: 99%