1980
DOI: 10.1073/pnas.77.6.3346
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Abstract: The locations of DNA binding by the proteins involved with positive and negative regulation of transcription initiation of the L-arabinose operon in Escherichia coli have been determined by the DNase I protection method. Two cyclic AMP receptor protein sites were found, at positions -78 to -107 and -121 to -146, an araCprotein-arabinose binding site was found at position -40 to -78, and an araC protein-fucose binding site was found at position -106 to -144. These locations, combined with in vivo data on induct… Show more

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Cited by 168 publications
(132 citation statements)
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References 28 publications
(25 reference statements)
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“…The lac operon (which apparently has only one mRNA start site) requires but one CAP molecule, bound at -50 to -70, for transcriptional stimulation (4,16). CAP binds between positions -80 and -100 at the araBAD operon; however, the araC protein, which binds between CAP and RNA polymerase, is also necessary for expression of the operon (26). There are strildng sequence homologiesbetween the CAPbinding sites of gal (-30'to -44 and -48 to -75) (2,22), lac (-50 to -75) (7), and araBAD (-82 to -92) (27,28).…”
mentioning
confidence: 99%
“…The lac operon (which apparently has only one mRNA start site) requires but one CAP molecule, bound at -50 to -70, for transcriptional stimulation (4,16). CAP binds between positions -80 and -100 at the araBAD operon; however, the araC protein, which binds between CAP and RNA polymerase, is also necessary for expression of the operon (26). There are strildng sequence homologiesbetween the CAPbinding sites of gal (-30'to -44 and -48 to -75) (2,22), lac (-50 to -75) (7), and araBAD (-82 to -92) (27,28).…”
mentioning
confidence: 99%
“…Both the level of protein required for binding and the pattern of protection from nuclease digestion remain unchanged, however it is not yet known whether these experiments, under the conditions employed, could detect some physiologically important alterations in binding. While all the mutations lie just within the DNA region protected by araC protein (7,8), the insertion mutations duplicate DNA sequence so that the binding sites remain largely intact (Fig. 2) (20,21).…”
Section: Discussionmentioning
confidence: 99%
“…DNase I protection and galactokinase assay DNase I protection was as described (7). Approximately 20 ng of ara DNA fragment was incubated for 10 minutes at 370 with araC protein in 100 4l 20 mM Tris-HCl pH 7.5, 50 mM KC1, 2.5 mM MgC12, 0.1 mM DTT, 100 mM L-arabinose and 25 pg/ml BSA.…”
Section: Base Insertions and Deletionsmentioning
confidence: 99%
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“…The cAMP. CRP complex interacts with specific DNA sites at or near promotors [6,7], stimulating the initiation of mRNA synthesis [8,9]. The mechanism of the activation of transcription, however, is still poorly understood.…”
Section: Introductionmentioning
confidence: 99%