High CD4+ T-Cell/B-Cell Ratio in the Paranasal Sinus Mucosa of Patients with Eosinophilic Chronic Rhinosinusitis

Abstract

Introduction Chronic rhinosinusitis (CRS) is commonly classified based on the presence or absence of nasal polyps (NPs). Eosinophil infiltration is observed in NPs of patients in Western countries. In contrast, in East Asian countries, including Japan, CRS with NPs (CRSwNP) is subdivided based on the presence (eosinophilic CRS [ECRS]) or absence (non-eosinophilic CRS [NECRS]) of eosinophils in NPs. However, detailed analyses of other immune cells, such as lymphocytes, in NPs have not been performed. Therefore, clarification of the types of cells that infiltrate NPs is important to understand CRS pathogenesis. Objectives We analyzed the lymphocytes that infiltrate the paranasal sinus mucosa of ECRS and NECRS patients. Methods Eighteen patients with CRSwNP participated in this study, out of whom 6 were NECRS patients, and 12 were ECRS patients. The mucosa specimens, collected from patients during sinus surgeries, were subjected to collagenase treatment to prepare single cell suspensions. Then, mononuclear cells were isolated, and CD4+ T, CD8+ T, and CD20+ B-cell populations were examined using flow cytometry. Results In both NECRS and ECRS patients, CD8+ T-cells were dominant over CD4+ T-cells. Notably, CD4+ T-cell/B-cell ratio, but not CD8+ T-cell/B-cell or CD4+ T-cell/CD8+ T-cell ratios, was significantly higher in ECRS patients than in NECRS patients. Conclusion The CD4+ T-cell/B-cell ratio can be used as a potential indicator to differentiate between ECRS and NECRS.

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“…CD8+ T cell은 바이러스 침입에 대 한 면역력과 관련되어 있다. 일반적으로 CD4+ T cell과 CD8+ T cell의 분포는 역의 상관관계를 가지며 변화된다고 알려져 특정 스트레스 반응이나 질환을 진단하기 위해 CD4+ T cell:CD8+ T cell ratio를 바이오 마커로써 사용하기 도 한다 (Inoue et al, 2021). 유의미한 차이가 없었던 전체 T 세포 분포에 비해 사료 내 미세조류를 처리한 그룹에서 CD4+ T cell 양의 증가는 상대적으로 CD8+ T cell의 분포 감소를 유도한 것으로 보인다.…”